Clinical meaning of age-related expression of fecal cytokeratin 19 in colorectal malignancy

Chang, Chun Chao; Yang, Shung Haur; Chien, Chih Cheng; Chen, Shu Hung; Pan, Shiann; Lee, Chia Long; Lin, Chih Ming; Sun, Hsiao Lun; Huang, Chi Cheng; Wu, Yih Yiing; Yang, Ruey Neng; Huang, Chin Chou

doi:10.1186/1471-2407-9-376

Cited by 20 publications

(17 citation statements)

References 36 publications

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“…In the case of chymotrypsin C suppression, cytokeratin 18, keratin 19, and prolyl 4-hydroxylase were upregulated 1.31, 1.28, and 1.55 folds, respectively, whereas lamin A/C and heat shock 70 kDa protein 8 were downregulated to about 60 or 79%, respectively. All of these differentially expressed proteins have been reported to be involved in cancer metastasis [12][13][14][15][16][17][18][19][20][21][22][23]. Although the above proteins are all associated with cancer, they (with the exception of cytokeratin 18) had no close correlation with either upregulation or suppression of chymotrypsin C. The changes at protein levels of cytokeratin 18 were also consistent with its mRNA level, which was verified by quantitative RT-PCR.…”

Section: Chymotrypsin C and Related Proteins Involved In The Aspc-1 Csupporting

confidence: 58%

Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration

Wang¹,

Sha²,

Liu³

et al. 2011

ABBS

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Pancreatic cancer is a malignant cancer with a high mortality rate. The amount of chymotrypsin C in pancreatic cancer cells is only 20% of that found in normal cells. Chymotrypsin C has been reported to be involved in cancer cell apoptosis, but its effect on pancreatic cancer cell migration is unclear. We performed cell migration scratch assays and Transwell experiments, and found that cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed chymotrypsin C, whereas the cell migration ability was upregulated in Aspc-1 cells in which chymotrypsin C was suppressed. Two-dimensional fluorescence differential in gel electrophoresis/mass spectrometry method was used to identify the proteins that were differentially expressed in Aspc-1 cells that were transfected with plasmids to induce either overexpression or suppressed expression of chymotrypsin C. Among 26 identified differential proteins, cytokeratin 18 was most obviously correlated with chymotrypsin C expression. Cytokeratin 18 is expressed in developmental tissues in early stages of cancer, and is highly expressed in most carcinomas. We speculated that chymotrypsin C might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression.

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Section: Chymotrypsin C and Related Proteins Involved In The Aspc-1 Csupporting

confidence: 58%

Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration

Wang¹,

Sha²,

Liu³

et al. 2011

ABBS

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“…It is a protein that in humans is encoded by the KRT19 gene [24,25]. Its expression can be observed in many normal tissues, and it is overexpressed in lung cancer, colorectal cancer and breast cancer [26-29]. SCC-Ag is a sub-fraction of the tumor antigen TA-4, a 48 kDa glycoprotein first isolated by Kato and Torigoe [30].…”

Section: Discussionmentioning

confidence: 99%

“…Cyfra21-1 is a small molecular protein responsible for structural integrity of epithelial cells, which also functions to promote cell growth, adhesion, migration and invasion [34]. High Cyfra21-1 serum titers have been demonstrated to correspond to poor prognosis in patients with non small cell lung cancer and colorectal cancer [21,26,33]. SCC-Ag may be involved in the malignant behavior of squamous cell cancers, functioning in invasion and/or metastasis.…”

Section: Discussionmentioning

confidence: 99%

Preoperative Cyfra21-1 and SCC-Ag serum titers predict survival in patients with stage II esophageal squamous cell carcinoma

