Clinical characteristics and antibodies against Echinococcus granulosus recombinant antigen P29 in patients with cystic echinococcosis in China

Jia, Tao; Du, Xiancai; Liu, Kejun; Wang, Chan; Lv, Yongxue; Wang, Minglei; Yang, Zhiqi; Yang, Jihui; Li, Shasha; Wu, Changyou; Li, Minghao; Zhao, Wei

doi:10.1186/s12879-022-07597-8

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…CE is considered one of the 17 neglected tropical diseases reported by the World Health Organization, and this helminth zoonotic disease is an important public health problem occurring worldwide (14). Kurdistan province is one of the important areas of animal husbandry in Iran.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence and Risk Factors Associated With Human Cystic Echinococcosis in Baneh, West of Iran

Moghimi,

Bakhtiari,

Matini

et al. 2023

Avicenna J Clin Microbiol Infect

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Background: Cystic echinococcosis (CE) is a zoonotic helminth infection that has great health and economic importance worldwide. Iran is considered one of the endemic areas of this parasitic infection. The aim of this study was to determine the seroprevalence and risk factors of human CE in Baneh, west of Iran. Methods: From March to May 2022, 460 individuals who attended health centers in Baneh were tested for the anti-Echinococcus immunoglobulin G (IgG) antibody by enzyme-linked immunosorbent assay. The data were analyzed by Chi-square and binary logistic regression tests. Results: Fourteen (3.04%) participants (95% CI: 1.49–4.59%) had the anti-Echinococcus IgG antibody. The average (±standard deviation) age of participants was 40.9 (16.8) years, and most of them belonged to the age group of 35–49 years (33.9%) with the highest rate of infection (4.5%). In terms of other demographic variables, seropositivity to CE was higher in women (3.8%) and people who lived in rural areas (5.1%), were housewives (3.6%), and were illiterate (4%) (P>0.05). Binary logistic regression analysis revealed that only keeping dogs at home was a risk factor for CE (adjusted odds ratio: 31.407; 95% CI: 5.343–184.590%; P<0.001). Conclusion: Direct contact with dogs is the main method of CE transmission. Therefore, public health education and the deworming of dogs can be effective in preventing and controlling CE.

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Section: Discussionmentioning

confidence: 99%

Seroprevalence and Risk Factors Associated With Human Cystic Echinococcosis in Baneh, West of Iran

Moghimi,

Bakhtiari,

Matini

et al. 2023

Avicenna J Clin Microbiol Infect

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Magnetic resonance imaging and next-generation sequencing for the diagnosis of cystic echinococcosis in the intradural spine: a case report

Lao,

Hu,

et al. 2023

J Med Case Reports

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Background Cystic echinococcosis (CE) is a parasitic zoonotic disease caused by the larval stage of Echinococcus granulosus. The liver and lungs are the most common sites for infection. Infection of the intradural spine is rare. Case presentation A 45-year-old woman of Han ethnicity presented with a chronic history of recurrent lumbar pain. Magnetic resonance imaging of the lumbar spine revealed the classical characteristic of multiple cystic lesions of variable sizes, manifesting a “bunch of grapes” appearance, localized within the spinal canal at the L4–L5 vertebral level. In the meanwhile, metagenomic next-generation sequencing identified Echinococcosis granulosa. The patient underwent surgery to remove the cyst entirely and subsequently took albendazole 400 mg orally twice daily for 6 months. Conclusion Spinal CE should be suspected in patients with multiple spinal cystic lesions and zoonotic exposure. metagenomic next-generation sequencing serves as a robust diagnostic tool for atypical pathogens, particularly when conventional tests are inconclusive. Prompt and aggressive treatment for spinal cystic echinococcosis is imperative, and further research is warranted for improved diagnostic and therapeutic strategies.

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Enhancing the therapeutic potential of P29 protein-targeted monoclonal antibodies in the management of alveolar echinococcosis through CDC-mediated mechanisms

Zhang,

Li,

Hou

et al. 2024

PLoS Pathog

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Alveolar echinococcosis (AE) is a highly lethal helminth infection. Current chemotherapeutic strategies for AE primarily involve the use of benzimidazoles (BZs) such as mebendazole (MDZ) and albendazole (ABZ), which exhibit limited efficacy. In a previous study, the vaccine of recombinant Echinococcus granulosus P29 (rEgP29) showed significant immunoprotection against E. granulosus in both mice and sheep. In the current study, we utilized hybridoma technology to generate five monoclonal antibodies (mAbs) against P29, among which 4G10F4 mAb exhibited the highest antigen-specific binding capacity. This mAb was selected for further investigation of anti-AE therapy, both in vivo and in vitro. In vitro, 4G10F4 inhibited a noteworthy inhibition of E. multilocularis protoscoleces and primary cells viability through complement-dependent cytotoxicity (CDC) mechanism. In vivo, two experiments were conducted. In the first experiment, mice were intraperitoneally injected with Em protoscoleces, and subsequently treated with 4G10F4 mAb (2.5/5/10 mg/kg) at 12 weeks postinfection once per week for 8 times via tail vein injection. Mice that were treated with 4G10F4 mAb only in dosage of 5mg/kg exhibited a significant lower mean parasite burden (0.89±0.97 g) compared to isotype mAb treated control mice (2.21±1.30 g). In the second experiment, mice were infected through hepatic portal vein and treated with 4G10F4 mAb (5mg/kg) at one week after surgery once per week for 8 times. The numbers of hepatic metacestode lesions of the 4G10F4 treatment group were significantly lower in comparison to the isotype control group. Pathological analysis revealed severe disruption of the inner structure of the metacestode in both experiments, particularly affecting the germinal and laminated layers, resulting in the transformation into infertile vesicles after treatment with 4G10F4. In addition, the safety of 4G10F4 for AE treatment was confirmed through assessment of mouse weight and evaluation of liver and kidney function. This study presents antigen-specific monoclonal antibody immunotherapy as a promising therapeutic approach against E. multilocularis induced AE.

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Clinical characteristics and antibodies against Echinococcus granulosus recombinant antigen P29 in patients with cystic echinococcosis in China

Cited by 3 publications

References 45 publications

Seroprevalence and Risk Factors Associated With Human Cystic Echinococcosis in Baneh, West of Iran

Seroprevalence and Risk Factors Associated With Human Cystic Echinococcosis in Baneh, West of Iran

Magnetic resonance imaging and next-generation sequencing for the diagnosis of cystic echinococcosis in the intradural spine: a case report

Enhancing the therapeutic potential of P29 protein-targeted monoclonal antibodies in the management of alveolar echinococcosis through CDC-mediated mechanisms

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