Fifty-five female buffaloes suffering from uterine torsion were presented to the clinic of Veterinary Gynaecology and Obstetrics, CVAS, Navania, Udaipur, Rajasthan for treatment. Three times blood samples were obtained from 55 buffaloes with uterine torsion (at the time of presentation of the animal, 1 h and 24 h after fetal delivery) and 10 healthy buffaloes to investigate the relationship between concentrations of aspartate amino transferase (AST), alanine aminotransferase (ALT), Bilirubin, serum creatinine and blood urea nitrogen (BUN). The aim of this study was to investigate related alterations in these blood constituents. There were significant (p<0.01) increases in AST, ALT, Bilirubin, serum creatinine and BUN in the affected buffaloes, possibly due to high uterine tissue damage. It may be inferred that torsion may lead to imbalance in biochemical profiles that affect the proper functioning of the uterine musculature. Buffaloes subjected to uterine torsion are associated with hepatic and renal dysfunction. In conclusion, concentration of above parameters can be used as a prognostic indicator for the occurrence of uterine torsion in buffaloes.