Clinical, epidemiological, and microbiological features of Vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in Israel

Bisharat, Naiel; Agmon, Vered; Finkelstein, Renato; Raz, Raul; Ben-Dror, G; Lerner, Larisa; Soboh, Soboh; Colodner, Raul; Cameron, Daniel N.; Wykstra, David L; Swerdlow, David L.; Farmer, J. J.

doi:10.1016/s0140-6736(99)02471-x

Cited by 309 publications

(251 citation statements)

References 22 publications

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“…The pilF assay thus contrasts with other targets used to identify pathogenic strains of V. vulnificus, such as 16S rRNA (Aznar et al, 1994;Nilsson et al, 2003;Vickery et al, 2007) and vcgC polymorphisms (Rosche et al, 2005;Warner and Oliver, 2008;Baker-Austin et al, 2010b), which have shown limited usefulness in detecting biotype 2 and biotype 3 V. vulnificus strains potentially dangerous to human health (Roig et al, 2010;Sanjuan et al, 2009). Both biotype 2 (serovar E and serovar I) and biotype 3 V. vulnificus strains represent important human pathogens (Amaro and Biosca, 1996;Bisharat et al, 1999), and the ability to identify potentially virulent strains rapidly is of paramount importance. We found that a small number of non-pathogenic V. vulnificus strains (i.e.…”

Section: Resultsmentioning

confidence: 99%

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pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance

Baker‐Austin¹,

Lemm²,

Hartnell³

et al. 2012

Food Microbiology

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a b s t r a c tThe Gram-negative bacterium Vibrio vulnificus is a common inhabitant of estuarine environments. Globally, V. vulnificus is a significant foodborne pathogen capable of causing necrotizing wound infections and primary septicemia, and is a leading cause of seafood-related mortality. Unfortunately, molecular methods for the detection and enumeration of pathogenic V. vulnificus are hampered by the genetically diverse nature of this pathogen, the range of different biotypes capable of infecting humans and aquatic animals, and the fact that V. vulnificus contains pathogenic as well as non-pathogenic variants. Here we report an alternative approach utilizing the development of a real-time PCR assay for the detection of pathogenic V. vulnificus strains based on a polymorphism in pilF, a gene previously indicated to be associated with human pathogenicity. Compared to human serum reactivity, the real-time PCR assay successfully detected pathogenic strains in 46 out of 47 analysed V. vulnificus isolates (97.9%). The method is also rapid, sensitive, and more importantly can be reliably utilised on biotype 2 and 3 strains, unlike other current methods for V. vulnificus virulence differentiation.Crown

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Section: Resultsmentioning

confidence: 99%

“…vulnificus is currently subdivided into three biotypes based on genetic, biochemical and serological features, as well as host range (Bisharat et al, 1999;Tison et al, 1982). Biotype 1 strains are human pathogens and are responsible for the vast majority of V. vulnificus infections reported worldwide.…”

Section: Introductionmentioning

confidence: 99%

pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance

Baker‐Austin¹,

Lemm²,

Hartnell³

et al. 2012

Food Microbiology

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show abstract

“…Human vibriosis occurs after eating contaminated seafood or exposing open wounds to seawater (Veenstra et al, 1992;Strom & Paranjpye, 2000), and fish vibriosis after immersion in contaminated water or contact with diseased animals or carriers (Amaro et al, 1995;Marco-Noales et al, 2001;Valiente & Amaro, 2006). The species has been subdivided into three biotypes on the basis of differences in biochemical properties, such as indole production and cellobiose fermentation, as well as serological and genetic traits and host range (Tison et al, 1982;Bisharat et al, 1999Bisharat et al, , 2007. Biotypes 1 (BT1) and 3 (BT3) are considered human pathogens and biotype 2 (BT2) pathogens of aquatic animals (Tison et al, 1982;Song et al, 1990;Biosca et al, 1996Biosca et al, , 1997Bisharat et al, 1999;Fouz et al, 2002Fouz et al, , 2006aFouz & Amaro, 2003) in spite of the fact that several human cases of wound infection and sepsis due to BT2 have occurred worldwide (Veenstra et al, 1992;Dalsgaard et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and genotypic characterization of a new fish-virulent Vibrio vulnificus serovar that lacks potential to infect humans

2007

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Vibrio vulnificus is a bacterial species that is virulent for humans and fish. Human isolates are classified into biotypes 1 and 3 (BT1 and BT3) and fish isolates into biotype 2 (BT2). However, a few human infections caused by BT2 isolates have been reported worldwide (zoonosis). These BT2 human isolates belong to serovar E (SerE), which is also present in diseased fish. The aim of the present work was to characterize a new BT2 serovar [serovar A (SerA)], which emerged in the European fish-farming industry in 2000, by means of phenotypic, serological and genetic [plasmid profiling, ribotyping and random amplified polymorphic DNA (RAPD)] methodologies. The results confirmed that SerA constitutes a homogeneous O-serogroup within the species that shares plasmidic information with SerE. Like SerE, this new serogroup was resistant to fresh fish serum, as well as being highly virulent for fish. In contrast, it was sensitive to human serum and avirulent for mice, even after pretreatment with iron. The two serovars presented different biochemical profiles as well as specific patterns by ribotyping and RAPD analysis. In conclusion, SerA seems to constitute a different clonal group that has recently emerged within the species V. vulnificus, with pathogenic potential for fish but not for humans.

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“…Die Infektion erfolgt über den Kontakt offener Wunden mit erregerhaltigem Wasser (zum Beispiel beim Waten oder Baden), oder, wie in den USA [18] und Israel [19] beschrieben, beim Verarbeiten roher Fische oder Meeresfrüchte. V. parahaemolyticus verursacht vorrangig Gastroenteritiden, wohingegen V. vulnificus und V. alginolyticus vor allem Wundinfektionen/Septikämien hervorrufen.…”

Section: Epidemiologie Von Wundinfektionen Und Septikämien Durch Vibrunclassified

Vibrio-Infektionen durch Lebensmittel und Meerwasser

Alter

Appel

Bartelt

et al. 2011

Bundesgesundheitsbl.

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Clinical, epidemiological, and microbiological features of Vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in Israel

Cited by 309 publications

References 22 publications

pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance

pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance

Phenotypic and genotypic characterization of a new fish-virulent Vibrio vulnificus serovar that lacks potential to infect humans

Vibrio-Infektionen durch Lebensmittel und Meerwasser

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