Clinical Importance of the Human Umbilical Artery Potassium Channels

Lorigo, Margarida; Oliveira, Nelson Fernandes de

doi:10.3390/cells9091956

Cited by 30 publications

(12 citation statements)

References 215 publications

(387 reference statements)

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“…The opening of K + channels, which leads to cell membrane hyperpolarization by promoting intracellular K + outflow, is a way to decrease intracellular Ca 2+ concentration and induce vasodilation. To date, four types of K + channels with different activation mechanisms have been identified: these are the K V , K Ca , K ir , and K ATP channels ( Lorigo et al, 2020 ). These channels inhibit the activation of VDCCs on the cell membrane, thus reducing extracellular Ca 2+ influx.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Allicin on Acute Myocardial Infarction in Rats via Hydrogen Sulfide-mediated Regulation of Coronary Arterial Vasomotor Function and Myocardial Calcium Transport

Cui

Liu

et al. 2022

Front. Pharmacol.

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Acute myocardial infarction (AMI) is a condition with high morbidity and mortality, for which effective treatments are lacking. Allicin has been reported to exert therapeutic effects on AMI, but the underlying mechanisms of its action have not been fully elucidated. To investigate this, a rat model of AMI was generated by ligating the left anterior descending branch of the coronary artery. DL-propargylglycine (PAG), a specific hydrogen sulfide (H2S) synthetase inhibitor, was used to examine the effects of allicin on H2S production. Isolated coronary arteries and cardiomyocytes were assessed for vascular reactivity and cellular Ca2+ transport using a multiwire myography system and a cell-contraction-ion detection system, respectively. Allicin administration improved cardiac function and myocardial pathology, reduced myocardial enzyme levels, and increased H2S and H2S synthetase levels. Allicin administration resulted in concentration-dependent effects on coronary artery dilation, which were mediated by receptor-dependent Ca2+ channels, ATP-sensitive K+ channels, and sarcoplasmic reticulum (SR) Ca2+ release induced by the ryanodine receptor. Allicin administration improved Ca2+ homeostasis in cardiomyocytes by increasing cardiomyocyte contraction, Ca2+ transient amplitude, myofilament sensitivity, and SR Ca2+ content. Allicin also enhanced Ca2+ uptake via SR Ca2+-ATPase and Ca2+ removal via the Na+/Ca2+ exchanger, and it reduced SR Ca2+ leakage. Notably, the protective effects of allicin were partially attenuated by blockade of H2S production with PAG. Our findings provide novel evidence that allicin-induced production of H2S mediates coronary artery dilation and regulation of Ca2+ homeostasis in AMI. Our study presents a novel mechanistic insight into the anti-AMI effects of allicin and highlights the therapeutic potential of this compound.

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Allicin on Acute Myocardial Infarction in Rats via Hydrogen Sulfide-mediated Regulation of Coronary Arterial Vasomotor Function and Myocardial Calcium Transport

Cui

Liu

et al. 2022

Front. Pharmacol.

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“…K ATP is designed to have the ability to decrease its activity when the intracellular levels of ATP increase. This activity can also be modulated by ATP-independent signaling pathways [ 51 ]. In a study carried out with labdane-302, a diterpene isolated from Xylopia langsdorfian A. St.-Hil.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lippia alba (Mill.) N.E. Brown Essential Oil on the Human Umbilical Artery

Borges

Bastos

Dantas

et al. 2022

Plants

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Lippia alba is popularly known as lemon balm, with its essential oil (EO) cited for displaying antimicrobial, sedative, and vasorelaxant effects. Yet, its action on isolated human vessels has not been described in the literature. Thus, we evaluated the vasorelaxant effect of essential oil of L. alba (EOLa) on human umbilical arteries (HUA) isolated in organ baths. HUA rings were isolated, subjected to contractions induced by potassium chloride (KCl), serotonin (5-HT), or histamine (HIST) to record the isometric tension, and then treated with EOLa (30–1000 µg/mL). The EOLa showed a more prominent inhibitory effect on the pharmacomechanical coupling contraction via HIST with an EC50 value of 277.1 ± 8.5 µg/mL and maximum relaxant effect at 600 µg/mL. The addition of tetraethylammonium (TEA) or 4-aminopyridine (4-AP) in HUA preparations did not inhibit EOLa total relaxant effect at 1000 µg/mL. In the presence of gliblenclamide (GLI), the oil relaxed the HUA rings by 90.8% at maximum concentration. The EOLa was also investigated for its effects on voltage-operated calcium channels (VOCCs), where the HUA preincubation with this oil at 1000 μg/mL inhibited BaCl2 (0.1–30 mM)-induced contractions. This study demonstrates for the first time that EOla has a vasorelaxant effect on HUA and its particular blockade of VOCCs.

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“…Potassium channels have an important role in the regulation of vascular tone of HUAs (Lorigo et al 2020). The functional presence of K v and BK Ca , as well as their involvement in HUA vasoactivity, is well described, while on the other hand, there is not enough information to clearly confirm the existence of K ATP channels in HUA (Sadanaga et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery

Đukanović

Bojic

Marinković

et al. 2022

Can. J. Physiol. Pharmacol.

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Carvacrol is the main compound of essential oils extracted primarily from Thymus and Origanum species. Its various biological activities were confirmed: antioxidant, anti-inflammatory, antibacterial, antifungal, anti-tumour, antinematodal and vasorelaxant action. Although vasodilation mediated by carvacrol was previously described, the exact mechanism of its action has not yet been established. Hence, the aim of this study was to investigate carvacrol vasoactivity on human umbilical arteries (HUA) and different pathways involved in its mechanism of action using tissue bath methodology. Carvacrol caused a significant decrease in vascular tension of 5-HT-pre-contracted umbilical arteries, with EC50 of 442.13 ± 33.8 µM (mean ± standard error of the mean - SEM). At 300 µM, carvacrol shifted downward the 5-HT concentration-response curve with statistical significance of p < 0.001 obtained for the four highest concentrations. At concentration of 1 mM, carvacrol completely abolished BaCl2-induced contraction in Ca2+-free Krebs-Ringer bicarbonate solution (p < 0.001). Isopentenyl pyrophosphate, the antagonist of TRPV3 channel, was able to decrease the efficacy of carvacrol (p < 0.001). The vasorelaxant effect of carvacrol seems to involve the blocking of L-type of Ca2+ channels on smooth muscle cells. However, the role of TRPV3 channels in carvacrol-induced vasodilation of HUA cannot be excluded either.

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Clinical Importance of the Human Umbilical Artery Potassium Channels

Cited by 30 publications

References 215 publications

Protective Effects of Allicin on Acute Myocardial Infarction in Rats via Hydrogen Sulfide-mediated Regulation of Coronary Arterial Vasomotor Function and Myocardial Calcium Transport

Protective Effects of Allicin on Acute Myocardial Infarction in Rats via Hydrogen Sulfide-mediated Regulation of Coronary Arterial Vasomotor Function and Myocardial Calcium Transport

Effect of Lippia alba (Mill.) N.E. Brown Essential Oil on the Human Umbilical Artery

Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery

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