Clinical immunology Impact on red blood cell immunity patterns in postoperative phase following total hip arthroplasty

Abstract

ObjectiveIn this study, we aimed to measure changes in red blood cell (RBC) immunity and cytokine levels after performing total hip replacement surgery.Material and methodsTwenty patients receiving total hip arthroplasty were investigated by measuring presurgical and postoperative RBC natural tumor erythrocyte rosette rate (NTERR), RBC C3b receptor rosette rate (RC3bRR), RBC membrane CD35, CD58 and CD59 expression and cytokine levels [including tumor necrosis factor α (TNF-α), interleukin 2 (IL-2), interferon γ (IFN-γ), interleukin 10 (IL-10) and prostaglandin E2 (PGE2)]. Blood samples were collected on the day before surgery and on the first day after hip arthroplasty.ResultsPostoperative NTERR and RC3bRR were significantly lower than presurgical levels (p < 0.05). The RBC membrane CD35, CD58 and CD59 expressions were significantly decreased in the postoperative phase compared to pre-operative levels. Importantly, RBC promoting lymphocyte proliferation rates were significantly reduced after surgery. In addition, postoperative TNF-α, IL-2 and IFN-γ levels in RBC and lymphocyte culture fluid were lower than those pre-operation, whereas IL-10 and PGE2 were significantly increased compared to presurgical levels (p < 0.05).ConclusionsThe modification of RBC immune function may be involved in the occurrence and development of the infection following hip arthroplasty, and this suggests a novel strategy to prevent such infection.

Red blood cells exposed to cancer cells in culture have altered cytokine profiles and immune function

“…These results also demonstrated that RBCs isolated from healthy individuals could be manipulated to alter their cytokine profile and their influence on T-cells. Similar dysregulation of RBC activity has been demonstrated in disease (carotid atherosclerosis) 6 , following surgery 7 , or following haemodialysis 41 . Further investigation around what causes this dysregulation in vivo and what role it may play in disease will be valuable.…”

“…The role that RBCs play in modulating the activity of immune cells is now beginning to be reported 1,4,17 and a small number of papers have outlined how RBCs can become dysregulated in inflammatory conditions or following long term storage 7,20,21 . Our study aimed to build on these initial reports of RBCs derived from either healthy or disease cohorts by intentionally manipulating RBCs through co-culture with cancer cells.…”

“…RBCs isolated from participants with carotid atherosclerosis differ from RBCs isolated from healthy individuals in that they have an attenuated capacity to protect T-cells from apoptosis 6 . In addition, following hip arthroplasty RBCs were less able to stimulate T-cell proliferation when compared to pre-surgery samples 7 .…”

Decreased erythrocyte CD44 and CD58 expression link e-waste Pb toxicity to changes in erythrocyte immunity in preschool children

“…Therefore, erythrocyte CD44 and tumor cell surface hyaluronan may play a key role in erythrocyte adhesion to tumor cells and consequent development of inflammation and tumor metastasis. CD58, also known as lymphocyte functionassociated antigen-3 (LFA-3), is the natural ligand of the lymphocyte receptor CD2, which together forms the main costimulatory pathway for CD28 − CD8 + T cells in humans (Leitner et al, 2015;Yu et al, 2014). In particular, CD58 interacts with CD2 on the lymphocyte membrane to induce T lymphocytes to secrete cytokines, indirectly promoting T cell proliferation and differentiation (Antunes et al, 2011;Shivam et al, 2013).…”