et al. 2012

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BackgroundThe purpose of this study is to evaluate the predictive significance of preoperative serum level of cytokeratin 19 fragments (Cyfra21-1) and squamous cell carcinoma antigen (SCC-Ag) after complete resection in patients with stage II esophageal squamous cell carcinoma (ESCC).MethodsBetween 1995 and 2006, a total of 379 patients in stage II ESCC who underwent complete resection were consecutively recruited. Statistical analyses were applied to test the associations between preoperative serum titers of Cyfra21-1 and SCC-Ag, clinicopathological factors and prognoses.ResultsPreoperative high and normal serum level of Cyfra21-1 and SCC-Ag were found in 47.8%, 52.2% and 72.8%, 27.2%, respectively. The 1-, 3-, 5-year overall survival rate for the entire cohort of patients was 95%, 78%, and 56%, respectively. Median overall survival (OS) was 45.3 months longer in patients with low preoperative serum level of Cyfra21-1 (91.9 months) than those with high preoperative serum level of Cyfra21-1 (46.6 months) (P < 0.001). Median OS among patients with SCC-Ag-low level was also longer than those with SCC-Ag-high level (89.7 vs. 63.7 months, P < 0.001), especially for those with stage IIB (P < 0.001). After multivariate analysis, along with pTNM stage, preoperative serum level of Cyfra21-1 and SCC-Ag were independently and significantly predictive factors (P < 0.001, P < 0.001). Furthermore, the five-year survival rate in double-low subset, either-low subset and double-high subset was 100%, 83% and 27%, respectively (P < 0.001).ConclusionsThe preoperative serum level of Cyfra21-1 and SCC-Ag are independently significant predictors which negatively affected the survivals of patients with stage II ESCC.

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“…(16)(17)(18) Together, these studies indicate that newly formed lymphatic vessels are likely to be the major pathway for the spread of tumors to the PNP.In the present study, we used cytokeratin 19 (CK19), (19) a commonly used prognostic marker, to evaluate lymphatic invasion. It has been reported that CK19 is highly expressed in lymph nodes exhibiting disseminated tumor cells, (20) and significant levels of CK19 have been found in the peripheral blood of patients with other malignancies, including liver, breast, and pancreatic cancers. (21,22) Thus, using CK19 as a potential marker for lymphatic invasion of the PNP may both clarify the significance of lymphangiogenesis as a major route for the spread of tumor cells to the nervous system and provide mechanistic information regarding this method of tumor metastasis for further research and clinical application.…”

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confidence: 99%

Analysis of tumor‐induced lymphangiogenesis and lymphatic vessel invasion of pancreatic carcinoma in the peripheral nerve plexus

Peng

Jin

et al. 2012

Cancer Science

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Cancer cells can metastasize throughout the body by various mechanisms, including the lymphatic system, resulting in tumorinduced lymphangiogenesis that can profoundly affect patient survival. The aim of the present study was to examine the role of lymphangiogenesis in the metastasis of pancreatic cancer to the peripheral nerve plexus. Immunohistochemistry was performed to analyze specimens obtained from 70 ductal adenocarcinoma patients. The markers used included lymphangiogenic factor vascular endothelial growth factor (VEGF)-C, the lymphatic-specific marker D2-40, and cytokeratin 19, an independent prognostic factor for pancreatic tumors. The relationship between survival rate and invasion of both the lymphatic vessels and peripancreatic nerve plexus (PNP) was evaluated, with clearly elevated lymphatic vessel density (LVD) in tissues adjacent to the cancer tissues. In fact, LVD levels were higher in adjacent tissues than in localized cancer tissues, and lymphatic vessel invasion into tissues adjacent to the tumor was significantly correlated with both PNP invasion (P = 0.005) and lymph node metastasis (P = 0.010). Correspondingly, LVD in tissues adjacent to the tumor was correlated with both invasion of lymphatic vessels surrounding the tumor (P = 0.024) and VEGF-C expression (P = 0.031); in addition, VEGF-C expression was correlated with invasion of lymphatic vessels around the tumor (P = 0.004). Survival rates were significantly lower in patients in whom there was peritumor lymphatic vessel invasion (P < 0.001), extrapancreatic nerve plexus invasion (P = 0.001), and/or lymph node metastasis (P < 0.001). Based on these results, lymphatic invasion associated with adjacent tumor growth likely contributes to the development of metastatic tumors that invade the

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Clinical meaning of age-related expression of fecal cytokeratin 19 in colorectal malignancy

Cited by 20 publications

References 36 publications

Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration

Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration

Preoperative Cyfra21-1 and SCC-Ag serum titers predict survival in patients with stage II esophageal squamous cell carcinoma

Analysis of tumor‐induced lymphangiogenesis and lymphatic vessel invasion of pancreatic carcinoma in the peripheral nerve plexus

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