Clinical Microbial Identification of Severe Oral Infections by MALDI-TOF Mass Spectrometry in Stockholm County: an 11-Year (2010 to 2020) Epidemiological Investigation

Al‐Manei, Khaled; Ghorbani, Mahin; Al-Manei, Kholod Khalil; Sobkowiak, Michał J.; Lund, Bodil; Hazırolan, Gülşen­; Chen, Margaret Sällberg; Özenci, Volkan

doi:10.1128/spectrum.02487-22

Cited by 9 publications

(7 citation statements)

References 49 publications

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“…These bacteria are well-recognized for causing central nervous system infections, and in recent decades, the incidence of CAs with SAG bacteria has increased [25]. They are often isolated from odontogenic infectious lesions including periapical abscesses, periodontal abscesses, and pericoronitis [26][27][28][29][30]. Also, S. constellatus and S. intermedius found in dental plaque have been associated with the development of periodontal disease [31].…”

Section: Discussionmentioning

confidence: 99%

“…Also, S. constellatus and S. intermedius found in dental plaque have been associated with the development of periodontal disease [ 31 ]. A recent study from Sweden concluded that S. anginosus alone, as a single species, accounted for over 20% of oral bacterial infections [ 30 ], and a previous study showed a very close similarity between brain and oral isolates of SAG in a CA patient with periodontitis, where the use of highly discriminative phenotypic and genetic fingerprint techniques could not exclude that microorganisms involved in CA formation came from the periodontal pocket [ 32 ]. However, it is important to bear in mind that microorganisms from the SAG, especially S. anginosus , are commonly isolated from gastrointestinal and genitourinary tract infections as well [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

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Cerebral abscesses with odontogenic origin: a population-based cohort study

et al. 2023

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Objectives Recent studies have indicated that cerebral abscess (CA) patients with odontogenic origin are on the rise. However, CA patients are often poorly characterized and with an unknown etiologic background. The purpose of this study is to identify and characterize CA patients that may have an odontogenic origin based on microbiologic, radiographic, and/or clinical findings. Materials and methods This is a population-based cohort study analyzing retrospective and prospective data from CA patients. Radiographic examinations of panoramic radiographs (PRs) or computed tomography (CT) scans were conducted. CA patients characterized with odontogenic origin required the fulfilment of the following criteria on admission: (1) Oral pathologic conditions were the only bacterial infections present, (2) oral microorganisms were isolated in the purulent exudate from the brain, and (3) radiographically and/or clinical recordings of oral pathologic conditions. Results A total of 44 patients could be included in this study of which 25 (57%) were characterized as having CA with a likely odontogenic origin. Type two diabetes (T2D) (p = 0.014) and microorganisms of the Streptococcus anginosus group (SAG) (p < 0.01) were overrepresented in patients with CAs of odontogenic origin. Conclusions Odontogenic infections may cause CAs to a greater extent than previously assumed. T2D was overrepresented among patients with odontogenic CA. When microorganisms of the SAG were isolated from the brain pus, CA patients had a predisposing odontogenic or sinus infection. Clinical relevance The identification of patients with a likely odontogenic CA will contribute to understanding the etiology of the infectious disease and highlighting the importance of preserving oral health.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cerebral abscesses with odontogenic origin: a population-based cohort study

et al. 2023

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“…Various phenotypic screening tools based on biochemical properties, such as API ID32C and AuxaColor, are commercially available [33][34][35][36] as well as automated metabolic characterisation facilities, i.e., VITEK 2 and Biolog's Omnilog System [37,38]. Recently, MALDI-TOF mass spectrometry (based on the comparison of protein mass spectra with well-characterised reference strains included in reliable databases) is proving to be a commonly used methodology for microbial (mainly bacterial) identification, especially in the clinical fields but also in commercial sectors [39][40][41].…”

Section: Microbial Resources and Service Supplymentioning

confidence: 99%

Treasures of Italian Microbial Culture Collections: An Overview of Preserved Biological Resources, Offered Services and Know-How, and Management

