Clinical chemistry, haematology, immune response and histological evaluation of rabbits after immunisation and challenge with rabbit haemorrhagic disease (RHD) virus

Abstract

Following their immunisation and infection with a VSHI-CN-6 viral strain, a group of 15 rabbits were examined in a study of Rabbit Haemorrhagic Disease (RHD). Serum samples were collected from the external ear vein at 0, 15, 30 and 60 days post-immunisation. The recorded platelet numbers were closer to the lower physiological limit, indicating a mild thrombocytopenia, with values ranging from 26.6 to 30.43×10 4 /mm 3 . The phagocytic index revealed significant differences (P<0.001) between the mean values obtained before vaccination (day 0) and the 3 post-vaccination measurements, confirming the increase in phagocytic capacity after immunisation. Additionally, the serum lysozyme average value equalled 9.14 mg/mL post-vaccination. The analysis of variance revealed significant statistical differences (P<0.05) between the average values obtained before vaccination (0) and the post-vaccination values, measured on day 14 and 30, respectively. The morphology of the samples collected from the main organs involved in immune protection, spleen and gastric and portal lymph nodes highlighted changes corresponding to the post-vaccination immunological response. The white pulp of the spleen appeared as a diffuse lymphoid tissue, presenting with primary and secondary lymphoid follicles. The ratio of white/red pulp was in favour of the white pulp and multiple lymphoid follicles were present, indicating their reactivation. In the medullary area of gastric and portal lymph nodes, narrow lymphoid cords, circumscribed by relatively large lymphatic sinuses, and well defined lymphocytolysis were observed. Moreover, the exudate and lymphoid follicles during activation were noted in the cortical area. Furthermore, the inflammatory processes were identified, morphologically manifested by the thickening of connective tissue in the lymph node capsule, dilacerations of the connective fibres and the presence of light acidophilic serous exudate with rare inflammatory cells (serous lymphoreticulitis).

Bionate Biocompatibility: In Vivo Study in Rabbits

“…We found no statistically significant differences between the control and implanted rabbits at 3 and 6 months post-operation ( Table 3 ), and the mean values for the different parameters fell within the published ranges. 36 At 3 months, the percentage of basophils (although not its count) increased slightly, while platelets decreased in both implanted and control rabbits. At 6 months, implanted rabbits showed slight eosinophilia but no statistically significant differences from the control group.…”

“…Next, we compared these values with reported hemogram reference values for rabbits. 36 We considered a p value of <0.05 as statistically significant.…”

“…First, we used Student’s t tests to find whether statistically significant differences existed between the implanted and control rabbits. Next, we compared these values with reported hemogram reference values for rabbits . We considered a p value of <0.05 as statistically significant.…”

Immune and Oxidative Response against Sonicated Antigen of Mycoplasma capricolum subspecies capripneumonia—A Causative Agent of Contagious Caprine Pleuropneumonia

“…The decrease in Hb and TEC in saponin-adjuvanted vaccinated groups compared to the montanide ISA 50-adjuvanted group may be due to the hemolytic nature of saponin, resulting in the reduction of hematological parameters or their severe immune or inflammatory nature, resulting in the alteration of Hb and TEC. Changes in Hb and TEC following vaccination in rabbits have been reported by Stancu et al [69]. Hemolytic activity of saponins is well known but this does not relate to adjuvant activity [29].…”

Haematological response in the treatment of naturally acquired ectoparasite infestations in rabbits

“…Data on haematological and serum biochemical parameters in healthy rabbits and rabbits with clinical diseases have increased, together with the increasing popularity of rabbits as pets (Hinton et al, 1982;Archetti et al, 2008;Özkan et al, 2012), but also in rabbit farmed for meat production (Belhassen et al, 2016;Stancu et al, 2017;Sun et al, 2018). The aim of the study was to investigate changes in haematology and selected biochemistry in the use of topical administration of selamectin for the treatment of naturally acquired ectoparasite infestations in farm rabbits.…”