Clinical Comparison of Grafts Stored in McCarey-Kaufman Medium at 4°C and in Corneal Organ Culture at 31°C

Rijneveld, Wilhelmina J.; Beekhuis, W. H.; Vanrij, G; Rinkelvandriel, B; Pels, Elisabeth

doi:10.1001/archopht.1992.01080140059027

Cited by 27 publications

(20 citation statements)

References 13 publications

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“…Storage-induced cell alterations have been extensively studied in the central cornea, the area of interest for corneal graft. Despite general satisfactory preservation of corneal viability, attested by constant good clinical results identical to 4 C short-term storage [52,53], death of central ECs increases dramatically during OC, with approximately 1% cell loss per day [54,55] compared with 0.6% per year throughout life [3,4]. However, OC-specific alterations of ECs in the extreme periphery have never been described.…”

Section: Discussionmentioning

confidence: 99%

Revisited Microanatomy of the Corneal Endothelial Periphery: New Evidence for Continuous Centripetal Migration of Endothelial Cells in Humans

et al. 2012

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The control of corneal transparency depends on the integrity of its endothelial monolayer, which is considered nonregenerative in adult humans. In pathological situations, endothelial cell (EC) loss, not offset by mitosis, can lead to irreversible corneal edema and blindness. However, the hypothesis of a slow, clinically insufficient regeneration starting from the corneal periphery remains debatable. The authors have re-evaluated the microanatomy of the endothelium in order to identify structures likely to support this homeostasis model. Whole endothelia of 88 human corneas (not stored, and stored in organ culture) with mean donor age of 80 6 12 years were analyzed using an original flatmounting technique. In 61% of corneas, cells located at the extreme periphery (last 200 lm of the endothelium) were organized in small clusters with two to three cell layers around Hassall-Henle bodies. In 68% of corneas, peripheral ECs formed centripetal rows 830 6 295 lm long, with Descemet membrane furrows visible by scanning electron microscopy. EC density was significantly higher in zones with cell rows. When immunostained, ECs in the extreme periphery exhibited lesser differentiation (ZO-1, Actin, Na/ K ATPase, CoxIV) than ECs in the center of the cornea but preferentially expressed stem cell markers (Nestin, Telomerase, and occasionally breast cancer resistance protein) and, in rare cases, the proliferation marker Ki67. Stored corneas had fewer cell clusters but more Ki67-positive ECs. We identified a novel anatomic organization in the periphery of the human corneal endothelium, suggesting a continuous slow centripetal migration, throughout life, of ECs from specific niches.

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Section: Discussionmentioning

confidence: 99%

Revisited Microanatomy of the Corneal Endothelial Periphery: New Evidence for Continuous Centripetal Migration of Endothelial Cells in Humans

et al. 2012

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“…La décompensation endothéliale primaire du greffon est une complication rare après kératoplastie transfixiante, surtout depuis l'apparition de nouvelles techniques de conservation des greffons [17]. Après kératoplastie lamellaire postérieure par DSAEK, Koenig et al [18] rapportent 12 % de décompensations primaires du greffon parmi leurs 36 premiers cas opérés, Gorovoy [10] 6 % de décom-pensations endothéliales primaires pour ses 16 premiers cas, et.…”

Section: Discussionunclassified

Complications après Descemet stripping automated endothelial keratoplasty : étude monocentrique sur 32 yeux

Ponchel

Arné

Malecaze

et al. 2009

Journal Français d'Ophtalmologie

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“…All consecutive patients with keratoconus on the waiting list for penetrating keratoplasty were selected for the study. After the preservation procedures, described elsewhere, 14 the paired corneas were transplanted into 2 age-matched recipients. All transplantations were performed by 2 surgeons (W.H.B.…”

Section: Methodsmentioning

confidence: 99%

“…The 5 excluded pairs included 1 because of a herpetic keratitis and graft rejection, 14 1 because of a cataract extraction, and 3 as a consequence of address changes.…”

Section: Methodsmentioning

confidence: 99%

“…Inspections 13.9 6 5.2 months postoperatively (1 year postoperatively) revealed no significant difference between the 2 groups in visual acuity, central corneal thickness, and central endothelial cell density (ECD). 14 At 166 6 7.8 months postoperatively (14 years postoperatively), 9 pairs of patients could be traced for follow-up. To our knowledge, this study is the only prospective, randomized study performed on a group of patients with the same diagnosis, comparing OC with MK, with a long-term followup.…”

mentioning

confidence: 99%

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Prospective Clinical Evaluation of McCarey-Kaufman and Organ Culture Cornea Preservation Media: 14-Year Follow-up

Rijneveld

Remeijer

Rij

et al. 2008

Cornea

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Clinical Comparison of Grafts Stored in McCarey-Kaufman Medium at 4°C and in Corneal Organ Culture at 31°C

Cited by 27 publications

References 13 publications

Revisited Microanatomy of the Corneal Endothelial Periphery: New Evidence for Continuous Centripetal Migration of Endothelial Cells in Humans

Revisited Microanatomy of the Corneal Endothelial Periphery: New Evidence for Continuous Centripetal Migration of Endothelial Cells in Humans

Complications après Descemet stripping automated endothelial keratoplasty : étude monocentrique sur 32 yeux

Prospective Clinical Evaluation of McCarey-Kaufman and Organ Culture Cornea Preservation Media: 14-Year Follow-up

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