Clinical Evaluation of the ASTY Colorimetric Microdilution Panel for Antifungal Susceptibility Testing

Pfaller, Michael A.; Arıkan, Sevtap; Lozano-Chiu, Mario; Chen, Y.-S.; Coffman, Stacy L.; Messer, S. A.; Rennie, Robert; Sand, Crystal; Heffner, T.; Rex, John H.; Wang, J.; Yamane, Nobuhisa

doi:10.1128/jcm.36.9.2609-2612.1998

Cited by 37 publications

(17 citation statements)

References 18 publications

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“…(C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, C. krusei, C. lusitaniae, C. guilliermondii, C. lipolytica, C. rugosa and C. zeylanoides) against amphotericin B, 5-fluorocytosine, fluconazole and itraconazole. 62 The ASTY colorimetric microdilution panel method appears to be comparable with the CLSI method for testing the susceptibility of Candida spp. to a variety of antifungal agents.…”

Section: Disk-based Assaysmentioning

confidence: 99%

In vitro susceptibility testing in fungi: a global perspective on a variety of methods

Lass‐Flörl

Perkhofer

Mayr

2010

Mycoses

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Candida and Aspergillus species are the most common causes of invasive fungal infections in immunocompromised patients. The introduction of new antifungal agents and recent reports of resistance emerging during treatment have highlighted the need for in vitro susceptibility testing. For some drugs, there is a supporting in vitro-in vivo correlation available from studies of clinical efficacy. Both intrinsic and emergent antifungal drug resistance are encountered. Various testing procedures have been proposed, including macrodilution and microdilution, agar diffusion, disk diffusion and Etest. Early recognition of infections caused by pathogens that are resistant to one or more antifungals is highly warranted to optimise treatment and patient outcome.

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Section: Disk-based Assaysmentioning

confidence: 99%

In vitro susceptibility testing in fungi: a global perspective on a variety of methods

Lass‐Flörl

Perkhofer

Mayr

2010

Mycoses

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“…The results suggest that the Sensititre and ASTY colorimetric broth microdilution methods are suitable alternatives for routine antifungal susceptibility testing of Candida spp. and C. neoformans, showing excellent agreement (Ն80%) with the NCCLS reference method for amphotericin B, flucytosine, fluconazole, and itraconazole (3,12,20). The FUNG-ITEST breakpoint testing system is a simplified method in which each drug (amphotericin B, flucytosine, fluconazole, ketoconazole, and itraconazole) is tested at only two concentrations to distinguish resistant isolates from susceptible ones.…”

Section: Discussionmentioning

confidence: 93%

“…recovered during prolonged treatment with this agent correlate with therapeutic failure (8,11). Previous evaluations of commercially available antifungal drug susceptibility test methods have included colorimetric broth microdilution methods (Sensititre, Westlake, Ohio, and ASTY, Kyokuto Pharmaceutical Industrial Co., Ltd.) (3,12,20), a noncolorimetric broth breakpoint testing system (FUN-GITEST; Sanofi Diagnostics Pasteur, Paris, France) (2,21), and an agar diffusion system (E-test; AB Biodisk, Solna, Sweden) (4,23). The results suggest that the Sensititre and ASTY colorimetric broth microdilution methods are suitable alternatives for routine antifungal susceptibility testing of Candida spp.…”

Section: Discussionmentioning

confidence: 99%

“…To address the need for simpler and more efficient testing methods, various commercial methods have been developed and evaluated including colorimetric broth microdilution methods (3,12,20), breakpoint testing methods (2,21), and agar diffusion methods (4,23). Likewise, the PASCO Division of Becton-Dickinson has developed a commercially available broth microdilution panel for the in vitro susceptibility testing of antibacterial agents which has been evaluated on a number of occasions (10,19,22).…”

