Clinical impact of circulating microRNAs as blood-based marker in childhood acute lymphoblastic leukemia

Swellam, Menha; El-Khazragy⃰, Nashwa

doi:10.1007/s13277-016-4948-7

Cited by 28 publications

(23 citation statements)

References 24 publications

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“…19 Regarding the prognosis, a high level of miR-155 is a predictor of a bad prognosis in patients with CLL 8 and AML. [20][21][22][23] miR-155 has a clear association with lymphomas and leukemias pathogenesis, progression, and response to treatment. At the same time, it has a direct relation with the immune response to HCV infection and its replication and response to treatment as well.…”

Section: Introductionmentioning

confidence: 99%

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In vitro knock‐out of miR‐155 suppresses leukemic and HCV virus loads in pediatric HCV‐4–associated acute lymphoid leukemia: A promising target therapy

Hassan

El-Khazragy⃰

Elshimy

et al. 2019

J of Cellular Biochemistry

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Hepatitis C virus (HCV) infection is a major public health problem, having a high prevalence in Egypt. Leukemia and lymphoma have been associated with HCV infection. MicroRNA‐155 (miR‐155) has been reported to play a regulatory role in cancer, inflammation, and immune response to infection. The expression level of miR‐155 in HCV viremic patients is controversial; although high miR‐155 levels were demonstrated in HCV genotypes 1,2, and 3, low levels of miR‐155 were detected in Egyptian patients with HCV genotype 4. Several studies have investigated the correlation between the levels of miRNA‐155 and the replication of HCV, others have evaluated miRNA‐155 as a prognostic biomarker in different types of cancer. No studies have investigated the impact of miRNA‐155 knockdown on HCV pediatric patients associated with childhood acute lymphoblastic leukemia (ALL). We knocked‐out the miR_155a in cultured polymorphonuclear cells (PBMCs) obtained from 60 children with ALL; 30 were associated with HCV‐4 infection and 30 were HCV negative. The miR_155a, HCV viral load, and cell proliferation werre assessed in treated and untreated cells using TaqMan assay quantitative polymerase chain reaction. We found that miRNA‐155 was significantly upregulated by seven folds in the HCV‐4 associated ALL group; while being linked to high HCV viral load and leukemic burden, miR_155a knock‐out can improve the disease outcome. We conclude that miR‐155 is a critical miRNA that is considered a therapeutic target in pediatric HCV leukemic patients.

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Section: Introductionmentioning

confidence: 99%

“…Patients who achieved complete remission showed low levels of miR‐155 in the plasma . Regarding the prognosis, a high level of miR‐155 is a predictor of a bad prognosis in patients with CLL and AML …”

Section: Introductionmentioning

confidence: 99%

In vitro knock‐out of miR‐155 suppresses leukemic and HCV virus loads in pediatric HCV‐4–associated acute lymphoid leukemia: A promising target therapy

Hassan

El-Khazragy⃰

Elshimy

et al. 2019

J of Cellular Biochemistry

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“…The characteristics of the studies included are shown in (Table 1). 16,17,19–24,28,29,35–44 A total of 1,389 haematological cancer patients (433 leukaemia, 391 lymphomas and 565 MM) and 1013 healthy controls were investigated. In all studies, quantitative real-time chain reaction polymerase (qRT-PCR) tests were used to detect expression levels of miRNAs in either plasma (n=28) or serum (n=22).…”

Section: Resultsmentioning

confidence: 99%

<p>Circulating microRNA as diagnostic biomarkers for haematological cancers: a systematic review and meta-analysis</p>

Drokow

Sun

Ahmed

et al. 2019

CMAR

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Purpose: Recent studies have validated microRNAs (miRNAs) as a diagnostic biomarker for haematological cancers. This study aimed to estimate the overall diagnostic accuracy of circulating miRNAs in haematological malignancies. Materials and Methods: Multiple databases (Google Scholar, PubMed, EMBASE, Cochrane Library,) were searched until 19 th August 2017. Results: The meta-analysis included 50 studies from 20 publications. The diagnostic accuracy was assessed by pooled specificity, sensitivity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under the curve area (AUC) by random effect model. We used QUADAS (Quality Assessment for diagnostic accuracy studies) to evaluate the quality of the included studies. To perform the meta-analysis, we used Meta-Disk 1.4, Revman 5.3 and Stata 12.0 software. High diagnostic accuracy was demonstrated, with a sensitivity of 0.81, a specificity of 0.85, a PLR of 5.28, an NLR of 0.22, a DOR of 30.39, and an AUC of 0.91. Subgroup analyses showed better outcomes for the African population, combined miRNAs and leukaemia patients compared with other subgroups. Conclusion: Our results indicated that circulating miRNAs especially combined miRNA can be used as a diagnostic marker in haematological cancers.

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“…In ALL, a few circulating miRNAs were recently described, and among them miR-146a. Significant higher median levels of miR-100, miR-196a, and miR-146a were reported in blood samples of affected children compared to controls, but the diagnostic efficacy for miR-146a analysis presented superior sensitivity and specificity [ 211 ]. These same authors recently reported the significant increased expression of circulating miR-125b-1 and low levels of miR-203 in serum samples from untreated newly diagnosed children with ALL ( n = 43), as detected by quantitative RT-PCR analysis [ 212 ].…”

Section: Mirnas In Clinicsmentioning

confidence: 99%

MiRNA Dysregulation in Childhood Hematological Cancer

Oliveira

Roberto

Baroni

et al. 2018

IJMS

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For decades, cancer biology focused largely on the protein-encoding genes that have clear roles in tumor development or progression: cell-cycle control, apoptotic evasion, genome instability, drug resistance, or signaling pathways that stimulate growth, angiogenesis, or metastasis. MicroRNAs (miRNAs), however, represent one of the more abundant classes of cell modulators in multicellular organisms and largely contribute to regulating gene expression. Many of the ~2500 miRNAs discovered to date in humans regulate vital biological processes, and their aberrant expression results in pathological and malignant outcomes. In this review, we highlight what has been learned about the roles of miRNAs in some of the most common human pediatric leukemias and lymphomas, along with their value as diagnostic/prognostic factors.

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Clinical impact of circulating microRNAs as blood-based marker in childhood acute lymphoblastic leukemia

Cited by 28 publications

References 24 publications

In vitro knock‐out of miR‐155 suppresses leukemic and HCV virus loads in pediatric HCV‐4–associated acute lymphoid leukemia: A promising target therapy

In vitro knock‐out of miR‐155 suppresses leukemic and HCV virus loads in pediatric HCV‐4–associated acute lymphoid leukemia: A promising target therapy

<p>Circulating microRNA as diagnostic biomarkers for haematological cancers: a systematic review and meta-analysis</p>

MiRNA Dysregulation in Childhood Hematological Cancer

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