Clinical factors influencing the performance of bacterial multiplex polymerase chain reaction in patients with community-onset pneumonia

Park, Ga Eun; Peck, Kyong Ran; Ko, Jae-Hoon; Kang, Cheol-In; Cho, Sun Young; Chung, Doo Ryeon; Lee, Nam Yong

doi:10.1007/s10096-019-03741-6

Cited by 3 publications

(4 citation statements)

References 23 publications

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“…Among pneumonia cases, eight patients had bacteremia (7.3%) while 298 patients did not (46%). It may be that, with pneumonia, it is only possible to diagnose the causative pathogen in 30-40% of cases using conventional diagnostic methods (40). ROC curve analysis demonstrated that PCT was superior to presepsin and CRP as a diagnostic biomarker, and it had higher sensitivity and negative predictive value for predicting culture-proven bacterial infection and bacteremia.…”

Section: Discussionmentioning

confidence: 99%

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Performance of presepsin and procalcitonin predicting culture-proven bacterial infection and 28-day mortality: A cross sectional study

Park

Yoon

et al. 2022

Front. Med.

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Presepsin is a highly specific biomarker for diagnosing bacterial infections, but its clinical usefulness is not well validated. A retrospective cross-sectional study was conducted. Among the patients suspected bacterial infection or fulfilled the criteria of systemic inflammatory response syndrome (SIRS) and patients who underwent blood culture, presepsin, procalcitonin (PCT), and C-reactive protein (CRP) at the same time were included. Receiver operating characteristic (ROC) curve analysis and logistic regression were used to compare performance of three biomarkers. A total of 757 patients were enrolled, including 256 patients (33.8%) with culture-proven bacterial infection and 109 patients (14.4%) with bacteremia. The 28-day mortality rate was 8.6%. ROC curve analysis revealed that the area under the curve (AUC) of PCT was higher than that of presepsin for both culture-proven bacterial infection (0.665 and 0.596, respectively; p = 0.003) and bacteremia (0.791 and 0.685; p < 0.001). In contrast, AUC of PCT for 28-day mortality was slower than presepsin (0.593 and 0.720; p = 0.002). In multivariable logistic regression analysis, PCT showed the highest ORs for culture-proven bacterial infection (OR 2.23, 95% CI 1.55–3.19; p < 0.001) and for bacteremia (OR 5.18, 95% CI 3.13–8.56; p < 0.001), while presepsin showed the highest OR for 28-day mortality (OR 3.31, 95% CI 1.67–6.54; p < 0.001). CRP did not show better performance than PCT or presepsin in any of the analyses. PCT showed the best performance predicting culture-proven bacterial infection and bacteremia, while presepsin would rather be useful as a prognostic marker.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Performance of presepsin and procalcitonin predicting culture-proven bacterial infection and 28-day mortality: A cross sectional study

Park

Yoon

et al. 2022

Front. Med.

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“…Multiplex PCR has been widely used in a variety of fields of genetic testing and showed higher detection efficiency and lower cost compared to conventional PCR 15–17 . However, at least two shortcomings remain.…”

Section: Introductionmentioning

confidence: 99%

“…[10][11][12][13][14] Multiplex PCR has been widely used in a variety of fields of genetic testing and showed higher detection efficiency and lower cost compared to conventional PCR. [15][16][17] However, at least two shortcomings remain. The first is poor consistency of amplification efficiency between different targets, 18 and the second is the false-positive/false-negative caused by primer-primer interactions and primer-amplicon interactions.…”

Section: Introductionmentioning

confidence: 99%

Developing a multiplex PCR‐based assay kit for bloodstream infection by analyzing genomic big data

Zhang

Luo²,

Zeng³

et al. 2022

Clinical Laboratory Analysis

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Background In recent years, the incidence of bloodstream infections (BSI) has increased, the composition of pathogenic bacteria has changed, and drug resistance among bacteria has gradually increased due to the widespread use of interventional techniques, broad‐spectrum antibacterial drugs, hormones, and immunosuppressive agents. Here, we have developed a multiplex PCR assay kit for the detection of pathogens (14 Gram‐negative bacteria, 15 Gram‐positive bacteria, and 4 fungi) in whole blood from patients with BSI using five‐color fluorescent multiplex PCR followed by capillary electrophoresis. Our assay exhibits a diagnosis of higher quality and an improved detection rate for common pathogens. Methods A local genome DNA database of 33 pathogenic bacteria was constructed. Next, “Exhaustive” primer search of the full coding sequence of the reference genomes of these bacteria was performed. Panels with minimal interactions between primers and amplicons were selected by random sampling and testing by a recursive algorithm. Primers and Mg2+ concentrations and PCR reaction procedures were optimized to maximize the detection efficacy. Results The LOD of the kit was determined as 100 copies/μl. Using clinical samples, results generated by this kit and regular blood culture method were found to be 95.08% consistent. Additionally, six pathogens which were unidentifiable by blood culture were successfully detected by this kit. Conclusion Our study provided a bioinformatics approach to the challenge of primer design in multiplex PCR, and combined with optimized wet lab practice, a multiplex PCR‐based assay kit for BSI with higher sensitivity and accuracy than blood culture was produced.

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Disease burden and macrolide resistance of Mycoplasma pneumoniae infection in adults in the Asia-Pacific region

Chih-Cheng Lai,

Hsueh,

Hsu

et al. 2024

International Journal of Antimicrobial Agents

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Clinical factors influencing the performance of bacterial multiplex polymerase chain reaction in patients with community-onset pneumonia

Cited by 3 publications

References 23 publications

Performance of presepsin and procalcitonin predicting culture-proven bacterial infection and 28-day mortality: A cross sectional study

Performance of presepsin and procalcitonin predicting culture-proven bacterial infection and 28-day mortality: A cross sectional study

Developing a multiplex PCR‐based assay kit for bloodstream infection by analyzing genomic big data

Disease burden and macrolide resistance of Mycoplasma pneumoniae infection in adults in the Asia-Pacific region

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