Clinical consequences of resistant Candida infections in intensive care

Montravers, Philippe; Jabbour, Khalil

doi:10.1016/j.ijantimicag.2005.11.002

Cited by 15 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Candida albicans is one of the leading opportunistic fungal pathogens, which still causes serious mucosal and systemic infections among immunocompromised patients, including AIDS patients and patients undergoing transplantation or anticancer chemotherapy. Despite the severity of candidosis and reduced treatment possibilities emerging due to a rising number of resistant strains (Montravers & Jabbour, 2006), little is known about the virulence determinants of C. albicans or its interactions with the host organism. However, medical importance is by far not the only reason for a detailed investigation of C. albicans biology.…”

Section: Discussionmentioning

confidence: 99%

Ambiguous decoding of the CUG codon alters the functionality of the Candida albicans translation initiation factor 4E

Feketová

Mašek

Vopálenský

et al. 2010

FEMS Yeast Research

View full text Add to dashboard Cite

The eukaryotic translation initiation factor 4E is an essential and highly conserved protein. As a part of the translational machinery, it plays a key role in the recruitment of mRNA via binding to its m(7)GpppN 5' terminal cap structure. The opportunistic human pathogen Candida albicans is the only known eukaryotic organism with the ability to survive defects in mRNA capping, which suggests unique features of its eIF4E protein. Here, we provide the first experimental evidence of the function of the C. albicans putative gene orf19.7626 as an eIF4E protein. We also show that Ca4E(Leu116) and Ca4E(Ser116) protein variants, both of which occur naturally in C. albicans due to the ambiguous decoding of the CUG(116) codon, display different sensitivities to elevated temperature. Our results clearly point to the importance of the S4-H4 loop for the function of the Ca4E translation initiation factor, and suggest the possible regulatory role of the codon-reading ambiguity within this loop in C. albicans. We proved Saccharomyces cerevisiae as a useful tool organism for studies of C. albicans translation initiation apparatus.

show abstract

Section: Discussionmentioning

confidence: 99%

Ambiguous decoding of the CUG codon alters the functionality of the Candida albicans translation initiation factor 4E

Feketová

Mašek

Vopálenský

et al. 2010

FEMS Yeast Research

View full text Add to dashboard Cite

show abstract

“…[8] However, prolonged use of azoles has led to the development of drug resistance in C. albicans and other species. [69–13] Among the factors contributing to development of resistance to azoles, the selection of intrinsically less susceptible organisms, such as C. glabrata and Candida krusei , and the acquisition of resistance by previously susceptible strains of C. albicans following long-term azoles exposure have been documented. [1213] To manage the patients with candidiasis, antifungal susceptibility testing has become an important step in guiding physicians in the selection of proper antifungal therapy.…”

Section: Introductionmentioning

confidence: 99%

Determination of antifungal susceptibility patterns among the clinical isolates of Candida species

Zomorodian

Rahimi

Pakshir

et al. 2011

J Global Infect Dis

View full text Add to dashboard Cite

Context:Candida species are opportunistic yeasts that cause infections ranging from simple dermatosis to potentially life-threatening fungemia. The emergence of resistance to antifungal drugs has been increased in the past two decades.Aim:the present study we determined to find out the susceptibility profiles of clinical isolates of Candida species against four antifungal drugs, including amphotericin B, ketoconazole, fluconazole and itraconazole.Materials and Methods:Antifungal susceptibility testing of the yeasts was done in accordance with the proposed guidelines for antifungal disk diffusion susceptibility testing of yeasts based on the CLSI document M44-A.Results:A total of 206 yeast isolates were assessed. Among the evaluated Candida species, the highest rates of resistance to ketoconazole were seen in Candida glabrata (16.6%) and Candida albicans (3.2%). Susceptibility and intermediate response to fluconazole were seen in 96.6% and 3.4% of the Candida isolates, respectively. A total of 19 (9.2%) yeast isolates showed petite phenomenon including 11 C. glabrata, 3 C. albicans, 2 Candida dubliniensis and one isolate of each Candida krusei and Candida parapsilosis.Conclusion:The high number of petite mutation in the isolated yeasts should be seriously considered since it may be one of the reasons of antifungal treatment failure.

show abstract

“…The number of antifungal families available and their use in therapy is very limited (1)(2)(3)(4). Recently, a new family of specific fungal cell wall synthesis inhibitors has emerged as an alternative antifungal therapy and is gaining increasing relevance yearly (5)(6)(7).…”

mentioning

confidence: 99%

Differential Activities of Three Families of Specific β(1,3)Glucan Synthase Inhibitors in Wild-type and Resistant Strains of Fission Yeast

Martins¹,

Cortés²,

Muñoz³

et al. 2011

Journal of Biological Chemistry

View full text Add to dashboard Cite

Three specific ␤(1,3)glucan synthase (GS) inhibitor families, papulacandins, acidic terpenoids, and echinocandins, have been analyzed in Schizosaccharomyces pombe wild-type and papulacandin-resistant cells and GS activities. Papulacandin and enfumafungin produced similar in vivo effects, different from that of echinocandins. Also, papulacandin was the strongest in vitro GS inhibitor (IC 50 10 3 -10 4 -fold lower than with enfumafungin or pneumocandin), but caspofungin was by far the most efficient antifungal because of the following. 1) It was the only drug that affected resistant cells (minimal inhibitory concentration close to that of the wild type). 2) It was a strong inhibitor of wild-type GS (IC 50 close to that of papulacandin). 3) It was the best inhibitor of mutant GS. Moreover, caspofungin showed a special effect for two GS inhibition activities, of high and low affinity, separated by 2 log orders, with no increase in inhibition. pbr1-8 and pbr1-6 resistances are due to single substitutions in the essential Bgs4 GS, located close to the resistance hot spot 1 region described in Saccharomyces and Candida Fks mutants. Bgs4 pbr1-8 contains the E700V change, four residues N-terminal from hot spot 1 defining a larger resistance hot spot 1-1 of 13 amino acids. Bgs4 pbr1-6 contains the W760S substitution, defining a new resistance hot spot 1-2. We observed spontaneous revertants of the spherical pbr1-6 phenotype and found that an additional A914V change is involved in the recovery of the wild-type cell shape, but it maintains the resistance phenotype. A better understanding of the mechanism of action of the antifungals available should help to improve their activity and to identify new antifungal targets.

show abstract

Clinical consequences of resistant Candida infections in intensive care

Cited by 15 publications

References 49 publications

Ambiguous decoding of the CUG codon alters the functionality of the Candida albicans translation initiation factor 4E

Ambiguous decoding of the CUG codon alters the functionality of the Candida albicans translation initiation factor 4E

Determination of antifungal susceptibility patterns among the clinical isolates of Candida species

Differential Activities of Three Families of Specific β(1,3)Glucan Synthase Inhibitors in Wild-type and Resistant Strains of Fission Yeast

Contact Info

Product

Resources

About