Clinical efficacy of adipocyte-derived stem cells conditioned media combined with micro-injury in refractory patch of alopecia areata

Lee, Seon Bok; Shin, Hyun-Tae; Byun, Ji Won; Shin, Jeonghyun; Choi, Gwang Seong

doi:10.1007/s00403-021-02252-9

Cited by 10 publications

(13 citation statements)

References 18 publications

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“…Several reports have demonstrated that fractional laser treatment effectively induces hair regrowth in combination with other factors, including platelet-rich plasma, 21 hair growth factors, 39 and MSCconditioned medium. 40 Similar results were reported with 308 nm excimer laser/light treatment for alopecia areata (AA). 41,42 These positive effects may be produced not only by their ability to penetrate the skin barrier, 20 but also by the inflammation and mechano-physical signals induced by fractional laser irradiation.…”

Section: Discussionsupporting

confidence: 70%

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Single‐cell transcriptome analysis of fractional CO₂ laser efficiency in treating a mouse model of alopecia

Hasegawa

Fujimoto²,

Mita

et al. 2022

Lasers Surg Med

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Objectives Hair loss, including alopecia, is a common dermatological issue worldwide. At present, the application of fractional carbon dioxide (CO2) laser in the treatment of alopecia has been documented; however, the results vary between reports. These varying results may be due to the limited knowledge of cellular action in laser‐irradiated skin. The objective of this study was to investigate the molecular and cellular mechanisms of laser treatment under effective conditions for hair cycle initiation. Methods A fractional CO2 laser was applied and optimized to initiate the hair cycle in a mouse model of alopecia. Several cellular markers were analyzed in the irradiated skin using immunofluorescence staining. Cellular populations and their comprehensive gene expression were analyzed using single‐cell RNA sequencing and bioinformatics. Results The effective irradiation condition for initiating the hair cycle was found to be 15 mJ energy/spot, which generates approximately 500 μm depth columns, but does not penetrate the dermis, only reaching approximately 1 spot/mm2. The proportion of macrophage clusters significantly increased upon irradiation, whereas the proportion of fibroblast clusters decreased. The macrophages strongly expressed C–C chemokine receptor type 2 (Ccr2), which is known to be a key signal for injury‐induced hair growth. Conclusions We found that fractional CO2 laser irradiation recruited Ccr2 positive macrophages, and induced hair regrowth in a mouse alopecia model. These findings may contribute to the development of stable and effective fractional laser irradiation conditions for human alopecia treatment.

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Section: Discussionsupporting

confidence: 70%

“…Several reports have demonstrated that fractional laser treatment effectively induces hair regrowth in combination with other factors, including platelet‐rich plasma, 21 hair growth factors, 39 and MSC‐conditioned medium 40 . Similar results were reported with 308 nm excimer laser/light treatment for alopecia areata (AA) 41,42 .…”

Section: Discussionsupporting

confidence: 62%

Single‐cell transcriptome analysis of fractional CO₂ laser efficiency in treating a mouse model of alopecia

Hasegawa

Fujimoto²,

Mita

et al. 2022

Lasers Surg Med

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“…4 In another study of 14 patients with refractory AA receiving combined treatment with ADSCs and either 10,600 nm CO 2 fractional laser or microneedling, twothirds of patients demonstrated greater than 50% hair regrowth, and almost half experienced full recovery. 5 Finally, a recent systematic review including 188 patients with non-scarring alopecia found hair density improvement with SVF compared with control or baseline in all studies. 6 No severe adverse events were reported.…”

Section: Regenerative Therapies In Alopecia Areatamentioning

confidence: 97%

Regenerative therapies in alopecia areata

Christensen

Anvery

Dirr

2022

J of Cosmetic Dermatology

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“…Recent studies have reported that EVs are associated with skin physiology in the cutaneous microenvironment. Adipocyte-derived stem cell–conditioned media can activate hair growth, and microinjury using a fractional laser or microneedling can also induce wound healing and hair regeneration ( Lee et al, 2021 ). The significantly reduction of mRNA expression of various pro-inflammatory cytokines in alleviate atopic dermatitis skin lesions on mice model by the ADSC-derived exosomes ( Cho et al, 2018 ) provides the potential cell-free therapy of ADSC-derived exosomes in other immunological skin disease, such as vitiligo.…”

Section: Immunomodulatory Effects Of Mscs and Their Extracellular Vesiclesmentioning

confidence: 99%

Vitiligo: An Autoimmune Skin Disease and its Immunomodulatory Therapeutic Intervention

Chang

Lee

Kuo

et al. 2021

Front. Cell Dev. Biol.

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Vitiligo is a chronic autoimmune depigmenting skin disorder characterized by patches of the skin losing functional melanocytes. Multiple combinatorial factors are involved in disease development, among which immune T cells play a prominent role. The immune cells implicated in melanocyte destruction through adaptive immunity include CD8+ cytotoxic T cells and regulatory T cells, and aberrantly activated skin-resident memory T cells also play a role in melanocyte destruction. Over the past several years, major progress in understanding vitiligo pathogenesis has led to the development of targeted therapies. Janus kinase (JAK) inhibitors, which share the similar mechanism that autoactivates CD8+ T cells in chronic inflammatory diseases, have been reported to have therapeutic significance in vitiligo. Recently, immunomodulatory therapeutic interventions in vitiligo have been emerging. Mesenchymal stem cells (MSCs) regulate cytokine secretion and the balance of T-cell subsets, which makes them a promising cell-based treatment option for autoimmune diseases. The induction of MSC-mediated immunomodulation is complicated and occurs by contact-dependent mechanisms and soluble extracellular vesicle (EV) mediators. EVs released from MSCs contain various growth factors and cytokines with anti-inflammatory effects in the skin immune response. Here, we summarize and discuss the progress to date in targeted therapies that immunomodulate the niche environment of vitiligo, from the clinical trial of JAK inhibitors to the potential of MSCs and MSC-EVs. The available information was collected to highlight the need for further research into the treatment of vitiligo.

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Clinical efficacy of adipocyte-derived stem cells conditioned media combined with micro-injury in refractory patch of alopecia areata

Cited by 10 publications

References 18 publications

Single‐cell transcriptome analysis of fractional CO₂ laser efficiency in treating a mouse model of alopecia

Single‐cell transcriptome analysis of fractional CO₂ laser efficiency in treating a mouse model of alopecia

Regenerative therapies in alopecia areata

Vitiligo: An Autoimmune Skin Disease and its Immunomodulatory Therapeutic Intervention

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Clinical efficacy of adipocyte-derived stem cells conditioned media combined with micro-injury in refractory patch of alopecia areata

Cited by 10 publications

References 18 publications

Single‐cell transcriptome analysis of fractional CO2 laser efficiency in treating a mouse model of alopecia

Single‐cell transcriptome analysis of fractional CO2 laser efficiency in treating a mouse model of alopecia

Regenerative therapies in alopecia areata

Vitiligo: An Autoimmune Skin Disease and its Immunomodulatory Therapeutic Intervention

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Product

Resources

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Single‐cell transcriptome analysis of fractional CO₂ laser efficiency in treating a mouse model of alopecia

Single‐cell transcriptome analysis of fractional CO₂ laser efficiency in treating a mouse model of alopecia