2022
DOI: 10.1128/spectrum.00259-22
|View full text |Cite
|
Sign up to set email alerts
|

Clinical Evaluation of a Line-Probe Assay for Tuberculosis Detection and Drug-Resistance Prediction in Namibia

Abstract: Mycobacterium tuberculosis ( Mtb ) drug-resistance detection is crucial for successful control of tuberculosis. Line-probe assays (LPA) are frequently used to detect resistance to rifampin, isoniazid, fluoroquinolones (FQs), and second-line injectables (SLIs).

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
2
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
4

Relationship

0
4

Authors

Journals

citations
Cited by 4 publications
(2 citation statements)
references
References 11 publications
0
2
0
Order By: Relevance
“…The second-line LPA tests for FLQ resistance from mutations affecting codons 90, 91 and 94 of the gyrA gene. Resistance to injectable anti-TB drugs involves mutations in the rrs gene on codons 1401 and 1402, associated with resistance to AMK and KAN, and mutations on codon 1484, associated with resistance to all three drugs (AMK, KAN and CAP) [ 6 ].…”
Section: Introductionmentioning
confidence: 99%
“…The second-line LPA tests for FLQ resistance from mutations affecting codons 90, 91 and 94 of the gyrA gene. Resistance to injectable anti-TB drugs involves mutations in the rrs gene on codons 1401 and 1402, associated with resistance to AMK and KAN, and mutations on codon 1484, associated with resistance to all three drugs (AMK, KAN and CAP) [ 6 ].…”
Section: Introductionmentioning
confidence: 99%
“…Line probe assays (LPAs), which are based on polymerase chain reaction (PCR), are mainly used to detect drug‐resistant tuberculosis, especially rifampicin (RIF)‐resistant tuberculosis, isoniazid (INH)‐resistant tuberculosis and multidrug‐resistant (MDR) tuberculosis. However, the utility of this method in patients with HIV/TB co‐infection is limited for the following reasons: (a) LPAs requires a sufficient amount of bacillary DNA load, but the acid‐fast bacilli (AFB) load in patients with HIV/TB co‐infection is low [22]; (b) nearly 24%–61% [23] of patients with HIV/TB co‐infection have AFB smear‐negative sputum, and the sensitivity of LPAs for detecting AFB in smear‐negative samples is lower (44.1%) than it is in AFB smear‐positive samples (94.6%) [24]. Therefore, LPAs was recommended only for detecting drug‐resistant TB, and not AFB smear‐negative TB, by the WHO in 2008 (Figure 3) [14].…”
Section: Recent Advances In Molecular Diagnostic Methods For Tubercul...mentioning
confidence: 99%