Clinical features of Vibrio vulnificus infections in the coastal areas of the Ariake Sea, Japan

Matsumoto, Kouichi; Ohshige, Kenji; Fujita, Naoya; Tomita, Yukiko; Mitsumizo, Shinji; Nakashima, Mitsuyoshi; Oishi, Hirotaka

doi:10.1007/s10156-010-0050-z

Cited by 32 publications

(21 citation statements)

References 11 publications

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“…Therefore, it should be recognized that E tarda can also result in necrotizing fasciitis type 3. In addition, previous reports indicate that the mortality rate of ETB is nearly 50%, which is similar to that of V vulnificus ( 55 ) or severe infections caused by Aeromonas species ( 56 , 57 ).…”

Section: Discussionsupporting

confidence: 60%

Edwardsiella tarda Bacteremia. A Rare but Fatal Water‐ and Foodborne Infection: Review of the Literature and Clinical Cases from a Single Centre

Hirai

Asahata-Tago

Ainoda

et al. 2015

Canadian Journal of Infectious Diseases and Medical Microbiolog

129

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Section: Discussionsupporting

confidence: 60%

Edwardsiella tarda Bacteremia. A Rare but Fatal Water‐ and Foodborne Infection: Review of the Literature and Clinical Cases from a Single Centre

Hirai

Asahata-Tago

Ainoda

et al. 2015

Canadian Journal of Infectious Diseases and Medical Microbiolog

129

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“…People who are most susceptible to V. vulnificus infection usually suffer from a chronic liver disease, primarily cirrhosis or alcoholic liver disease, diabetes mellitus, or diseases associated with iron overload such as hemochromatosis and thalassemia major (Oliver, 2006 ). Worldwide, the vast majority of human disease has been reported from USA and Southeast Asia due to dietary habits of eating raw or undercooked seafood (Tacket et al, 1984 ; Klontz et al, 1988 ; Park et al, 1991 ; Chuang et al, 1992 ; Kumamoto and Vukich, 1998 ; Chiang and Chuang, 2003 ; Matsumoto et al, 2010 ). Reports from other parts of the world have been largely sporadic and typically due to wound infection (Bock et al, 1994 ; Melhus et al, 1995 ; Dalsgaard et al, 1996 ; Horre et al, 1998 ; Torres et al, 2002 ; Frank et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic analysis of clinical and environmental Vibrio vulnificus isolates revealed biotype 3 evolutionary relationships

et al. 2015

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In 1996 a common-source outbreak of severe soft tissue and bloodstream infections erupted among Israeli fish farmers and fish consumers due to changes in fish marketing policies. The causative pathogen was a new strain of Vibrio vulnificus, named biotype 3, which displayed a unique biochemical and genotypic profile. Initial observations suggested that the pathogen erupted as a result of genetic recombination between two distinct populations. We applied a whole genome shotgun sequencing approach using several V. vulnificus strains from Israel in order to study the pan genome of V. vulnificus and determine the phylogenetic relationship of biotype 3 with existing populations. The core genome of V. vulnificus based on 16 draft and complete genomes consisted of 3068 genes, representing between 59 and 78% of the whole genome of 16 strains. The accessory genome varied in size from 781 to 2044 kbp. Phylogenetic analysis based on whole, core, and accessory genomes displayed similar clustering patterns with two main clusters, clinical (C) and environmental (E), all biotype 3 strains formed a distinct group within the E cluster. Annotation of accessory genomic regions found in biotype 3 strains and absent from the core genome yielded 1732 genes, of which the vast majority encoded hypothetical proteins, phage-related proteins, and mobile element proteins. A total of 1916 proteins (including 713 hypothetical proteins) were present in all human pathogenic strains (both biotype 3 and non-biotype 3) and absent from the environmental strains. Clustering analysis of the non-hypothetical proteins revealed 148 protein clusters shared by all human pathogenic strains; these included transcriptional regulators, arylsulfatases, methyl-accepting chemotaxis proteins, acetyltransferases, GGDEF family proteins, transposases, type IV secretory system (T4SS) proteins, and integrases. Our study showed that V. vulnificus biotype 3 evolved from environmental populations and formed a genetically distinct group within the E-cluster. The unique epidemiological circumstances facilitated disease outbreak and brought this genotype to the attention of the scientific community.

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“…This microorganism infects humans by the consumption of contaminated seafood or contact of a wound with seawater and causes necrotizing fasciitis and sepsis. The mortality rate is high (>50%), and the latency period is short (within 24 to 48 h) [1], [2]. Patients with underlying disease such as liver dysfunction, alcoholic cirrhosis, or hemochromatosis are particularly susceptible to this life-threatening infection [1].…”

Section: Introductionmentioning

confidence: 99%

Distribution of Virulence Markers among Vibrio vulnificus Isolates of Clinical and Environmental Origin and Regional Characteristics in Japan

et al. 2013

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Background Vibrio vulnificus is an opportunistic human pathogen that is widely distributed in estuarine environments and is capable of causing necrotizing fasciitis and sepsis. In Japan, based on epidemiological research, the incidences of V. vulnificus were concentrated in Kyusyu, mainly in coastal areas of the Ariake Sea. To examine the virulence potential, various genotyping methods have recently been developed. This study aimed to investigate the distribution of virulence markers among V. vulnificus isolates of clinical and environmental origin in three coastal areas with different infection incidences and to determine whether these isolates have the siderophore encoding gene viuB.Methodology/Principal FindingsWe examined the distribution of genotypes of the 16S ribosomal ribonucleic acid (rRNA) gene, vvhA, vcg, and capsular polysaccharide (CPS), and the presence of viuB in 156 isolates collected from patients and environmental samples in Japan. The environmental samples were collected from three coastal areas: the Ariake Sea, Ise & Mikawa Bay, and Karatsu Bay. The results showed disparity in the ratios of genotypes depending on the sample origins. V. vulnificus isolates obtained from patients were classified into the clinical type for all genotypes. In the environmental isolates, the ratios of the clinical type for genotypes of the 16S rRNA gene, vvhA, and vcg were in the order of the Ariake Sea>Ise & Mikawa Bay>Karatsu Bay. Meanwhile, CPS analysis showed no significant difference. Most isolates possessed viuB.ConclusionsMany V. vulnificus belonging to the clinical type existed in the Ariake Sea. Three coastal areas with different infection incidences showed distinct ratios of genotypes. This may indicate that the distribution of clinical isolates correlates with the incidence of V. vulnificus infection.

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Clinical features of Vibrio vulnificus infections in the coastal areas of the Ariake Sea, Japan

Cited by 32 publications

References 11 publications

Edwardsiella tarda Bacteremia. A Rare but Fatal Water‐ and Foodborne Infection: Review of the Literature and Clinical Cases from a Single Centre

Edwardsiella tarda Bacteremia. A Rare but Fatal Water‐ and Foodborne Infection: Review of the Literature and Clinical Cases from a Single Centre

Comparative genomic analysis of clinical and environmental Vibrio vulnificus isolates revealed biotype 3 evolutionary relationships

Distribution of Virulence Markers among Vibrio vulnificus Isolates of Clinical and Environmental Origin and Regional Characteristics in Japan

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