Clinical blood analysis of the great tit Parus major

Drahulian, M. V.; Чаплыгина, А. Б.; Savynska, Nadiia; Kostenko, S.; Buchek, P. V.

doi:10.15587/2313-8416.2018.127387

Cited by 3 publications

(2 citation statements)

References 1 publication

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“…The species that exhibited the highest damage in terms of micronucleus and nuclear abnormalities are not the most suitable examples for inferring results regarding forest degradation, as these species were not distributed throughout the entire study area. Our data show the same variability reported for other species in different studies, given by the characteristics of the species and their growth conditions (Bolognesi & Hayashi, 2011 ; M. Drahulian et al., 2018 ; Silveira et al., 2022 ; Souto et al., 2018 ). One of the limitations of the study is the fact that we cannot establish that the effects of system degradation affect finding different types of birds, which meant that we could only monitor only four species in the three localities.…”

Section: Discussionsupporting

confidence: 89%

Chronic Degradation of Seasonally Dry Tropical Forests Increases the Incidence of Genotoxicity in Birds

Cevallos‐Solorzano,

Bailon‐Moscoso,

Ordóñez‐Delgado

et al. 2023

GeoHealth

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Multiple studies have shown that exposure to pollutants can increase genotoxic damage in different taxa. However, to our knowledge, the effects of environmental stress have been explored little. In certain stressful ecosystems, such as seasonally dry tropical forests, the combined effects of anthropogenic activities and ongoing global changes can cause an increase in environmental stresses, in turn, may trigger physiological and genetic effects on biodiversity. The present aims to assess changes in the prevalence of genotoxic damage in birds within three states of forest degradation in the Tumbesian Region of Western Ecuador. We used blood samples from 50 bird species to determine the frequency of micronucleus and nuclear abnormalities in erythrocytes. Our results revealed a significant impact of forest degradation on the occurrence probability of micronucleus and nuclear abnormalities at the community level. Localities with higher levels of degradation exhibited higher levels of abnormalities. However, when analyzing the dominant species, we found contrasting responses. While Lepidocolaptes souleyetii showed a reduction in the proportion of nuclear abnormalities from the natural to shrub‐dominated localities Troglodytes aedon and Polioptila plumbea showed an increase for semi‐natural and shrub‐dominated respectively. We concluded that the degradation process of these tropical forests increases the stress of bird community generating genotoxic damage. Bird responses seem to be species‐specific, which could explain the differences in changes in bird composition reported in other studies.

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Section: Discussionsupporting

confidence: 89%

Chronic Degradation of Seasonally Dry Tropical Forests Increases the Incidence of Genotoxicity in Birds

Cevallos‐Solorzano,

Bailon‐Moscoso,

Ordóñez‐Delgado

et al. 2023

GeoHealth

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“…To obtain a smear, we introduced a capillary with a light screw-like motion under the eyeball, closer to the corner of the eye. The blood coming through the capillary was placed on a properly marked watchglass (Drahulian et al, 2018b).…”

Section: Haematological Methods Of Researchmentioning

confidence: 99%

The physiological and genetic differences between flycatchers (Ficedula albicollis vs. Ficedula hypoleuca)

et al. 2018

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The paper studies interspecies physiological and genetic differences between the white-collared flycatcher and the pied flycatcher of the genus Ficedula. The fact that the flycatchers are capable of interspecies hybridization is one more reason for particular interest to these species. Using our own-developed method of taking venous blood from the eye’s sinus, we took blood samples from birds in the protected area of Homilshanski Forests, Kharkiv, Ukraine, to study their leukogram patterns. The bird feathers were also collected for genetic analysis -- to identify interspecies differences with application of the ISSR technique. It was revealed that the percentage of heterophiles in the nestlings of the pied flycatcher was lower than in the nestlings of the white-collared flycatcher. The micronucleus test did not reveal any significant difference in the interspecies groups. The spectra of amplification products obtained with the primer (AGC)6 G showed that the white-collared flycatchers had a more heterogeneous structure. The study of the leukogram, micronucleus test, and the ISSR analysis can be especially effective in the study of intra-species genetic differentiation.

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Sex identification of different species of wild birds using a single universal protocol to the bird sexing method based on gene polymorphism

Rudaуа

Katerynych

Drahulian³

et al. 2020

Regul. Mech. Biosyst.

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This article presents an elaboration of the protocol for the method of sexing wild birds based on the polymorphism of the CHD gene using P2/P8 primer for Common Pheasant – Phasianus colchicus (Linnaeus, 1758; Galliformes, Phasianidae); Silver Lofur or Silver Pheasant – Lophura nycthemera (Linnaeus, 1758; Galliformes, Phasianidae), Budgerigar – Melopsittacus undulatus (Shaw, 1805; Psittaciformes, Psittacidae), Herring Gull – Larus argentatus (Pontoppidan, 1763; Charadriiformes, Laridae), and White Stork – Ciconia ciconia (Linnaeus, 1758; Ciconiiformes, Ciconiidae). Blood samples were taken from Common Pheasant, Silver Pheasant and White Stork using the “drop of blood on paper” method. For the Budgerigar and the Herring Gull, DNA was isolated from the feather follicle. To isolate DNA, a commercial NeoPrep 100 DNA reagent kit (Neogen, Ukraine) was used. Primers P2/P8 were used for PCR; PCR was performed using GenPac PCR Core reagents (Neogen, Ukraine). We selected the optimal amount of Tag polymerase, the amount of DNA and primers and, according to the amount of reagents, set acceptable amplification modes and electrophoresis agarose gel percentage. Prior to PCR, additional DNA gel electrophoresis purification is proposed, which increases the percentage of positive sex determination results. It was found that the ideal mixture for the 5 bird species was an amplification mixture (total volume 20 µL, containing 1 U Tag polymerase, 100 ng DNA and 0.6 µM of each primer). The amplified CHD-Z fragment of Common and Silver pheasants is of ~340 n. p., CHD-W ~360 n. p. Herring Gull and Budgerigar have ~350 n. p. of CHD-Z length, and ~400 n. p. of CHD-W length, White Stork has its CHD-Z of ~ 370 n. p. long. It is advisable to investigate the genome of the experimental bird species using horizontal electrophoresis in agar’s gel with the concentration of 5%, which makes it possible to clearly visualize the female genotype. The universal protocol of the method of sex determination based on polymorphism of the CHD gene for the 5 studied bird species is described. These results of the study led to the conclusion that for the simultaneous sexing of several species of birds, it is advisable to develop a unified protocol for determining the status of the CHD gene, with the aim of clarifying the gender, as well as new approaches in ornithology and ecology aimed at determining interspecific differences associated with gene polymorphism. Identification of differences in fragment sizes may be useful for identifying the species in cases when birds form mixed pairings for taxonomic and phylogenetic comparisons.

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Clinical blood analysis of the great tit Parus major

Cited by 3 publications

References 1 publication

Chronic Degradation of Seasonally Dry Tropical Forests Increases the Incidence of Genotoxicity in Birds

Chronic Degradation of Seasonally Dry Tropical Forests Increases the Incidence of Genotoxicity in Birds

The physiological and genetic differences between flycatchers (Ficedula albicollis vs. Ficedula hypoleuca)

Sex identification of different species of wild birds using a single universal protocol to the bird sexing method based on gene polymorphism

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