Clinical evaluation of the renin-aldosterone system: Comparison of two methods in different clinical conditions

Horváth, Dorottya; Lőcsei, Zoltán; Csizmadia, Zsuzsanna; Toldy, Erzsébet; Szabolcs, I.; Rácz, Kàroly

doi:10.1556/oh.2012.29476

Cited by 1 publication

(1 citation statement)

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“…These are mainly automated non-isotopic determinations of aldosterone and renin, from which we tested CLIA. It seems that for the diagnostics of PH are preferred non-isotopic direct determinations of both aldosterone and renin and their ratio value as well (Dorrian et al, 2010;Horváth et al, 2012;Burrello et al, 2015;Glinicki et al, 2015). PH laboratory diagnostics is based over last 45 years also on the ratio of aldosterone and renin or renin activity respectively (Ferrari et al, 2004).…”

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confidence: 99%

Comparison of the Chemiluminescence Immunoassay LIAISON® with the Radioimmunoassay for Aldosterone and Renin Measurement

et al. 2021

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Determination of renin plasma levels is useful in the diagnosis of hypertension and in the therapeutic follow-up of hypertensive patients. Plasmatic concentration of renin decreases in patients with hypertension due to a primary hyperaldosteronism, contrary to renovascular hypertension where concentrations of renin and aldosterone are both elevated. Blood samples (serum, EDTA plasma) were analysed using two different chemiluminiscent methods CLIA LIAISON® and radioimmunoassay for aldosterone (IMMUNOTECH Beckman Coulter) and renin (Cisbio Bioassay) measurements were compared. We used both methods to ascertain the correlation between serum vs. EDTA plasma levels of aldosterone (RIA, CLIA) and renin (IRMA, CLIA) and to compare aldosterone to renin ratios for CLIA and for radioimmunoassay: serum aldosterone to plasma renin and plasma aldosterone to plasma renin. We compared serum aldosterone CLIA vs. RIA (rP=0.933, P<0.001) and plasma renin determined using CLIA vs. IRMA (rP=0.965, P=0.062). Furthermore, we used both methods to establish the correlation between the serum vs. plasma levels of aldosterone: RIA (rP=0.980, P<0.001); CLIA (rP=0.994, P=0.353) and serum vs. plasma levels of renin: IRMA (rP=0.948, P<0.001); CLIA (rP=0.921, P=0.011). Aldosterone (serum, plasma) to plasmatic renin ratios for CLIA (rP=0.999, P=0.286) and for radioimmunoassay (rP=0.992, P=0.025). Our data demonstrate that renin and aldosterone concentrations obtained using CLIA correlate with renin and aldosterone concentrations using radioimmunoassay methods. Correlation coefficients of pair results ranged from 0.921 to 0.994. Aldosterone (serum, EDTA plasma) to plasmatic renin ratios are comparable and any of them can be used with no significant differences found.

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