Clinical Evaluation of a Novel Urine Collection Kit Using Filter Paper in Neonates: An Observational Study

Nagano, Nobuhiko; Imaizumi, Takayuki; T, Akimoto; Hijikata, Makoto; Aoki, Ryoji; Seimiya, Ayako; Okahashi, Aya; Kawakami, Katsuhiro; Komatsu, Akira; Kawana, Kei; Morioka, Ichiro

doi:10.3390/children8070561

Cited by 3 publications

(7 citation statements)

References 21 publications

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“…These results were in accordance with the Glc 4 reference range described by An et al, 2000 (ref. 33) and Piraud et al, 2020, 6 as well as our previous results using liquid urine, 20 evidencing the reliability of the proposed method to quantify the Glc 4 directly in urine samples on lter paper.…”

Section: Lc-hrms Determinationsupporting

confidence: 78%

“…An inclusion list of m / z values for the Glc 4 ( m / z 665.21), acarbose ( m / z 644.24) and creatinine ( m / z 112.05) was used for the PRM experiment. 20…”

Section: Methodsmentioning

confidence: 99%

“…A previous LC-HRMS analysis of these samples can be found elsewhere. 20 An amount of 1.0 mL of urine was transferred to a piece of lter paper (Whatman 903) cut to 2.5 × 5.0 cm that was placed inside a plastic box (width 2.5 cm × length 5.0 cm). The lter paper was le inside the open box at room temperature (22 °C ± 0.3) for 24 h to ensure complete dryness.…”

Section: Collection and Extraction Of Urine Samplesmentioning

confidence: 99%

“…16 The use of lter paper combined or not with the liquid chromatography-mass spectrometry (LC-MS) technique has been successfully applied for urine screening of different inherited disorders, such as mucopolysaccharidoses, 15 Fabry disease, 17 Gaucher disease, 15 ornithine transcarbamylase deciency, 18 triple H syndrome, 18 Pompe disease, 13 and other clinical evaluations. 20 Although many studies have reported urine specimens dried on lter paper, to our knowledge, no one has proposed a urine collection kit combined with an analysis method for monitoring Glc 4 in Pompe disease.…”

Section: Introductionmentioning

confidence: 99%

“…Samples were analyzed in the scan range of m/z 100 to 1000 at a resolution of 70 000 FWHM (full width at half maximum) followed by a combination of data-dependent MS/ MS analysis (ddMS2 top3 experiment, normalized collision energy (NCE) 25%), and parallel reaction monitoring (PRM, NCE 12%) analysis, both using the resolution of 17 500 FWHM. An inclusion list of m/z values for the Glc 4 (m/z 665.21), acarbose (m/z 644.24) and creatinine (m/z 112.05) was used for the PRM experiment 20.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Development of a kit for urine collection on filter paper as an alternative for Pompe disease screening and monitoring by LC-HRMS

Ribeiro¹,

Scalco²,

Garrett³

et al. 2023

Anal. Methods

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Section: Lc-hrms Determinationsupporting

confidence: 78%

“…An inclusion list of m / z values for the Glc 4 ( m / z 665.21), acarbose ( m / z 644.24) and creatinine ( m / z 112.05) was used for the PRM experiment. 20…”

Section: Methodsmentioning

confidence: 99%

Section: Collection and Extraction Of Urine Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Development of a kit for urine collection on filter paper as an alternative for Pompe disease screening and monitoring by LC-HRMS

Ribeiro¹,

Scalco²,

Garrett³

et al. 2023

Anal. Methods

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Breast Milk and Saliva for Postnatal Cyto†megalovirus Screening among Very Low Birth Weight Infants

