Clinical evaluation of a simple image cytometer for CD4 enumeration on HIV‐infected patients

Li, X.; Breukers, Christian; Ymeti, Aurel; Pattanapanyasat, Kovit; Sukapirom, Kasama; Terstappen, Leon W.M.M.; Greve, Jan

doi:10.1002/cyto.b.20488

Cited by 14 publications

(6 citation statements)

References 20 publications

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“…Accordingly, there have been large efforts over the last decade 8 to miniaturize flow cytometers [9][10][11][12][13] or to develop alternative portable tests that can be carried out with minimal training, 7,14 for example LabNow, 15 PIMA (Alere), 16 Daktari, [17][18][19] Zyomyx. 20 Earlier, we developed a single platform image cytometer 21 that simplified the instrumentation and provided very promising results, [22][23][24] but still required precision for the sample preparation, which frequently cannot be met in the field by less experienced operators. Automated sample preparation is possible (e.g.…”

Section: Introductionmentioning

confidence: 99%

On-chip sample preparation by controlled release of antibodies for simple CD4 counting

Beck¹,

Brockhuis²,

Velde³

et al. 2012

Lab Chip

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We present a simple system for CD4 and CD8 counting for point-of-care HIV staging in low-resource settings. Automatic sample preparation is achieved through a dried reagent coating inside a thin (26 μm) counting chamber, allowing the delayed release of fluorochrome conjugated monoclonal antibodies after the filling of the chamber with whole blood by capillary flow. A custom-built image cytometer is used to capture fluorescence images representing more than 1 μl of blood. The thin layer of blood in combination with the large image area allows the use of whole blood from a finger prick without the need for dilution, lysis or cell enrichment. Automatic cell counting of CD4(+) and CD8(+) T-lymphocytes correlates well with results obtained by flow cytometry.

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Section: Introductionmentioning

confidence: 99%

On-chip sample preparation by controlled release of antibodies for simple CD4 counting

Beck¹,

Brockhuis²,

Velde³

et al. 2012

Lab Chip

Self Cite

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“…There have also been significant efforts by many different groups in the development of "portable," lowcost flow cytometry systems [25][26][27][28][29]. These and other studies have demonstrated the feasibility of producing at least limited numbers of working prototypes of such instruments, but none have yet been developed into fully integrated test platforms that could be considered suitable for field evaluation or POC use.…”

Section: Measurement Of Cd4 T Cells and %Cd4mentioning

confidence: 97%

Point-of-Care Testing

2010

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The availability of rapid, point-of-care (POC) tests has significantly expanded the capacity of both developed and resource-constrained countries (RCCs) to diagnose HIV, with immunochromatographic tests most commonly used in these settings. This has been especially important in programs for prevention of mother-to-child transmission, in both RCCs and the developed world. However, suitable POC tests are not yet commercially available for diagnosis of neonatal HIV, where persistence of maternal antibody in the infant precludes the use of current antibody tests during the first 12 to 18 months. In addition, measurement of CD4+ T cells, CD4%, and HIV viral load still relies on sophisticated laboratory infrastructure, constraining the delivery of appropriate care to many HIV-infected patients. Continued effort is required in the development and validation of additional POC tests to support HIV patient care, and in quality assurance in manufacturing and in test performance in the field to ensure appropriate use of existing and new POC tests.

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“…The associated image analysis software automatically provides both absolute CD4 counts and the CD4/CD8% ratios from these images [25,26]. A recent study showed similar performance to flow cytometry in an HIV infected cohort [27]. Furthermore, a separate study showed that T cells can be distinguished from monocytes based on their different intensity after staining with fluorescent anti-hCD4 [28].…”

Section: The Need For Point Of Care Technologiesmentioning

confidence: 99%

Emerging technologies for point-of-care CD4 T-lymphocyte counting

Boyle¹,

Hawkins²,

Steele³

et al. 2012

Trends in Biotechnology

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A CD4 T-lymphocyte count determines eligibility for antiretroviral therapy (ART) with patients recently diagnosed with HIV and also monitors the efficacy of ART treatment thereafter. ART slows the progression of HIV to AIDS. In the developing world, CD4 tests are often performed in centralized laboratories, typically in urban areas. The expansion of ART programs into rural areas has created a need for rapid CD4 counting as logistical barriers can delay the timely dissemination of test results and affect patient care through delay in intervention or loss of follow-up care. CD4 measurement at the point-of-care (POC) in rural areas could help facilitating ART and monitoring of treatment. This review highlights recent technology developments with applications towards determining CD4 counts at the POC.

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Clinical evaluation of a simple image cytometer for CD4 enumeration on HIV‐infected patients

Cited by 14 publications

References 20 publications

On-chip sample preparation by controlled release of antibodies for simple CD4 counting

On-chip sample preparation by controlled release of antibodies for simple CD4 counting

Point-of-Care Testing

Emerging technologies for point-of-care CD4 T-lymphocyte counting

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