Clinical Feature of Intrahepatic B-Lymphocytes in Chronic Hepatitis B

Ashraf, Muhammad; Naderi, Elnaz; Sotoudeh, Masoud; Katoonizadeh, Aezam; Montazeri, Ghodratollah

doi:10.1155/2014/896864

Cited by 15 publications

(9 citation statements)

References 18 publications

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“…The enumeration of CD20+ B-cells was performed using a +40 lens corresponding to a tissue area of 30 mm2 and immunohistochemical scores were assigned by consensus between two pathologists (FD, AB). The slides were visually estimated, and proportions of CD20 positive cells were scored and classified in 5 groups (score 1: 0–10%; score 2: 10–25%; score 3: 25–50%; score 4: 50–75%; score 5: >75% positive cells) as described [ 43 ]. The counts were performed counting the number of CD20+ cells over 100 cells for each area.…”

Section: Methodsmentioning

confidence: 99%

Patients with Tuberculosis Have a Dysfunctional Circulating B-Cell Compartment, Which Normalizes following Successful Treatment

Joosten

Meijgaarden

Nonno³

et al. 2016

PLoS Pathog

140

118

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B-cells not only produce immunoglobulins and present antigens to T-cells, but also additional key roles in the immune system. Current knowledge on the role of B-cells in infections caused by intracellular bacteria is fragmentary and contradictory. We therefore analysed the phenotypical and functional properties of B-cells during infection and disease caused by Mycobacterium tuberculosis (Mtb), the bacillus causing tuberculosis (TB), and included individuals with latent TB infection (LTBI), active TB, individuals treated successfully for TB, and healthy controls. Patients with active or treated TB disease had an increased proportion of antibodies reactive with mycobacteria. Patients with active TB had reduced circulating B-cell frequencies, whereas only minor increases in B-cells were detected in the lungs of individuals deceased from TB. Both active TB patients and individuals with LTBI had increased relative fractions of B-cells with an atypical phenotype. Importantly, these B-cells displayed impaired proliferation, immunoglobulin- and cytokine- production. These defects disappeared upon successful treatment. Moreover, T-cell activity was strongest in individuals successfully treated for TB, compared to active TB patients and LTBI subjects, and was dependent on the presence of functionally competent B-cells as shown by cellular depletion experiments. Thus, our results reveal that general B-cell function is impaired during active TB and LTBI, and that this B-cell dysfunction compromises cellular host immunity during Mtb infection. These new insights may provide novel strategies for correcting Mtb infection-induced immune dysfunction towards restored protective immunity.

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Section: Methodsmentioning

confidence: 99%

Patients with Tuberculosis Have a Dysfunctional Circulating B-Cell Compartment, Which Normalizes following Successful Treatment

Joosten

Meijgaarden

Nonno³

et al. 2016

PLoS Pathog

140

118

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“…In our study, CXCL13 was highly expressed in the ectopic GC of CHB liver, co-localized with KC, Tfh cells, and B cells, indicating that KC-expressed CXCL13 served as a critical regulator for the recruitment of CXCR5 + lymphocytes in CHB patients. Previously, the immunohistochemical findings in liver biopsies suggested a major role for B-cells in the pathogenesis of CHB, which appeared to be driven by increased CD20 + B-cells ( 32 ). In addition, we also completed a comprehensive gene-chip analysis of intrahepatic gene expression in patients at different phases of HBV infection.…”

Section: Discussionmentioning

confidence: 99%

Elevated Expression of Chemokine CXCL13 in Chronic Hepatitis B Patients Links to Immune Control during Antiviral Therapy

et al. 2017

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C–X–C-chemokine ligand 13 (CXCL13), the ligand for C–X–C chemokine receptor type 5 (CXCR5), is a major regulator of B-cell trafficking and plays an integral role in age-dependent clearance of hepatitis B virus (HBV) in the mouse model. However, the expression and function of CXCL13 in patients with chronic hepatitis B (CHB) remain unknown. By use of liver cell subpopulations isolated from CHB patients, we found that CXCL13 mRNA was abundantly expressed in Kupffer cells (KCs), but not in primary hepatocytes, liver sinusoidal endothelial cells, and hepatic stellate cells. Interestingly, KC isolated from HBV-positive liver had much higher level of CXCL13 expression than non-HBV-infected controls. And its expression was induced by toll-like receptor 3 ligand poly I:C stimulation. Moreover, intense expression of CXCL13 protein and accumulation of CD4+ T and B cells were evident in follicular-like structures in the liver tissue of CHB patients, which indicated its chemotactic effect on CXCR5+ CD4+ cells and B cells. Consistently, the levels of serum CXCL13 were significantly higher in the CHB patients than in healthy controls. Furthermore, CXCL13 concentration was increased in the complete response (CR) group during weeks 0–12 and did not change significantly during the course of telbivudine treatment, compared with the patients who didn’t achieve CR. In conclusion, the HBV-related increase of CXCL13 production in KC and serum CXCL13 level during telbivudine treatment might be associated with immune control of chronic HBV infection.

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“…The immune system hires this approach as defense mechanism against pathogenic organism DNA by direct oxidative damage (20). However, the increase of ROS production, as a result of chronic HBV infection, and persistent activation of Kupffer cells can eventually develop the necroinflammatory liver disease and hepatocarcinogenesis (7,8,21).…”

Section: Discussionmentioning

confidence: 99%

8-Hydroxy-2'-Deoxyguanosin in Peripheral Leukocyte Associated With HBsAg in Patients with Chronic Hepatitis B

Ashraf

Moafi

Fazli

et al. 2017

Jundishapur J Microbiol

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Background: Chronic hepatitis B (CHB), with accumulation of Hepatitis B surface Antigen (HBsAg) in hepatocytes, linked to the immune-mediated hepatic inflammation and induction of oxidative stress. 8-Hydroxyl-2'-deoxyguanosine (8-OHdG) is a useful biomarker for measuring the adverse effects of exogenous infectious agents in oxidative damage to DNA. Objectives: The current study aimed at investigating the possible oxidative adverse effects of HBsAg and systemic DNA damage in patients with CHB, and supporting the host-viral interaction in immune-mediated inflammatory. Methods: Thirty patients with CHB who had undergone liver biopsies for therapeutic purposes and 30 matched controls from a healthy population were randomly selected in the present study for assessment of 8-OHdG levels in peripheral blood leukocytes DNA by 32P-postlabeling analysis. Expression of HBsAg in hepatocytes was evaluated immunohistochemically in liver biopsies of patients with CHB. The effect of 8-OHdG and 95% confidence interval (CI), adjusted by relevant confounders, were assessed on hepatitis B virus (HBV) infection.

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Clinical Feature of Intrahepatic B-Lymphocytes in Chronic Hepatitis B

Cited by 15 publications

References 18 publications

Patients with Tuberculosis Have a Dysfunctional Circulating B-Cell Compartment, Which Normalizes following Successful Treatment

Patients with Tuberculosis Have a Dysfunctional Circulating B-Cell Compartment, Which Normalizes following Successful Treatment

Elevated Expression of Chemokine CXCL13 in Chronic Hepatitis B Patients Links to Immune Control during Antiviral Therapy

8-Hydroxy-2'-Deoxyguanosin in Peripheral Leukocyte Associated With HBsAg in Patients with Chronic Hepatitis B

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