Mukhopadhyay

Itell

Hartman

et al. 2022

Pediatric Infectious Disease Journal

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Background: The optimal approach to managing postnatal cytomegalovirus disease (pCMV) among very low birth weight (VLBW) infants remains unknown. Methods to facilitate screening are needed. Objective: Determine whether mother’s milk and infant saliva can be used to reliably identify maternal cytomegalovirus (CMV) serostatus and detect infant pCMV acquisition. Methods: This was a single-center, prospective cohort study of VLBW infants, and their mothers, born between 2017 and 2020. Maternal milk samples were tested for CMV immunoglobulin G (IgG) using a CMV glycoprotein B binding enzyme-linked immunosorbent assay and the results were compared with maternal serum CMV IgG results. Biweekly paired saliva and urine samples were collected from infants born to mothers with positive or unknown CMV serostatus. Saliva samples were tested for CMV DNA by quantitative real-time polymerase chain reaction (PCR) and compared with urine CMV qualitative PCR results obtained from a clinical laboratory. Results: Among 108 infants without congenital CMV included in the study, 10 (9%) acquired pCMV. Both milk and blood CMV serology results were available for 70 mothers. Maternal milk antibody testing had a sensitivity of 97.2% (95% CI: 85.5–99.9%) and specificity of 91.2% (95% CI: 76.3–98.1%) in establishing CMV serostatus. Paired serially collected saliva and urine samples (n = 203) were available for 66 infants. Saliva PCR had a sensitivity of 30.0% (95% CI: 6.7–65.2%) and specificity of 92.7% (95% CI: 88.1–96.0%) in detecting pCMV acquisition. Conclusions: Maternal breast milk is a reliable alternative sample to determine CMV serostatus. Serial testing of infant saliva was not adequately sensitive for identifying pCMV acquisition in preterm infants.

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Droplet Digital PCR (ddPCR) Does Not Enhance the Sensitivity of Detection of Cytomegalovirus (CMV) DNA in Newborn Dried Blood Spots Evaluated in the Context of Newborn Congenital CMV (cCMV) Screening

Hernandez-Alvarado,

Bierle,

Schleiss

2023

IJNS

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Congenital cytomegalovirus (cCMV) infection is a leading cause of sensorineural hearing loss (SNHL) and neurodevelopmental disabilities in children worldwide. Some regions in the United States and Canada have implemented universal newborn screening for cCMV, which requires molecular diagnostic technologies for identifying cCMV, such as PCR testing of newborn dried blood spots (DBS). This study aimed to evaluate the sensitivity of droplet digital PCR (ddPCR) compared to quantitative real-time PCR to detect CMV DNA in newborn DBS. The limit of detection of various ddPCR primer/probe combinations (singleplex UL55-HEX, singleplex UL83-FAM, and multiplex UL55-HEX/UL83-FAM) was evaluated using the National Institute of Standards and Technology’s (NIST) CMV quantitative standard. Singleplex UL55-HEX ddPCR exhibited the lowest limit of detection among the primer/probe combinations tested for ddPCR. UL55 ddPCR was then compared to real-time PCR in 49 infants with confirmed cCMV identified through newborn screening for CMV in saliva swabs and confirmed by a urine test. The results showed that ddPCR was only positive for 59% (29 out of 49) of the cCMV infants, while real-time PCR was positive for 80% (39 out of 49). Due to its lower sensitivity and throughput, ddPCR may not be suitable for cCMV newborn screening.

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Clinical Evaluation of a Novel Urine Collection Kit Using Filter Paper in Neonates: An Observational Study

Cited by 3 publications

References 21 publications

Development of a kit for urine collection on filter paper as an alternative for Pompe disease screening and monitoring by LC-HRMS

Development of a kit for urine collection on filter paper as an alternative for Pompe disease screening and monitoring by LC-HRMS

Breast Milk and Saliva for Postnatal Cyto†megalovirus Screening among Very Low Birth Weight Infants

Droplet Digital PCR (ddPCR) Does Not Enhance the Sensitivity of Detection of Cytomegalovirus (CMV) DNA in Newborn Dried Blood Spots Evaluated in the Context of Newborn Congenital CMV (cCMV) Screening

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