Clinical outcomes of xeno-free allogeneic cultivated limbal epithelial transplantation for bilateral limbal stem cell deficiency

Basu, Sayan; Fernandez, Mark M.; Das, Sujata; Gaddipati, Subhash; Vemuganti, Geeta K.; Sangwan, Veena

doi:10.1136/bjophthalmol-2012-301869

Cited by 73 publications

(63 citation statements)

References 22 publications

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“…This is in contrast to the success rate of allogenic cultivated limbal epithelial transplantation published in literature which ranges from 50% to 100% in various studies with varied follow-ups. In the study published by Basu et al ,7 the technique was successful for long-term ocular surface restoration in 71.4% of the 28 recipient eyes at mean follow-up of 4.8 years. Authors hypothesise that the success of this technique could be related to the severity of the initial insult at the limbus at the time of the chemical injury.…”

Section: Discussionmentioning

confidence: 88%

“…Bilateral LSCD requires limbal allografting wherein the donor is a person of the same species, either living related or cadaveric 6. Living donors are preferred as limbal cells obtained from cadavers have a lower proliferative rate in vitro and a poorer corneal epithelisation rate in vivo 7. Also, the biopsy can be either used directly on the recipient or may be cultivated in laboratory.…”

Section: Discussionmentioning

confidence: 99%

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Childhood bilateral limbal stem cell deficiency: long-term management and outcome

Jain

Sangwan

2013

BMJ Case Reports

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A 6-year-old boy presented in 1998 with a 1 month history of lime injury in both eyes. Grade 4 chemical injury (Roper-Hall classification) progressed to total limbal stem cell deficiency in both eyes. Between July 2000 and July 2009, he cumulatively underwent 11 limbal stem cell transplantation surgeries, either direct or cultivated from either his father or mother, and 3 corneal transplants in both eyes. Each of the procedures led to a transient clearing of his visual axis to give vision in the range of 20/60 to 20/100 for a period of 1-3 months after which it failed. He finally underwent Boston keratoprosthesis implantation and now enjoys 20/20 vision with stable optic disc and visual field at a follow-up of 41 months. Timely intervention in childhood helped in prevention of amblyopia in this patient. Perseverance by the patient, his relatives and treating ophthalmologist is very important.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Childhood bilateral limbal stem cell deficiency: long-term management and outcome

Jain

Sangwan

2013

BMJ Case Reports

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“…First described by Kenyon and Tseng,2 the surgical technique of limbal transplantation has since evolved from conjunctival-limbal grafting to ex vivo—cultivated limbal epithelial transplantation, introduced by Pellegrini et al 3. Authors have reported results of allogeneic cultivated limbal epithelial transplantation and it can successfully restore the ocular surface and improve vision in patients with corneal blindness owing to bilateral LSCD 4. SLET, recently described by our group involves in vivo expansion of limbal stem cells over the amniotic membrane placed over the surface of the cornea 1.…”

Section: Discussionmentioning

confidence: 99%

Growth of corneal epithelial cells over in situ therapeutic contact lens after simple limbal epithelial transplantation (SLET)

2013

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SUMMARYAn 11-year-old boy underwent simple limbal epithelial transplantation (SLET) from the healthy right eye to his left eye for total limbal stem cell deficiency. One month later, corneal surface epithelialised and whitish plaques overlying the transplants were seen inferiorly. Those plaques were adherent to the surface of the contact lens and underlying corneal surface had smooth elevations. Similar findings were noted in a 23-year man following cyanoacrylate glue application for corneal perforation. On histological and immunohistochemical analysis, cells lining the contact lenses were identified as corneal epithelial cells. These cases illustrate epithelial cell growth on the contact lens and epithelial hyperplasia on corresponding surface of the cornea. Exorbitant proliferation of the epithelial cells may be owing to young age; therefore, early contact lens removal after SLET in young age, can possibly avoid epithelial hyperplasia. This also reiterates the possibility of using contact lens as a scaffold to grow epithelial cells. BACKGROUND

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“…The downsides of allogeneic transplantation include the inherent risks of immune rejection, and the cost and potential complications of lifelong or, at minimum, prolonged immunosuppression. 16 Though allogeneic therapy is still employed in instances of total bilateral disease, as research into cultivated autologous techniques continues, the reliance on allogeneic tissues is likely to decrease.…”

Section: Allogeneic Sctmentioning

confidence: 99%

Autologous Limbal Stem Cell Transplantation: The Progression of Diagnosis and Treatment

Khan-Farooqi

Chodosh

2016

Seminars in Ophthalmology

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Though the corneoscleral limbus has been studied for more than 150 years, our ability to successfully treat those with limbal stem cell deficiency (LSCD) is still a work in progress. Today, the only definitive cure for LSCD is stem cell transplantation. This review briefly discusses the evolution of our understanding of limbal stem cell function, provides an outline of the history of autologous limbal stem cell (LSC) transplantation, and discusses current techniques and future strategies for LSCD treatment.

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Clinical outcomes of xeno-free allogeneic cultivated limbal epithelial transplantation for bilateral limbal stem cell deficiency

Abstract: Allogeneic cultivated limbal epithelial transplantation, followed by PK when needed, can successfully restore the ocular surface and improve vision in patients with corneal blindness due to bilateral LSCD.

Cited by 73 publications

References 22 publications

Childhood bilateral limbal stem cell deficiency: long-term management and outcome

Childhood bilateral limbal stem cell deficiency: long-term management and outcome

Growth of corneal epithelial cells over in situ therapeutic contact lens after simple limbal epithelial transplantation (SLET)

Autologous Limbal Stem Cell Transplantation: The Progression of Diagnosis and Treatment

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