Clinical Correlation of Adenoviral Load in the Respiratory Tract Measured by Digital PCR in Immunocompromised Children

Hijano, Diego R.; Ferrolino, Jose; Hidinger, Jennifer; Cardenas, Jessica N Brazelton de; Gu, Zheng; Suganda, Sri; Glasgow, Heather L.; Allison, Kim; PETERSON, M; Dallas, Ronald H.; Marón, Gabriela; Hayden, Randall T.

doi:10.1093/ofid/ofad030

Cited by 4 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, ADV has become one of the primary research topics in the field of epidemiology. 5,6 Various techniques are commonly employed for the detection of ADV, including virus isolation and culture, 7 polymerase chain reaction (PCR), 8 enzyme-linked immunosorbent assay (ELISA), 9 and others. However, these methods often have their limitations.…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Magnetic-Optical Triple-Mode Lateral Flow Immunoassay for Sensitive and Rapid Detection of Respiratory Adenovirus

Wang,

Li,

Liu

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

Human respiratory adenovirus (ADV) is a highly infectious respiratory virus with potential for pandemics. There are currently no specific drugs to treat ADV worldwide, so early rapid detection of ADV infection is essential. In this study, we developed an innovative magnetic-optical triple-mode lateral flow immunoassay (LFIA) using magnetic quantum dots as immunomarkers. This novel approach addresses the need for rapid and accurate ADV detection, allowing for multimodal quantitative/semiquantitative analysis of magnetic, fluorescent, and visible signals within a mere 15 min. The lower limit of detection (LOD) for magnetic, fluorescent, and visual signals was determined to be 5.6 × 10 3 , 1.2 × 10 3 , and 1.95 × 10 4 copies/mL, respectively. The detection range for ADV using this approach was 1.2 × 10 3 −5 × 10 7 copies/mL. Additionally, semiquantitative analysis, which is user-friendly and does not necessitate specialized equipment, was successfully implemented. Notably, seven respiratory viruses showed no crossreactivity with the generated LFIA test strips. The intrabatch repeatability exhibited a coefficient of variation (CV) of less than 5%, while the interbatch repeatability had a CV of less than 15%. Furthermore, recovery values ranged from 95% to 106.8% for samples analyzed concurrently with dual signals at the same spiking concentration. The assay developed in this study boasts a wide detection range and exceptional sensitivity and specificity. This technique is exceptionally well-suited for on-site rapid detection, with the potential for personal self-testing and early ADV infection diagnosis. Its versatility extends to a broad array of application scenarios.

show abstract

Section: ■ Introductionmentioning

confidence: 99%

“…The unpredictable outbreaks of ADV have placed significant strain on individuals’ lives and the public health systems dedicated to preventing epidemics in society. As a result, ADV has become one of the primary research topics in the field of epidemiology. , …”

Section: Introductionmentioning

confidence: 99%