Clinical evaluation of the QuickVet®/RapidVet® canine dog erythrocyte antigen 1.1 blood-typing test

Kohn, Barbara; Classe, Gabriele; Weingart, Christiane

doi:10.1177/1040638712442880

Cited by 11 publications

(8 citation statements)

References 15 publications

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“…The Quick TEST DEA 1 Alvedia and the RapidVet-H Canine DEA 1.1 are easy to use and were performed by veterinary practitioners. Nevertheless, the proper use and interpretation of the rapid typing test RapidVet-H Canine DEA 1.1, which was largely used in this study, were completed upon request by the Agrolabo, TO, Italy technical support group [8, 21, 22, 42]. …”

Section: Discussionmentioning

confidence: 99%

Prevalence of Dog Erythrocyte Antigen 1 in 7,414 Dogs in Italy

Valentin

Gavazza

Lubas

2017

Veterinary Medicine International

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The study aim was to establish the prevalence of DEA 1, the most immunogenic and clinically important blood group in canine blood transfusion, in 7,414 dogs from Italy. The potential sensitization risk following a first transfusion and the acute reaction risk following a second transfusion given without a cross-matching and blood typing test were also calculated. Dogs tested were purebred (4,798) and mongrel (2,616); 38.8% were DEA 1 negative and 61.2% were DEA 1 positive. High prevalence for DEA 1 positive blood type was found in Ariegeois and English Setter, whereas German Shepherd and Boxer had higher DEA 1 negative blood type. Breeds with blood type never reported before included French Brittany Spaniel and Pug showing a high prevalence of DEA 1 positive type, while French Bulldog and West Highland White Terrier were more often DEA 1 negative. Just 48.8% of purebred and 13.9% of mongrel dogs were considered as prospective blood donors based upon their blood type. Most of the breeds had a sensitization risk of 20.0–25.0%. Rottweiler and Ariegeois had less risk of sensitization (9.4 and 4.2%) and the minor risk of an acute transfusional reaction (0.9–0.2%). The prevalence of DEA 1 positive and negative dogs in Italy agrees with most of the data already reported in the literature.

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Section: Discussionmentioning

confidence: 99%

Prevalence of Dog Erythrocyte Antigen 1 in 7,414 Dogs in Italy

Valentin

Gavazza

Lubas

2017

Veterinary Medicine International

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“…Blood typing methods have evolved from tube and card tests to immunochromatographic strip, cartridge, and flow cytometry assays, but additional typing kits for other blood types would be desirable (1,(21)(22)(23)(24). Several limited surveys have been performed for DEA 1, and a few have assessed other blood types in North America and Europe (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35), but there have been no comprehensive surveys carried out on a canine population typing for many blood groups.…”

Section: Introductionmentioning

confidence: 99%

Survey of Blood Groups DEA 1, DEA 4, DEA 5, Dal, and Kai 1/Kai 2 in Different Canine Breeds From a Diagnostic Laboratory in Germany

et al. 2020

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More than twelve blood group systems have been described in dogs, but little is known about their distribution frequencies within breed populations. Here, we report on an extensive typing survey carried out using available reagents and either established or new clinical kits in purebred dogs from Germany. Leftover anticoagulated blood samples were examined using an immunochromatographic strip method for DEA 1, a gel column technique for Dal and Kai 1/2, and new card agglutination tests for DEA 4 and DEA 5 (which were partially compared with the gel column technique). Monoclonal antibodies were used for DEA 1 and Kai 1/2 typing, and polyclonal antibodies were used for all other types. Among the 206 dogs, 59.2% were DEA 1+, 100% DEA 4+, 9% (Card)/11% (Gel) DEA 5+, 89.3% Dal+, 96.6% Kai 1+, and 2.9% Kai 2+. None of the dogs were Kai 1+/2+, and only one was Kai 1-/2-. Dal-dogs were found in several breeds. Erythrocytes from most DEA 1+ dogs bound strongly on the strips. The agglutination reactions for DEA 5 on the new card tests were generally less than those on the gel column. The blood group pattern DEA 4+, DEA 5-, Dal+, Kai 1+/2-and either DEA 1+ or DEA 1-was found among 80% of the dogs. In this first extensive blood typing survey of purebred dogs from Europe, the proportions of positive and negative blood types were similar to those found in the United States and, for DEA 1, were also similar to those from other European countries, with considerable breed variation in blood types. The newer typing techniques seem to work well and will likely be useful for detecting and preventing specific blood type incompatibilities in the clinic.

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“…5,6,10,13 Two murine monoclonal anti-DEA 1.1 antibodies, introduced in the 1990s, are used in typing cards, b gel columns, c and immunochromatographic strips. d, 13,[17][18][19][20][21] It has been suspected that DEA 1.2+ blood gives a weakly positive DEA 1.1 result. 13 Because these monoclonal antibodies were never properly evaluated against dogs which tested DEA 1.1+ or DEA 1.2+, we will refer to them as anti-DEA 1 rather than anti-DEA 1.1 antibodies.…”

mentioning

confidence: 99%

DEA1 Expression on Dog Erythrocytes Analyzed by Immunochromatographic and Flow Cytometric Techniques

Acierno

Raj

Giger

2014

Veterinary Internal Medicne

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BackgroundThe Dog erythrocyte antigen (DEA) 1 blood group system was thought to contain types DEA 1.1 and 1.2 (and possibly 1.3 [A3]). However, DEA 1.2+ dogs are very rare and newer typing methods reveal varying degrees of DEA 1 positivity.ObjectivesTo assess if variation in DEA 1 positivity is because of quantitative differences in surface antigen expression. To determine expression patterns in dogs over time and effects of blood storage (4°C). To evaluate DEA 1.2+ samples by DEA 1 typing methods.AnimalsAnticoagulated blood samples from 66 dogs in a research colony and from a hospital, and 9 previously typed DEA 1.2+ dogs from an animal blood bank.MethodsResearch study: Samples were analyzed by flow cytometry and immunochromatographic strip using a monoclonal anti‐DEA 1 antibody.ResultsTwenty dogs were DEA 1−, whereas 46 dogs were weakly to strongly DEA 1+. Antigen quantification revealed excellent correlation between strip and flow cytometry (r = 0.929). Both methods reclassified DEA 1.2+ samples as weakly to moderately DEA 1+, but they were not retyped with the polyclonal anti‐DEA 1.1/1.X antibodies. Dogs and blood samples retained their relative DEA 1 antigen densities over time.Conclusions and Clinical ImportanceThe blood group system DEA 1 is a continuum from negative to strongly positive antigen expression. Previously typed DEA 1.2+ appears to be DEA 1+. These findings further the understanding of the DEA 1 system and suggest that all alleles within the DEA 1 system have a similarly based epitope recognized by the monoclonal antibody.

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Clinical evaluation of the QuickVet®/RapidVet® canine dog erythrocyte antigen 1.1 blood-typing test

Cited by 11 publications

References 15 publications

Prevalence of Dog Erythrocyte Antigen 1 in 7,414 Dogs in Italy

Prevalence of Dog Erythrocyte Antigen 1 in 7,414 Dogs in Italy

Survey of Blood Groups DEA 1, DEA 4, DEA 5, Dal, and Kai 1/Kai 2 in Different Canine Breeds From a Diagnostic Laboratory in Germany

DEA1 Expression on Dog Erythrocytes Analyzed by Immunochromatographic and Flow Cytometric Techniques

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