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Comparative Evaluation of PASCO and National Committee for Clinical Laboratory Standards M27-A Broth Microdilution Methods for Antifungal Drug Susceptibility Testing of Yeasts

et al. 2000

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The PASCO antifungal susceptibility test system, developed in collaboration with a commercial company, is a broth microdilution assay which is faster and easier to use than the reference broth microdilution test performed according to the National Committee for Clinical Laboratory Standards (NCCLS) document M27-A guidelines. Advantages of the PASCO system include the system's inclusion of quality-controlled, premade antifungal panels containing 10, twofold serial dilutions of drugs and a one-step inoculation system whereby all wells are simultaneously inoculated in a single step. For the prototype panel, we chose eight antifungal agents for in vitro testing (amphotericin B, flucytosine, fluconazole, ketoconazole, itraconazole, clotrimazole, miconazole, and terconazole) and compared the results with those of the NCCLS method for testing 74 yeast isolates (14 Candida albicans, 10 Candida glabrata, 10 Candida tropicalis, 10 Candida krusei, 10 Candida dubliniensis, 10 Candida parapsilosis, and 10 Cryptococcus neoformans isolates). The overall agreements between the methods were 91% for fluconazole, 89% for amphotericin B and ketoconazole, 85% for itraconazole, 80% for flucytosine, 77% for terconazole, 66% for miconazole, and 53% for clotrimazole. In contrast to the M27-A reference method, the PASCO method classified as resistant seven itraconazole-susceptible isolates (9%), two fluconazole-susceptible isolates (3%), and three flucytosine-susceptible isolates (4%), representing 12 major errors. In addition, it classified two fluconazole-resistant isolates (3%) and one flucytosine-resistant isolate (1%) as susceptible, representing three very major errors. Overall, the agreement between the methods was greater than or equal to 80% for four of the seven species tested (C. dubliniensis, C. glabrata, C. krusei, and C. neoformans). The lowest agreement between methods was observed for miconazole and clotrimazole and for C. krusei isolates tested against terconazole. When the data for miconazole and clotrimazole were removed from the analysis, agreement was >80% for all seven species tested. Therefore, the PASCO method is a suitable alternative procedure for the testing of the antifungal susceptibilities of the medically important Candida spp. and C. neoformans against a range of antifungal agents with the exceptions only of miconazole and clotrimazole and of terconazole against C. krusei isolates.

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“…(Carrillo-Munoz et al, 2006) ASTY colorimetric microdilution panel is another method, which is under investigation. (Pfaller et al, 1998) . Further, there is another non-commercial method which is based on mitochondrial dehydrogenase enzymes reducing tetrazolium salts 2,3-bis {2-methoxy-4-nitro-5-[(sulfonyl amino) carbonyl]-2H-tetrazolium-hydroxide} (XTT).…”

Section: E Testmentioning

confidence: 99%

Antifungal susceptibility testing of candida – Methods and issues in current practice

Krishnasamy¹,

Santharam²,

Saikumar³

2019

ijrps

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Despite the availability of many antifungal drugs in clinical practice, the occurrence of antifungal drug resistance is on the rise. Since the antifungal susceptibility testing (AFST) is not done routinely in many of the microbiology laboratories, it is very difficult to determine which antifungal agent is very effective for a particular infection. There is a real need for precise, reproducible and extrapolative antifungal susceptibility testing methods to aid the therapeutic management. The practice of empirical treatment for fungal infections further promotes the emergence of resistant strains. The AFST practice would essentially help the clinicians in appropriate decision making. Although conventional AFST methods are somewhat cumbersome, many novel AFST methods are currently available in many laboratory settings which would provide a quicker result many times. In essence, the application of AFST along with identification of the fungus up to species level would definitely be very helpful in selecting the primary antifungal agents for treatment especially in difficult to manage and invasive fungal infections. This review will throw light on the various AFST methods available and their issues in the current practice.

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Clinical Evaluation of the ASTY Colorimetric Microdilution Panel for Antifungal Susceptibility Testing

Cited by 37 publications

References 18 publications

In vitro susceptibility testing in fungi: a global perspective on a variety of methods

In vitro susceptibility testing in fungi: a global perspective on a variety of methods

Comparative Evaluation of PASCO and National Committee for Clinical Laboratory Standards M27-A Broth Microdilution Methods for Antifungal Drug Susceptibility Testing of Yeasts

Antifungal susceptibility testing of candida – Methods and issues in current practice

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