Moretti,

Tartaglia,

Accotto

et al. 2024

Sustainability

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Microorganisms, microbiomes, and their products (e.g., enzymes, metabolites, antibiotics, etc.) are key players in the functioning of both natural and anthropized Earth ecosystems; they can be exploited for both research purposes and biotechnological applications, including fighting the big challenges of our era, such as climate change. Culture collections (CCs) and microbial Biological Resource Centres (mBRCs) are repositories of microorganisms that investigate and safeguard biodiversity and facilitate the scientific and industrial communities’ access to microbial strains and related know-how by providing external users with skills and services. Considering this, CCs and mBRCs are pivotal institutions for the valorisation of microorganisms, the safeguarding of life, and the fostering of excellent bioscience. The aim of this review is to present the state-of-the-art of Italian CCs and mBRCs, highlighting strengths, weaknesses, threats, and opportunities. Italy is, indeed, a hotspot of microbial biodiversity with a high rate of endemism and incredible potential, not only for the food and beverage sector (i.e., “Made in Italy” products), where microorganisms can have a beneficial or a spoiling function, but also to guarantee environmental sustainability and foster the bioeconomy through the design of new bioprocesses and products. However, weaknesses, such as the lack of management rules in accordance with international quality standards, are also analysed and ways of overcoming them are discussed. In this context, an overview is given of the Joint Research Unit MIRRI-IT and the European-funded SUS-MIRRI.IT project, which aims to improve the management and sustainability of Italian microbial collections, and serves as a starting point for an innovative revolution in the context of CCs and mBRCs worldwide.

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“…Once pulp vitality becomes dysfunctional, the pulp degenerates and may be invaded by microorganisms, eventually leading to total necrosis [ 14 ]. Depending on the host defense response and the degree of microbial virulence and their byproduct, untreated pulpal infection may also spread beyond the root apex, leading to periapical pathosis [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Incidence and influential factors in pulp necrosis and periapical pathosis following indirect restorations: a systematic review and meta-analysis

et al. 2023

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Background Restoring vital teeth with indirect restorations may threaten dental pulp integrity. However, the incidence of and influential factors on pulp necrosis and periapical pathosis in such teeth are still unknown. Therefore, this systematic review and meta-analysis aimed to investigate the incidence of and influential factors on pulp necrosis and periapical pathosis of vital teeth following indirect restorations. Methods The search was conducted in five databases, using MEDLINE via PubMed, Web of Science, EMBASE, CINAHL, and Cochrane Library. Eligible clinical trials and cohort studies were included. The risk of bias was assessed using Joanna Briggs Institute’s critical appraisal tool and Newcastle–Ottawa Scale. The overall incidences of pulp necrosis and periapical pathosis following indirect restorations were calculated using a random effects model. Subgroup meta-analyses were also performed to determine the potential influencing factors for pulp necrosis and periapical pathosis. The certainty of the evidence was assessed using the GRADE tool. Results A total of 5,814 studies were identified, of which 37 were included in the meta-analysis. The overall incidences of pulp necrosis and periapical pathosis following indirect restorations were determined to be 5.02% and 3.63%, respectively. All studies were assessed as having a moderate-low risk of bias. The incidence of pulp necrosis following indirect restorations increased when the pulp status was objectively assessed (thermal/electrical testing). The presence of pre-operative caries or restorations, treatment of anterior teeth, temporization for more than two weeks, and cementation with eugenol-free temporary cement, all increased this incidence. Final impression with polyether and permanent cementation with glass ionomer cement both increased the incidence of pulp necrosis. Longer follow-up periods (> 10 years) and treatment provided by undergraduate students or general practitioners were also factors that increased this incidence. On the other hand, the incidence of periapical pathosis increased when teeth were restored with fixed partial dentures, the bone level was < 35%, and the follow-up was > 10 years. The certainty of the evidence overall was assessed as low. Conclusions Although the incidences of pulp necrosis and periapical pathosis following indirect restorations remain low, many factors affect these incidences that should thus be considered when planning indirect restorations on vital teeth. Database registration PROSPERO (CRD42020218378).

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Clinical Microbial Identification of Severe Oral Infections by MALDI-TOF Mass Spectrometry in Stockholm County: an 11-Year (2010 to 2020) Epidemiological Investigation

Abstract: Oral diseases and associated microbes are a risk factor for systemic diseases and can change the courses of these diseases. To date, epidemiological data on microbial oral infections are scarce, and longitudinal reports are lacking.

Cited by 9 publications

References 49 publications

Cerebral abscesses with odontogenic origin: a population-based cohort study

Cerebral abscesses with odontogenic origin: a population-based cohort study

Treasures of Italian Microbial Culture Collections: An Overview of Preserved Biological Resources, Offered Services and Know-How, and Management

Incidence and influential factors in pulp necrosis and periapical pathosis following indirect restorations: a systematic review and meta-analysis

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