Clinical evaluation of the efficiency of an oocyte donation program using egg cryo-banking

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

“…In comparison, a survival rate of 90% [5,8,9,45] has been achieved with vitrification in 2.7 M ethylene glycol + 2.1 M dimethyl sulphoxide + 0.5 M sucrose in an open system initially reported by Katayama et al, (2003) [46]. This level of survival has repeatedly been obtained [10,[47][48][49] but not by all groups (77%; [50], 81%; [51] and when used in conjunction with a closed system has resulted in a reduced survival (75%; [52]). Manipulation of the equilibration of the permeable cryoprotectant and water prior to vitrification in a closed system has resulted in a high rate of survival (95%) only previously observed in open systems but blastocyst development appeared to be reduced [40], possibly as a consequence of ultrastructural damage [53].…”

Live birth with vitrified-warmed oocytes of a chronic myeloid leukemia patient nine years after allogenic bone marrow transplantation

“…Therefore, oocyte cryopreservation is the first option for patients with chronic myeloid leukemia who have no current partners and wish to use a future partner's sperm. Low effectiveness of oocyte cryopreservation is a major limitation, but studies have reported improved pregnancy outcomes [12][13][14][15][16]. Noyes et al [15,16] showed that delivery rate using vitrified oocytes is comparable to that of conventional in vitro fertilization using fresh oocytes, with an ongoing/delivered pregnancy rate of 57%.…”

Effect of vitrification procedure on chromosomal status of embryos achieved from vitrified and fresh oocytes

“…Since the first pregnancy achieved with a vitrified/warmed human oocyte [83] and the first birth of a healthy baby [84], vitrification results have improved significantly during the last decade [17][18][19][20][21][22][23][24][25][26][85][86][87][88]. Vitrification is the glass-like solidification of a solution at low temperature, without ice crystal formation.…”

“…As an alternative to slow freezing, the vitrification procedure has been recently applied for human oocytes and embryos [15,16] allowing to improve the percentages of oocyte survival, fertilization, embryo development rates and clinical outcomes [17][18][19][20][21][22][23][24][25][26]. Vitrification is a non-equilibrium method and may be regarded as a radical approach in which ice crystal formation is totally eliminated.…”