Clinical feasibility of two-step streptavidin/111In-biotin scintigraphy in patients with suspected vertebral osteomyelitis

Lazzeri, Elena; Pauwels, E. K. J.; Erba, Paola Anna; Volterrani, Duccio; Manca, Mario; Bodei, Lisa; Trippi, D; Bottoni, Antonio; Cristofani, Renza; Consoli, V.; Palestro, Christopher J.; Mariani, Giuliano

doi:10.1007/s00259-004-1581-2

Cited by 47 publications

(20 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Approximately 50% of cases present as areas of nonspecific decreased activity (4). (19,20). 18 F-FDG imaging consistently outperforms bone, 67 Ga, and antigranulocyte antibody imaging and compares favorably with MRI (21-30).…”

Section: Spondylodiskitismentioning

confidence: 91%

Radionuclide Imaging of Musculoskeletal Infection: A Review

Palestro

2016

J Nucl Med

Self Cite

View full text Add to dashboard Cite

Learning Objectives: On successful completion of this activity, participants should be able to describe (1) the principle of combined labeled leukocyte/marrow imaging; (2) at least one advantage of 18 F-FDG PET or PET/CT over MRI for diagnosing spondylodiskitis; and (3) at least one advantage of SPECT/CT over planar imaging in the diagnosis of pedal osteomyelitis in diabetic patients.Financial Disclosure: The author of this article has indicated no relevant relationships that could be perceived as a real or apparent conflict of interest. CME Credit: SNMMI is accredited by the Accreditation Council for Continuing Medical Education (ACCME) to sponsor continuing education for physicians. SNMMI designates each JNM continuing education article for a maximum of 2.0 AMA PRA Category 1 Credits. Physicians should claim only credit commensurate with the extent of their participation in the activity. For CE credit, SAM, and other credit types, participants can access this activity through the SNMMI website (http://www.snmmilearningcenter.org) through September 2019.There are numerous imaging tests for diagnosing musculoskeletal infection. Radiographs are routinely performed, because even when not diagnostic, they provide an anatomic overview of the region of interest that could influence subsequent procedure selection and interpretation. MRI is sensitive and provides superb anatomic detail. Bone scintigraphy accurately diagnoses osteomyelitis in bones not affected by underlying conditions. 67 Ga is used primarily for spondylodiskitis. Although in vitro labeled leukocyte imaging is the radionuclide test of choice for complicating osteomyelitis such as diabetic pedal osteomyelitis and prosthetic joint infection, it is not useful for spondylodiskitis. Antigranulocyte antibodies and antibody fragments have limitations and are not widely available. 111 In-biotin is useful for spondylodiskitis. Radiolabeled synthetic fragments of the antimicrobial peptide ubiquicidin are promising infection-specific agents. 18 F-FDG is the radiopharmaceutical of choice for spondylodiskitis. Its role in diabetic pedal osteomyelitis and prosthetic joint infection is not established. Preliminary data suggest 68 Ga may be useful in musculoskeletal infection. 124 I-fialuridine initially showed promise as an infection-specific radiopharmaceutical, but subsequent investigations were disappointing. The development of PET/CT and SPECT/CT imaging systems, which combine anatomic and functional imaging, has revolutionized diagnostic imaging. These hybrid systems are redefining the diagnostic workup of patients with suspected or known infection and inflammation by improving diagnostic accuracy and influencing patient management.

show abstract

Section: Spondylodiskitismentioning

confidence: 91%

Radionuclide Imaging of Musculoskeletal Infection: A Review

Palestro

2016

J Nucl Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Radiolabeled biotin derivatives can be used as targeted diagnostic and therapeutic radiopharmaceuticals in nuclear medicine. They have been evaluated in the clinical practice for the scintigraphic diagnosis of skeletal infections with favorable results . In addition, radiolabeled biotin derivatives have been evaluated in cancer pretargeted radioimmunoscintigraphy (RIS) and radioimmunotherapy (RIT), an approach with considerable advantages over the conventional RIS/RIT.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and biological evaluation of new biotinylated ^99mTc/Re‐tricarbonyl complexes

