Clinical Characteristics of Stenotrophomonas maltophilia Keratitis

Wu, An-Lun; Yeh, Lung‐Kun; David, H. K.; Chen, Phil Y. F.; Lin, Hsin-Chiung; Sun, Chi-Chin; Tan, Hsin-Yuan; Chen, Hung-Chi; Chen, Shin‐Yi; Hsiao, Ching-Hsi

doi:10.1097/ico.0000000000000855

Cited by 24 publications

(31 citation statements)

References 28 publications

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“…Clinical evidence has shown that S. maltophilia can cause nosocomial infections in immunocompromised hosts, such as respiratory system infections, joint infections, and skin infections [3]. Wu et al [4] also found that the dominant flora in keratitis infection was S. maltophilia. In several of the China Antimicrobial Surveillance Network (CHINET) reports, among the nonfermentative gram-negative bacilli, S. maltophilia was the third largest per year, just after Pseudomonas aeruginosa and Acinetobacter baumannii, and the number of clinical isolates showed an upward trend [5].…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

et al. 2020

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Background Stenotrophomonas maltophilia (S. maltophilia) is an important opportunistic pathogen that can be isolated in hospitals. With the abuse of broad spectrum antibiotics and invasive surgical devices, the rate of S. maltophilia infection is increasing every year. This study was an epidemiological analysis of the clinical and molecular characteristics of S. maltophilia infection in a Chinese teaching hospital. The goal was to obtain a comprehensive understanding of the status of S. maltophilia infection to provide strong epidemiological data for the prevention and treatment of S. maltophilia infection. Results A total of 93 isolates from Renji Hospital affiliated with the Shanghai Jiaotong University School of Medicine were included, in which 62 isolates were from male patients. In addition, 81 isolates were isolated from sputum samples. A total of 86 patients had underlying diseases. All patients received antibiotics. Multilocus sequence typing (MLST) analysis indicated that 61 different sequence types (STs) were found (including 45 novel STs), and MLST did not show significantly dominant STs. Pulsed field gel electrophoresis (PFGE) results showed that 93 isolates could be divided into 73 clusters, and they also showed weak genetic linkages between isolates. The resistant rates to trimethoprim/sulfamethoxazole (TMP/SMX) and levofloxacin were 9.7 and 4.3%, respectively, and all isolates were susceptible to minocycline. Four virulence gene’s loci Stmpr1, Stmpr2, Smf-1, and Smlt3773 were positive in 79.6, 91.4, 94.6, and 52.7% of the isolates, respectively. Three biofilm genes rmlA, spgM, and rpfF were positive in 82.8, 92.5, and 64.5% of the isolates, respectively. Mean biofilm forming level of OD492 was 0.54 ± 0.49. We did not find any significant difference between different genders and different age-groups. We retrospectively analyzed data from patients in the intensive care unit (ICU) and the control group. The independent risk factors of those who were infected in the ICU included immunosuppression and the increased antibiotic usage. Conclusions Most of the patients had prior medical usage histories and baseline diseases. The positive rate of virulence genes was high, the drug resistance rate of S. maltophilia was low, and the biofilm formation ability was strong. The increased use of antibiotics was an independent risk factor for S. maltophilia infection, which should receive more attention. No obvious clonal transmissions were found in the same departments.

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Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

et al. 2020

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“…Clinical evidence has shown that S. maltophilia can cause nosocomial infections in immunocompromised hosts such as respiratory system infection, joint infection and skin infection. Wu et al(Wu et al, 2016) also found that the dominant flora of S. maltophilia in the infection of keratitis. In many China Antimicrobial Surveillance Network (CHINET) reports, S. maltophilia was the third largest fungus among non-fermentative Gram-negative bacilli, just after Pseudomonas aeruginosa and Acinetobacter baumannii, and the number of clinical isolates showed an upward trend (Hu et al, 2018).…”

