Clinical Cases and Serum Biochemical Profiles of Horses Naturally Infected with Dourine in Western Arsi Zone, Ethiopia

Gizaw, Yonas; Ashenafi, Hagos; Demssie, Tilaye

doi:10.4172/2157-7579.1000528

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Ferreira et al (2023) and Desquesnes et al (2013), there is an urgent requirement for diagnostic tools and mass screenings in the field due to the high prevalence of these diseases. Gizaw et al (2021) have pointed out that recent outbreaks of trypanosome infections highlight the genuine risk of equine trypanosomosis spreading globally. Therefore, it is crucial to develop accurate and specific diagnostic methods to ensure the safety of international horse trade.…”

Section: Discussionmentioning

confidence: 99%

Development of a Test System Based on Recombinant GM6 Antigen from Trypanosoma evansi for the Determination of Surra in Horses

Akhmetsadykov,

Kydyrov,

Akhmetzhanova

et al. 2023

JAHP

View full text Add to dashboard Cite

| Trypanosomosis, such as Surra, causes significant damage to livestock worldwide. The development of new serological diagnostic methods is highly relevant. The primary objective of this study was to produce a recombinant GM6 antigen derived from T. evansi and investigate its immunological properties for the detection and diagnosis of trypanosomosis. To create a recombinant antigen, bioinformatic analysis of the primary structure of T. evansi GM6 antigen from several regions was carried out. The protein sequence was reverse translated to the nucleotide sequence, after which the gene was synthesized using solid-phase method. The gene fragment, encoding the GM6 protein, was inserted or cloned into the pET28 expression vector after synthesis. The obtained sequences were checked by sequencing for correspondence to the matrix molecule. For transformation E. coli BL21 (DE3) strain was used. To assess the efficacy of the antigen, immunoassay analysis and blot-hybridization techniques were employed. Obtained results showed that 1mM isopropyl ß-D-1-thiogalactopyranoside induction at 2℃ for 18 hours was the most optimal condition for expression of recombinant GM6 T. evansi antigen. From a 500 ml of growth medium, a total of 3.4 mg of recombinant protein was successfully obtained. A recombinant GM6 antigen of T. evansi encoding a 30 kDa polypeptide was developed, which has diagnostically significant sensitivity in the enzyme immunoassay at a dilution of 1:6400 with sera of horses and donkeys infected with trypanosomosis. The proposed test system can be used for diagnosis of trypanosomosis in the early stages of the disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of a Test System Based on Recombinant GM6 Antigen from Trypanosoma evansi for the Determination of Surra in Horses

Akhmetsadykov,

Kydyrov,

Akhmetzhanova

et al. 2023

JAHP

View full text Add to dashboard Cite

show abstract

An Update on the African Trypanosomosis Status in South Africa

Innocentia Molefe-Nyembe

2024

Infectious Diseases

View full text Add to dashboard Cite

The cold climate of South Africa likely contributed to the historical eradication of the vector for Trypanosoma brucei rhodesiense in the 1950s, leading to the absence of the disease. However, studies have recorded the presence of vectors for Trypanosoma congolense and Trypanosoma brucei brucei, though they remain confined to the warmer provinces. Given the undeniable effects of global warming, with South Africa warming rapidly, this chapter updates the status of Trypanosoma spp. and their pathogenicity and describes the potential impact of this warming trend on the disease in South Africa.

show abstract

Clinical Cases and Serum Biochemical Profiles of Horses Naturally Infected with Dourine in Western Arsi Zone, Ethiopia

Cited by 2 publications

References 0 publications

Development of a Test System Based on Recombinant GM6 Antigen from Trypanosoma evansi for the Determination of Surra in Horses

Development of a Test System Based on Recombinant GM6 Antigen from Trypanosoma evansi for the Determination of Surra in Horses

An Update on the African Trypanosomosis Status in South Africa

Contact Info

Product

Resources

About