Makris

Papagiannopoulou

2016

Labelled Comp Radiopharmac

View full text Add to dashboard Cite

The synthesis and biological evaluation of three new biotinylated fac-[(99m)Tc/Re(CO)3](+) complexes with the tridentate ligands L1, L2, and L3 are reported. L1-L3 contain the chelators 2-((5-aminopentyl)(pyridin-2-ylmethyl)amino)acetic acid, 2-(2-aminoethylthio)-3-(1H-imidazol-4-yl)propanoic acid, and 2-amino-3-(1-carboxy-2-(1H-imidazol-4-yl)ethylthio)propanoic acid, respectively, which are conjugated to biotin's carboxylate via their amine group. The fac-[Re(CO)3(L1-L3)] complexes were synthesized and characterized by NMR and IR, where the (N,N,O) coordination for ReL1 and the (N,S,O) coordination for ReL2 and ReL3 were confirmed. The tracer complexes fac-[(99m)Tc(CO)3(L1-L3)] were synthesized in high yield and were found highly stable in 10(-3) M L-histidine and L-cysteine over 24 h. Furthermore, they exhibited high binding affinity (>90%) for avidin. Rat plasma studies showed complete cleavage of biotin from (99m)TcL1 after 1 h and a low percentage of intact (99m)TcL2 and (99m)TcL3 with no biotin cleavage metabolites present, over 24 h. Similarly, rat urine analysis showed the presence of intact (99m)TcL2 and (99m)TcL3, while (99m)TcL1 was cleaved. Biodistribution studies of (99m)TcL2 and (99m)TcL3 revealed fast blood and tissue clearance.

show abstract

“…Its high affinity for biotin has been used for a variety of biological and analytical research by coupling with various reporter probes, such as fluorescence dyes, radioactive elements or enzymes. In addition, biotin-streptavidin system is also applied to human clinical medicine for cancer diagnosis, radioactive imaging and pre-targeted immunotherapy [3,4,5,6]. Previous studies reported that administration of streptavidin after heart transplant prolonged survival and streptavidin suppressed T cell proliferation in mixed splenocyte culture [7,8].…”

Section: Introductionmentioning

confidence: 99%

Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression

et al. 2012

View full text Add to dashboard Cite

Streptavidin is widely used as a detection tool in biology research because of its high affinity and specificity binding to biotin. Biotin-streptavidin system has also been explored for detection of infection and tumor in clinical medicine. Here, we show immunosuppressive property of streptavidin on T cell activation and proliferation. Upon CD3 and CD28 stimulation, CD4+ T cells produce interleukin 2 (IL-2) and express IL-2 receptor α chain (CD25). Addition of streptavidin in T cell culture suppressed IL-2 synthesis and CD25 expression with no cytotoxicity. The immunosuppressive effect of streptavidin was reversed by excessive biotin. Conjugated to a single chain anti-CD7 variable fragment (scFvCD7), streptavidin was directly delivered to T cells and showed substantially more profound suppressive effect on T cell activation. These results suggest that streptavidin could potentially be used as a novel immunomodulator.

show abstract

Clinical feasibility of two-step streptavidin/111In-biotin scintigraphy in patients with suspected vertebral osteomyelitis

Abstract: Streptavidin/111In-biotin scintigraphy is highly sensitive and specific for detecting vertebral osteomyelitis in the first 2 weeks after the onset of clinical symptoms, and is potentially very useful for guiding clinical decisions on instituting appropriate therapy.

Cited by 47 publications

References 34 publications

Radionuclide Imaging of Musculoskeletal Infection: A Review

Radionuclide Imaging of Musculoskeletal Infection: A Review

Synthesis, characterization, and biological evaluation of new biotinylated ^99mTc/Re‐tricarbonyl complexes

Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression

Contact Info

Product

Resources

About

Clinical feasibility of two-step streptavidin/111In-biotin scintigraphy in patients with suspected vertebral osteomyelitis

Abstract: Streptavidin/111In-biotin scintigraphy is highly sensitive and specific for detecting vertebral osteomyelitis in the first 2 weeks after the onset of clinical symptoms, and is potentially very useful for guiding clinical decisions on instituting appropriate therapy.

Cited by 47 publications

References 34 publications

Radionuclide Imaging of Musculoskeletal Infection: A Review

Radionuclide Imaging of Musculoskeletal Infection: A Review

Synthesis, characterization, and biological evaluation of new biotinylated 99mTc/Re‐tricarbonyl complexes

Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression

Contact Info

Product

Resources

About

Synthesis, characterization, and biological evaluation of new biotinylated ^99mTc/Re‐tricarbonyl complexes