Section: Introductionmentioning

confidence: 83%

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Duan

Qin

Liu

et al. 2020

Preprint

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Background: Stenotrophomonas maltophilia (S. maltophilia) is an important opportunistic pathogen obtained in hospitals. With the abuse of broad spectrum antibiotics and invasive surgical devices, the rate of S. maltophilia infections is increasing year by year. This study is aimed at epidemiological analysis of the clinical and molecular characteristics of S. maltophilia infections in a Chinese teaching hospital. We wish to have a comprehensive understanding of the status of S. maltophilia infections, in order to provide strong epidemiological data for the prevention and treatment of S. maltophilia infections. Results: 93 isolates from the Renji Hospital affiliated to Shanghai Jiaotong University School of Medicine were included, in which 62 strains were from male patients and 81 isolates were cultured from sputum samples. 86 patients had underlying diseases. All patients have received antibiotic therapeutics. MLST analysis indicated that 61 different sequence types (STs) were found (including 45 novel STs), and MLST did not show significantly adominant STs. PFGE showed weak genetic linkage between strains. The resistant rates of Trimethoprim/sulfamethoxazole (TMP/SMX) and levofloxacin were 9.7% and 4.3%. All the strains were susceptible to minocycline. The positive rates of the four virulence genes Stmpr1, Stmpr2, Smf-1 and Smlt3773 locus were 79.6%, 91.4%, 94.6% and 52.7%. The positive rates of the three biofilm genes rmlA, spgM and rpfF were 82.8%, 92.5% and 64.5%. The mean biofilm forming level of OD492 was 0.54 ± 0.49, and there was no significant difference between genders and among different age groups. The data from patients with ICU and the control group were analyzed retrospectively, and the risk factors infected in ICU included the hypoimmunity and the increase of the use of antibiotics were independent risk factors. Conclusion: Most of the patients had prior medical usage history and baseline diseases. The carrying rate of virulence genes was high, the drug resistance rate of S. maltophilia was low, and the biofilm formation ability was strong. The increased use of antibiotics is an independent risk factor for S. maltophilia infection, which should be payed more attention. No obvious clonal transmission was found in the same department.

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“…Clinical evidence has shown that S. maltophilia can cause nosocomial infections in immunocompromised hosts, such as respiratory system infections, joint infections, and skin infections [3]. Wu et al [4] also found that the dominant ora in keratitis infection was S. maltophilia. In many China Antimicrobial Surveillance Network (CHINET) reports , among nonfermentative gram-negative bacilli, S. maltophilia was the third largest one every year, just after Pseudomonas aeruginosa and Acinetobacter baumannii, and the number of clinical isolates showed an upward trend [5].…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Duan

Qin

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Stenotrophomonas maltophilia (S. maltophilia) is an important opportunistic pathogen that can be isolated in hospitals. With the abuse of broad spectrum antibiotics and invasive surgical devices, the rate of S. maltophilia infection is increasing every year. This study is an epidemiological analysis of the clinical and molecular characteristics of S. maltophilia infection in a Chinese teaching hospital. The goal is to obtain a comprehensive understanding of the status of S. maltophilia infection in order to provide strong epidemiological data for the prevention and treatment of S. maltophilia infection.Results: A total of 93 isolates from Renji Hospital affiliated to Shanghai Jiaotong University School of Medicine were included, in which 62 isolates were from male patients and 81 isolates were isolated from sputum samples. A total of 86 patients had underlying diseases. All of the patients had received antibiotic therapeutics. The multilocus sequence typing (MLST) analysis indicated that 61 different sequence types (STs) were found (including 45 novel STs), and the MLST did not show significantly dominant STs. Pulsed field gel electrophoresis (PFGE) results showed the 93 isolates could be divided into 73 clusters, and they also showed weak genetic linkage between isolates. The resistant rates to Trimethoprim/sulfamethoxazole (TMP/SMX) and levofloxacin were 9.7% and 4.3%, respectively, and all of the isolates were susceptible to minocycline. The positive rates of the four virulence genes Stmpr1, Stmpr2, Smf-1, and Smlt3773 locus were 79.6%, 91.4%, 94.6%, and 52.7%, respectively. The positive rates of the three biofilm genes rmlA, spgM, and rpfF were 82.8%, 92.5%, and 64.5%, respectively. The mean biofilm forming level of OD492 was 0.54 ± 0.49, and there was no significant difference between different genders and different age groups. Data from patients in the intensive care unit (ICU) and the control group were analyzed retrospectively, and the independent risk factors of those who were infected in the ICU included immunosuppression and the increased of the antibiotics usage.Conclusions: Most of the patients had prior medical usage histories and baseline diseases. The positive rate of virulence genes was high, the drug resistance rate of S. maltophilia was low, and the biofilm formation ability was strong. The increased use of antibiotics was an independent risk factor for S. maltophilia infection, which should receive more attention. No obvious clonal transmission was found in the same department.

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Clinical Characteristics of Stenotrophomonas maltophilia Keratitis

Abstract: S. maltophilia keratitis primarily affects patients with compromised ocular surfaces. S. maltophilia demonstrates in vitro susceptibility and a strong clinical response to fluoroquinolones.

Cited by 24 publications

References 28 publications

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital

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