Clinical evaluation of a multiparametric<scp>ELISA</scp>as a rapid tool for routinely diagnosing IgG‐mediated autoimmune blistering dermatoses in ethnic Slavs

Gornowicz‐Porowska, Justyna; Seraszek‐Jaros, Agnieszka; Bowszyc‐Dmochowska, Monika; Bartkiewicz, Paweł; Kaczmarek, Elżbieta; Dmochowski, Marian

doi:10.1002/jcla.22336

Cited by 9 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our case was diagnosed with a combination of imaging and biochemical-molecular techniques. The DIF visualised with the blue light-emitting diode technology-operated microscopy for patterning of IgG4 and IgG1 deposits along the DEJ in perilesional tissue combined with the multiplex ELISA detecting serum IgG antibodies to BP180 and/or BP230 is an approach advocated by us for routine differential laboratory diagnostics of BP in our native population ( 11 , 12 ). Usually, we detect IgG4, but not IgG1 deposits with DIF in BP, nevertheless in our case IgG1, but not IgG4, deposits were detected ( 13 ).…”

Section: Discussionmentioning

confidence: 99%

Case Report: Infantile Bullous Pemphigoid: Triggering by COVID-19 Is Speculative

et al. 2021

Self Cite

View full text Add to dashboard Cite

Bullous pemphigoid (BP) is a cutaneous disease triggered by numerous stimuli, where genetic milieu-influenced autoimmunity to hemidesmosomal proteins, namely, BP180 and/or BP230 initiate an inflammation leading to dermal-epidermal junction (DEJ) enzymatic pathological remodelling. Here, to the best of our knowledge, we present the first case of an infantile BP apparently triggered by COVID-19. BP should be included in differential diagnosis of infantile rashes showing blisters or vesicles or both as well as their prodromal and evolutionary lesions. Possible triggers, such as coronavirus disease 2019 (COVID-19), of BP in infancy should be identified and properly dealt with.

show abstract

Section: Discussionmentioning

confidence: 99%

Case Report: Infantile Bullous Pemphigoid: Triggering by COVID-19 Is Speculative

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Still, there are cases of malignancy-associated PV without laboratory features of PNP. A multiplex ELISA containing envoplakin combined with a clinical examination and a DIF should be helpful for routine laboratory diagnostics and differentiation of PNP [ 22 ].…”

Section: Case Report: Ocular Involvementmentioning

confidence: 99%

Sacral Dimple, Conjunctiva, and Nipple as Less Obvious Pemphigus Vulgaris Locations around Natural Body Orifices: A Report of Three Cases

Jałowska

Gornowicz‐Porowska

Bowszyc‐Dmochowska

et al. 2022

JCM

Self Cite

View full text Add to dashboard Cite

In this paper, we present our own clinical-laboratory experience concerning three less obvious presentations of pemphigus vulgaris (PV) and discuss the pertinent literature. The involvement of the sacral dimple reported here for the first time, as well as the nipple and the eyes, could initially be misleading clinically. These less stereotypical localizations may occur due to the transition of different epithelia, each with varying levels of cadherin (desmoglein, desmocollin) and thus altered sensitivity to mechanical stress. The role of dermatologists who have experience in treating autoimmune blistering dermatoses is fundamental for identifying promptly the initial and exacerbating PV lesions in such unusual locations.

show abstract

“…Recent studies showed that the sensitivity and specificity of BIOCHIP and ELISA in pemphigus diagnostics and type differentiation are comparable [ 11 ]. Both BIOCHIP and the recently used multiparametric ELISA allow for quick, one-step and simultaneous identification of autoantibodies directed against various antigens in several sera, which is particularly useful in patients with an unusual clinical picture and convenient for use in small laboratories [ 12 , 13 ]. Routinely, immunoblot and immunoprecipitation are rarely used [ 14 ].…”

Section: Pemphigus Groupmentioning

confidence: 99%

Diagnostics of autoimmune blistering disorders: an experience of a single tertiary referral centre

Adaszewska¹,

Woźniak²,

Kalińska-Bienias³

et al. 2022

pdia

View full text Add to dashboard Cite

Autoimmune blistering disorders (AIBD) include a heterogeneous group of diseases characterized by the presence of autoantibodies against the structural antigens of the skin and mucous membranes. The gold standard of AIBD diagnostics is the detection of in vivo bound IgG/IgA and/or complement component 3 in the direct immunofluorescence of a perilesional biopsy. Various immunological techniques such as indirect immunofluorescence of different tissue substrates including monkey oesophagus, salt split skin, recombinant proteins of epidermis and basement membrane zone as well as ELISA systems and immunoblotting are used to characterize target antigens. Proper and early diagnosis is crucial for both treatment and prognosis since some AIBD may be associated with a malignant neoplasm.

show abstract

Clinical evaluation of a multiparametricELISAas a rapid tool for routinely diagnosing IgG‐mediated autoimmune blistering dermatoses in ethnic Slavs

Abstract: Multivariant ELISA test combined with clinical examinations and DIF is recommended as a minimal approach to diagnosing ABDs in ethnic Slavs.

Cited by 9 publications

References 19 publications

Case Report: Infantile Bullous Pemphigoid: Triggering by COVID-19 Is Speculative

Case Report: Infantile Bullous Pemphigoid: Triggering by COVID-19 Is Speculative

Sacral Dimple, Conjunctiva, and Nipple as Less Obvious Pemphigus Vulgaris Locations around Natural Body Orifices: A Report of Three Cases

Diagnostics of autoimmune blistering disorders: an experience of a single tertiary referral centre

Contact Info

Product

Resources

About