Clinical evaluation of the new Roche platform of serological and molecular cytomegalovirus-specific assays in the diagnosis and prognosis of congenital cytomegalovirus infection

Guideline No. 420: Cytomegalovirus Infection in Pregnancy

“…62 Viral load in amniotic fluid has been investigated as a potential marker for predicting neonatal outcomes, with conflicting results. 50,63,64 Some studies have found an association between higher viral loads and the severity of the disease, while others have not. Ultimately, studies have not consistently shown that low levels or even negative amniotic fluid PCR results rule out neurologic impairment or SNHL.…”

A Randomized Dose-Escalating Phase I Trial of a Replication-Deficient Lymphocytic Choriomeningitis Virus Vector-Based Vaccine Against Human Cytomegalovirus

“…One subject in the medium dose group showed a strong increase in cytomegalovirus-specific responses between Month 4 and 6. Cytomegalovirus infection was confirmed by additional testing with immunoglobulin (Ig)M and IgG immunoblots [ 26 , 27 ] and cytomegalovirus-specific quantitative real-time polymerase chain reaction using cytomegalovirus ELITe MGB kit (ELITech Group) by Tiziana Lazzarotto (University of Bologna, Italy). No symptoms were reported by the subject during this period.…”

Advocating for cytomegalovirus maternal serologic screening in the first trimester of pregnancy: if you do not know where you are going, you will wind up somewhere else

“…[7][8][9]16 Albeit some remaining pitfalls such as the interpretation of virological profile in serum with a low level of IgG, the tools available for the diagnosis of maternal primary infection are reliable. 10,11 The evaluation of the 4 most used CMV IgM assays in Europe reported that the mean sensitivity to diagnose a primary infection in the first trimester of pregnancy was 93.5% (range, 79.4%−98.0%). The strength of the bond (avidity) between the antigen and the IgG antibody increases gradually during the 6 months after primary infection.…”