Background: The therapeutic effects of music therapy on improving negative emotions and reducing pain are increasingly acknowledged. However, limited clinical research exists on its use in patients post-laparoscopic ovarian cystectomy (LOC). Materials and Methods: A total of 147 patients undergoing LOC at our hospital were evaluated. Patients were divided based on the implementation time of music therapy: group A (72 patients, October 2020 to October 2021) received standard clinical treatment, while group B (75 patients, November 2021 to November 2022) received music therapy alongside routine care. The baseline data of patients and the scores of the Short-Form McGill Pain Questionnaire (SF-MPQ), Perceived Stress Scale (PSS), Beck Anxiety Inventory (BAI), and Patient Satisfaction Questionnaire were collected. Pain and psychological stress levels were compared on the first postoperative day and at discharge to assess the clinical value of each treatment approach. Results: Group B exhibited significantly lower PSS, SF-MPQ, and BAI scores (P < 0.001 for all) and higher overall satisfaction at discharge (P < 0.001). These findings suggest that music therapy can reduce psychological stress, decrease pain levels, and improve mood in patients undergoing LOC. Conclusion: This study demonstrates that music therapy positively rehabilitates patients after LOC, offering new insights for future clinical treatment strategies.