Clinical isolates of<i>Pseudomonas aeruginosa</i>from superficial skin infections have different physiological patterns

Buivydas, Andrius; Pasanen, Tanja; Senčilo, Ana; Daugelavičius, Rimantas; Vaara, Martti; Bamford, Dennis H.

doi:10.1111/1574-6968.12148

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…Among the microorganisms studied, P. aeruginosa is a pathogenic Gram-negative bacterium responsible for pneumonia 76 and for infections of the urinary tract 77 , gastrointestinal tissue 78 , skin and soft tissues 79 – 81 and is very common in patients with cystic fibrosis 82 . Like other bacteria, P. aeruginosa is becoming resistant to common antibiotics 82 , and AMPs have been proposed as an alternative treatment to combat such infections 83 .…”

Section: Discussionmentioning

confidence: 99%

Structure-function-guided exploration of the antimicrobial peptide polybia-CP identifies activity determinants and generates synthetic therapeutic candidates

et al. 2018

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Antimicrobial peptides (AMPs) constitute promising alternatives to classical antibiotics for the treatment of drug-resistant infections, which are a rapidly emerging global health challenge. However, our understanding of the structure-function relationships of AMPs is limited, and we are just beginning to rationally engineer peptides in order to develop them as therapeutics. Here, we leverage a physicochemical-guided peptide design strategy to identify specific functional hotspots in the wasp-derived AMP polybia-CP and turn this toxic peptide into a viable antimicrobial. Helical fraction, hydrophobicity, and hydrophobic moment are identified as key structural and physicochemical determinants of antimicrobial activity, utilized in combination with rational engineering to generate synthetic AMPs with therapeutic activity in a mouse model. We demonstrate that, by tuning these physicochemical parameters, it is possible to design nontoxic synthetic peptides with enhanced sub-micromolar antimicrobial potency in vitro and anti-infective activity in vivo. We present a physicochemical-guided rational design strategy to generate peptide antibiotics.

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Section: Discussionmentioning

confidence: 99%

Structure-function-guided exploration of the antimicrobial peptide polybia-CP identifies activity determinants and generates synthetic therapeutic candidates

et al. 2018

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“…The fluorescence imaging procedure also has inherent limitations, namely the need for darkness during imaging and a limited depth of excitation (~1.5 mm) for detection of subsurface bacteria. However, as PA tends to be a surface or immediately sub-surface pathogen [69,70], this poses less of a limitation for the detection of PA than it may be for other pathogens.…”

Section: Limitationsmentioning

confidence: 99%

Rapid Diagnosis of Pseudomonas aeruginosa in Wounds with Point-Of-Care Fluorescence Imaing

2021

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Pseudomonas aeruginosa (PA) is a common bacterial pathogen in chronic wounds known for its propensity to form biofilms and evade conventional treatment methods. Early detection of PA in wounds is critical to the mitigation of more severe wound outcomes. Point-of-care bacterial fluorescence imaging illuminates wounds with safe, violet light, triggering the production of cyan fluorescence from PA. A prospective single blind clinical study was conducted to determine the positive predictive value (PPV) of cyan fluorescence for the detection of PA in wounds. Bacterial fluorescence using the MolecuLight i:X imaging device revealed cyan fluorescence signal in 28 chronic wounds, including venous leg ulcers, surgical wounds, diabetic foot ulcers and other wound types. To correlate the cyan signal to the presence of PA, wound regions positive for cyan fluorescence were sampled via curettage. A semi-quantitative culture analysis of curettage samples confirmed the presence of PA in 26/28 wounds, resulting in a PPV of 92.9%. The bacterial load of PA from cyan-positive regions ranged from light to heavy. Less than 20% of wounds that were positive for PA exhibited the classic symptoms of PA infection. These findings suggest that cyan detected on fluorescence images can be used to reliably predict bacteria, specifically PA at the point-of-care.

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“…In addition to being associated with leg ulcers, P. aeruginosa is one of the leading causes of morbidity and mortality in burn victims. 28 The antimicrobial resistance and susceptibility pattern of the organisms isolated on the palmar surfaces is also crucial, as these organisms can cause serious infections upon entering the bloodstream. Listeriosis, a rare but severe foodborne disease, is caused by Listeria monocytogenes, another organism isolated in this study.…”

Section: Discussionmentioning

confidence: 99%

Hand Washing Practices and Antibiotic Susceptibility of Palmar Bacterial Flora in a Tertiary Education Institution in Nigeria

Akinwumi,

Ifeyinwa Okorafor,

Ukatu

et al. 2024

EMJ Microbiol Infect Dis

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Introduction: Hand hygiene is one of the most effective and inexpensive means of preventing the spread of communicable diseases. Rates of handwashing worldwide are low, and poor handwashing practices in universities remain a public health challenge. Objective: The objective of this study was to examine the practice of handwashing, the microbial communities, and the susceptibility pattern of micro-organisms isolated from the palms of students of the Obafemi Awolowo University in Ile-Ife, Osun, Nigeria. Methods: A combination of qualitative and quantitative methods was used to retrieve data. A self-administered questionnaire was utilised to gather socio-demographic characteristics and the practice scale of handwashing from the respondents. Isolation and identification were carried out by culture-based surveys and biochemical tests. Disk diffusion was used to determine susceptibility. Results: The majority of respondents were between ages 21–25 years, with 54.3% of them being female. About half of individuals never used an alcohol-based hand sanitiser, while only 4.9% of respondents admitted to always using soap to wash their hands. Presumptive identification of the organisms showed 38.1% of organisms as Staphylococcus epidermidis and 17.58% as Micrococcus spp. None of the antibiotics showed total efficacy. The resistance to chloramphenicol was low, and <50% of the isolates showed resistance to tetracycline, novobiocin, and sulphonamide. Resistance to nalidixic acid was seen in 58.4% of organisms tested, and 77.75% were susceptible to tetracycline. Conclusion: Instances of improper hand hygiene were high, and this may increase the spread of micro-organisms through hand carriage. High resistance to the antibiotics tested was prevalent. Local trends of antimicrobial resistance must be robustly studied and proper interventions developed.

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Clinical isolates ofPseudomonas aeruginosafrom superficial skin infections have different physiological patterns

Cited by 6 publications

References 24 publications

Structure-function-guided exploration of the antimicrobial peptide polybia-CP identifies activity determinants and generates synthetic therapeutic candidates

Structure-function-guided exploration of the antimicrobial peptide polybia-CP identifies activity determinants and generates synthetic therapeutic candidates

Rapid Diagnosis of Pseudomonas aeruginosa in Wounds with Point-Of-Care Fluorescence Imaing

Hand Washing Practices and Antibiotic Susceptibility of Palmar Bacterial Flora in a Tertiary Education Institution in Nigeria

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