Clinical Investigation of Matrix Metalloproteinases, Tissue Inhibitors of Matrix Metalloproteinases, and Matrix Metalloproteinase/Tissue Inhibitors of Matrix Metalloproteinase Complexes and Their Networks in Apical Periodontitis

Martinho, Frederico Canato; Teixeira, Flávia F.C.; Cardoso, Flávia G.R.; Ferreira, Nádia; Nascimento, Gustavo G.; Carvalho, Cláudio Antônio Talge; Valera, Márcia Carneiro

doi:10.1016/j.joen.2016.04.001

Cited by 39 publications

(55 citation statements)

References 39 publications

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“…()Dentsply–Mai.152 mm past WL602After Instrumenting14 daysELISA––––Martinho et al . ()Dentsply‐Mai.152 mm past WL601After Instrumenting–ELISA––––Sette‐Dias et al . ()––2 mm past WL602After Instrumenting14 daysqPCRTRIzol (−µL)10–25 at 12 000 g Zhi et al .…”

Section: Resultsmentioning

confidence: 99%

A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

et al. 2019

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Aim The primary aim was to identify techniques used to sample and analyse periradicular tissue fluid (PTF) in permanent teeth diagnosed with apical disease during root canal treatment. Secondly, to identify the types of inflammatory mediators studied using this approach. Methodology Data Sources: PubMed, EMBASE, Cochrane Library, Science Direct, Web of Science and OpenGrey. Eligibility Criteria: Clinical studies published until 1 June 2018 which utilized orthograde techniques to sample and analyse PTF were included. Cell culture, laboratory or animal studies and those concerned with investigating inflammatory mediator activity from within healthy or diseased pulp tissue, and not periradicular tissues, were excluded. Study appraisal and methods: In accordance with PRISMA guidelines, data were extracted on study characteristics, target mediators, sampling and assay techniques and the parameters associated with the PTF sampling and eluting protocol. A qualitative synthesis was conducted, and studies were critically appraised using a modified version of the Cochrane risk of bias tool. Results Study Characteristics: From 251 studies, 33 were eligible for inclusion. Sampling techniques included the use of paper points (n = 27), fine needle aspiration (n = 4) and filter strips (n = 2). Assay techniques included enzyme‐linked immunosorbent assay (n = 18), quantitative polymerase chain reaction (n = 9), radioimmunoassay (n = 4), colorimetric assay (n = 2), immunofluorometric assay (n = 1) and cytometric bead array (n = 1). Forty‐five different inflammatory mediators were targeted at the proteomic/metabolomic (n = 25) or transcriptomic level (n = 9). Limitations: Significant heterogeneity exists within the methodology, and only 5 studies disclosed unambiguous information about their PTF sampling and eluting protocols. Conclusions Paper points and proteomic/metabolomic analysis are currently the preferred methods for studying and analysing PTF during root canal treatment. The most studied analytes were IL‐1β and TNF‐α. Implications: Further research is required to develop an optimized PTF sampling and eluting protocol to overcome methodological heterogeneity, and future studies are advised to follow a standardized approach to reporting their methodology.

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Section: Resultsmentioning

confidence: 99%

A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

et al. 2019

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“…), periapical interstitial fluid (Martinho et al . ), root canal exudate (Wahlgren et al . ) and gingival crevicular fluid (Baeza et al .…”

Section: Discussionmentioning

confidence: 99%

“…, Martinho et al . ). Previous studies have revealed that MMPs are involved in apical periodontitis in the development of clinical features by interacting with complex networks (Buzoglu et al .…”

