Clinical Isolates of<i>Staphylococcus intermedius</i>Masquerading as Methicillin-Resistant<i>Staphylococcus aureus</i>

Pottumarthy, Sudha; Schapiro, Jeffrey M.; Prentice, Jennifer L.; Houze, Yolanda B.; Swanzy, Susan R.; Fang, Ferric C.; Cookson, Brad T.

doi:10.1128/jcm.42.12.5881-5884.2004

Cited by 81 publications

(76 citation statements)

References 19 publications

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“…If such strains are not identified as S. pseudintermedius strains but are identified as S. aureus strains, they could be misidentified as being methicillin-susceptible strains. Such inadequate species identification could lead to suboptimal or inappropriate treat- ment decisions for methicillin-resistant staphylococcal infections (5,23,27). The second reason is the public health issue of whether methicillin-resistant staphylococcal isolates from pet and farm animals are S. aureus strains.…”

Section: Discussionmentioning

confidence: 99%

Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci

et al. 2010

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In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has been no reliable method to distinguish among CoPS species in veterinary clinical laboratories. In the present study, we sequenced the thermonuclease (nuc) genes of staphylococcal species and devised a multiplex-PCR (M-PCR) method for species identification of CoPS by targeting the nuc gene locus. To evaluate sensitivity and specificity, we used this M-PCR method on 374 staphylococcal strains that had been previously identified to the species level by an hsp60 sequencing approach. We could successfully distinguish between S. aureus, S. hyicus, S. schleiferi, S. intermedius, S. pseudintermedius, and S. delphini groups A and B. The present method was both sensitive (99.8%) and specific (100%). Our M-PCR assay will allow the routine species identification of CoPS isolates from various animal species for clinical veterinary diagnosis.

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Section: Discussionmentioning

confidence: 99%

Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci

et al. 2010

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“…S. aureus, S. intermedius, and S. schleiferi have several phenotypic traits in common, and often an abbreviated number of tests are used for routine clinical identification of these species (8,10). An error in an individual, heavily weighted test can result in misidentification and subsequent misinterpretation of susceptibility tests (22). Differentiation of these species has been further confounded by the addition of a newly proposed species, S. pseudintermedius, isolated from dogs and other animals (4).…”

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confidence: 99%

Comparison of Tests To Detect Oxacillin Resistance in Staphylococcus intermedius , Staphylococcus schleiferi , and Staphylococcus aureus Isolates from Canine Hosts

Bemis¹,

Jones²,

Hiatt³

et al. 2006

J Clin Microbiol

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“…To further assess the specificity of these primer sets, we ran both multiplex reactions (1 and 2) using DNAs extracted from 12 additional staphylococcal reference strains (Table 1), including S. intermedius (ATCC 29663), a species that also expresses free coagulase and that may be confused with S. aureus by both phenotypic and molecular identification strategies (11). While the genus control product was amplified from all isolates, no false-positive peaks were observed and all strains were properly identified (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Comparison of Real-Time PCR and Conventional Biochemical Methods for Identification of Staphylococcus lugdunensis

et al. 2009

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Staphylococcus lugdunensis is an aggressive, virulent member of the coagulase-negative staphylococci (CoNS) that is responsible for severe, rapidly progressive skin and soft tissue infections and native valve endocarditis. To facilitate prompt identification and appropriate therapy, we describe here a rapid and robust multiplex real-time PCR assay that is able to definitively distinguish S. lugdunensis from other staphylococci. Using melting curve analysis, the assay also identifies Staphylococcus aureus and CoNS other than S. lugdunensis and determines MecA-dependent resistance to methicillin (meticillin). When applied to a panel of well-characterized staphylococcal reference strains, as well as 165 clinical isolates previously identified by conventional methods, the assay was both sensitive and specific for S. lugdunensis, correctly identifying the reference strain and all 47 S. lugdunensis isolates without inappropriate amplification of other staphylococci. Furthermore, rapid biochemical identification using the WEE-TAB system to detect ornithine decarboxylase activity was found to be unsuitable as an alternative to PCR identification, displaying just 31% sensitivity and 77% specificity when tested on a subset (90 isolates) of the clinical strains. We therefore propose that this simple, accurate PCR approach will allow for the routine and timely identification of S. lugdunensis in the clinical microbiology laboratory.

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Clinical Isolates ofStaphylococcus intermediusMasquerading as Methicillin-ResistantStaphylococcus aureus

Cited by 81 publications

References 19 publications

Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci

Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci

Comparison of Tests To Detect Oxacillin Resistance in Staphylococcus intermedius , Staphylococcus schleiferi , and Staphylococcus aureus Isolates from Canine Hosts

Comparison of Real-Time PCR and Conventional Biochemical Methods for Identification of Staphylococcus lugdunensis

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