Clinical Diagnosis by Laboratory Methods

Follett, Grace Parker; Todd, James C.; Sanford, Arthur

doi:10.2307/3411886

Cited by 11 publications

(9 citation statements)

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“…The hematocrit, hemoglobin level, red blood cell count (RBC), white blood cell count (WBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and differential white cell count were determined using standard methods (BAIN et al, 2011;TODD et al, 1979).…”

Section: Methodsmentioning

confidence: 99%

Hematological changes in Eleginops maclovinus during an experimental Caligus rogercresseyi infestation

Peña-Rehbein

Ruiz

Ortloff

et al. 2013

Rev. Bras. Parasitol. Vet.

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Eleginops maclovinus has been an important fishery resource in Chile since 1957. Caligus rogercresseyi is one of the most prevalent ectoparasite species found on E. maclovinus. Hematocrit, hemoglobin level, red blood cell count (RBC), white blood cell count (WBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and differential white blood cell count were determined before and after an experimental infestation with C. rogercresseyi. We found significant differences in the hemoglobin level, WBC, MCV, MCH, MCHC, hematocrit level and RBC between infested and uninfested fish. Furthermore correlations between number of C. rogercresseyi with hematocrit, MCHC, neutrophil, eosinophil and lymphocyte counts were found. Hematological reference ranges of E. maclovinus in captivity conditions were also established.

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Section: Methodsmentioning

confidence: 99%

Hematological changes in Eleginops maclovinus during an experimental Caligus rogercresseyi infestation

Peña-Rehbein

Ruiz

Ortloff

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…of dye per kgm. body weight with determinations 5 and 30 minutes after injection (5), alkaline phosphatase (6), serum bilirubin (7), serum cholesterol (8), cephalincholesterol flocculation (9), serum albumin and globulin by the salting-out method (10), occasional hippuric acid excretion tests; urine collected 1 hour after intravenous injection of 1.77 grams of sodium benzoate (11), nonprotein nitrogen (12), uric acid (13), and electrophoretic analysis of the blood serum (14). These and other studies were repeated at intervals during the course of the malarial fever and also during the period of antimalarial therapy and convalescence.…”

Section: Methodsmentioning

confidence: 99%

Significance of Cephalin-Cholesterol Flocculation Test in Malarial Fever 1

Guttman¹,

Potter²,

Hanger³

et al. 1945

J. Clin. Invest.

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“…In the presence of peroxidase, the hydrogen peroxide carries out the oxidative coupling of 3-5-dichloro-2-hydroxybenzenesulfonate (DHBS) and 4-aminoantipyrine to form a quinoneimine derivative of red colour. The colour intensity is directly related to the UA concentration and is measured photometrically at 520 nm [20].…”

Section: Comparison Methodsmentioning

confidence: 99%

Automatic Determination of Uric Acid in Urine in a FIA System with a Tubular Amperometric Detector

Garcia

Lima

Silva

et al. 2004

Port. Electrochim. Acta

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This paper describes the use of a FIA system for the automatic determination of uric acid in urine, using a tubular amperometric detector. In this system, glassy carbon electrodes of tubular configuration were activated by successive cyclical scans from -0.30 to 2.00 V versus Ag/AgCl reference electrode in 0.50 mol.L -1 sulphuric acid solution. Samples were diluted in supporting electrolyte before analysis and no other pre-treatment was employed. Such dilution enabled the matrix effects to be reduced and therefore improving the sensitivity provided by the activation step that allows the uric acid (UA) to be detected at lower concentration levels after dilution. Uric acid determination was performed in a single channel FIA manifold, which provided reproducibility of sample transport to the detector and enabled a sampling rate of 120 samples per hour to be achieved. The tubular configuration of the cell offered robustness to the system since it was rigidly fixed to the manifold, allowing the manifold to be generalised for routine analysis application. The results obtained with the FIA system for UA determination in urine were compared with those from the enzymatic method used in clinical analysis laboratories. No statistical difference between methods was found at the 95% confidence level. Relative deviations between both methods were within ± 4% and the proposed system showed good repeatability (about 3%, n = 10).

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Clinical Diagnosis by Laboratory Methods

Cited by 11 publications

References 0 publications

Hematological changes in Eleginops maclovinus during an experimental Caligus rogercresseyi infestation

Hematological changes in Eleginops maclovinus during an experimental Caligus rogercresseyi infestation

Significance of Cephalin-Cholesterol Flocculation Test in Malarial Fever 1

Automatic Determination of Uric Acid in Urine in a FIA System with a Tubular Amperometric Detector

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