Section: Introductionmentioning

confidence: 97%

Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis

Aksoy¹,

Savtekin

Şehirli

et al. 2019

Int Endodontic J

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Aim To investigate the possible therapeutic effects of alpha‐lipoic acid (ALA) in a model of chronic apical periodontitis in rats by analysing biochemical, histopathological and micro‐CT parameters. Methodology The study was approved by the Animal Ethics Committee of the Near East University. Thirty‐two Wistar rats were divided into four groups of eight rats each: Control Group; ALA Group; AP Group; AP + ALA Group. In the AP and AP + ALA groups, the pulp chambers of the mandibular first molars were surgically exposed and were left open to the oral environment for 4‐weeks to allow the establishment of periapical lesions. The rats in the Control and AP groups were treated intraperitoneally with saline solution (with a daily dose of 100 mg kg−1, for 28 days after periapical lesion induction). The rats in the ALA and AP + ALA groups were treated intraperitoneally with ALA (with a daily dose of 100 mg kg−1, for 28 days after periapical lesion induction). After decapitation, the trunk blood was collected for the assessment of biochemical parameters. The mandibles were surgically removed and dissected for histopathologic analysis and further scanned with micro‐CT. Groups of data were compared with a two‐way analysis of variance (two‐way anova) followed by Sidak's multiple comparison tests. Values of P < 0.05 were regarded as significant. Results TNF‐α, IL‐1β, MMP‐1, MMP‐2 levels were significantly lower in AP + ALA group compared with AP group (P < 0.05). There was a significant difference between the AP and AP + ALA groups according to assessment of the inflammatory scores (P < 0.05). The periapical inflammatory infiltrates were significantly more severe (P < 0.05) in the AP group. The AP + ALA group exhibited lower values both in terms of surface area and volume of resorption cavities than the AP group and this difference was significant (P < 0.05). Conclusion alpha‐lipoic acid treatment provided therapeutic effects on the inhibition of periapical bone loss.

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“…, Martinho et al . ). Hence, the number of patients was set to be 30 per group, which is a sufficient number for a first tentative statistical analysis on continuous data (Krithikadatta ).…”

Section: Methodsmentioning

confidence: 97%

Safety assessment of an etidronate in a sodium hypochlorite solution: randomized double‐blind trial

et al. 2019

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Aim To assess whether Dual Rinse HEDP, an etidronate that can be combined with NaOCl to create an endodontic irrigating solution containing both hypochlorite and a chelator in the form of 1‐hydroxyethane 1,1‐diphosphonic acid (HEDP), alters the clinical efficacy of NaOCl or adds any untoward clinical effects. Methodology In this randomized controlled double‐blind single‐centre trial, a pure NaOCl solution was compared to a HEDP‐containing counterpart regarding antimicrobial efficacy, postoperative pain, and the host response by means of changes in MMP‐9 levels in periapical fluid. Sixty patients presenting with asymptomatic apical periodontitis (one tooth each) were randomly divided into two groups (N = 30) based on irrigation regime. Pre‐ and post‐treatment microbial aerobic and anaerobic cultures and MMP‐9/total protein (TP) periapical fluid samples were collected. Postoperative pain levels were assessed 24 h after treatment. Categorical data were compared between groups using the Fisher's exact test, continuous data using the Wilcoxon signed‐rank test, α = 0.05. Results Irrigation with pure NaOCl rendered 40% canals free of culturable microorganisms, compared to 50% with the NaOCl/HEDP mixture (P = 0.60). As assessed by matrix‐assisted laser desorption/ionization‐time‐of‐flight analysis (MALDI‐TOF), no apparent selection of aerobic or anaerobic taxa occurred in either group. One patient in the NaOCl group experienced moderate pain, whilst two patients in the NaOCl/HEDP group experienced mild postoperative pain. MMP‐9/TP levels in periapical fluid declined significantly (P < 0.001) after 1 week with no medication in the root canal, without significant difference between treatment groups (P > 0.05). Conclusions This trial found no influence of HEDP on clinical NaOCl effects.

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Clinical Investigation of Matrix Metalloproteinases, Tissue Inhibitors of Matrix Metalloproteinases, and Matrix Metalloproteinase/Tissue Inhibitors of Matrix Metalloproteinase Complexes and Their Networks in Apical Periodontitis

Abstract: MMPs, TIMPs, and their complexes (MMP/TIMP) are involved in apical periodontitis by interacting with complex networks in the development of clinical features and the severity of bone destruction.

Cited by 39 publications

References 39 publications

A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis

Safety assessment of an etidronate in a sodium hypochlorite solution: randomized double‐blind trial

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