Clinical Outcomes of Preimplantation Genetic Testing (PGT) Application in Couples with Chromosomal Inversion, a Study in the Chinese Han Population

Abstract

Background Chromosomal inversion was considered to have adverse effects on pregnancy outcomes through abnormal gametogenesis. The purpose of this retrospective study was to investigate whether preimplantation genetic testing (PGT) improves pregnancy outcomes for couples with chromosomal inversion. Methods A total of 188 cycles from 165 couples with one chromosomal inversion carrier were divided into two groups: PGT (136 cycles, 125 couples) and non-PGT (52 cycles, 50 couples). Biochemical pregnancy, clinical pregnancy, ongoing pregnancy, miscarriage and live birth rates of their first transfer cycles, as well as cumulative live birth rates of each cycle and euploidy rates, were analyzed. Results There were no statistically significant differences in the pregnancy outcomes between the two groups. The euploidy rate of pericentric inversion carriers was not higher than that of paracentric inversion carriers in PGT group (60.71% vs 50.54%, P=0.073). Similarly, the euploid rate of male carriers was not higher than that of female carriers (61.2% vs 56.1%, P=0.256). Conclusions Due to limitation of retrospective study and small sample size, our current data showed that PGT cannot provide prominent benefits for inversion carriers in the Chinese Han population. Further prospective randomized controlled trials are needed to evaluate the effects of PGT.

Preimplantation genetic testing for aneuploidy failed to improve cumulative live birth rate in patients with limited good-quality embryos: a retrospective cohort study

“…Luteal phase support with 20 mg of oral dydrogesterone (Duphaston, Abbott, USA) twice a day and 200 mg of vaginal progesterone capsules (Utrogestan, Besins Manufacturing, Belgium) once a day was initiated on the day of oocytes retrieval until the 12th week of gestation in most circumstances. For patients who underwent frozen embryo transfer, endometrial preparation and luteal phase support protocols, as well as blastocyst vitrification-thawing and transfer procedures that followed, were completed as described in a previous study (25).…”

Incidence, dynamics and recurrences of reverse cleavage in aneuploid, mosaic and euploid blastocysts, and its relationship with embryo quality

“…The developmental potential of aneuploid embryos is far inferior to that of euploid embryos [9][10][11][12]. Many research groups are now studying the relationship between embryo information obtained by TL technology and embryo ploidy results [13][14][15]. Among these research contents, the abnormal cleavage pattern of embryos is an important one.…”