Clinical, Parasitological and Immunological Aspects of Experimental Infection with Trypanosoma evansi in Dogs

Aquino, Lúcia Padilha Cury Thomaz de; Machado, Rosangela Zacarias; Alessi, Antônio Carlos; Marques, Luiz Carlos; Castro, Márcio Botelho de; Malheiros, Euclides Braga

doi:10.1590/s0074-02761999000200025

Cited by 64 publications

(48 citation statements)

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“…Serum was stored at -20 °C. Serum samples obtained in previous studies of experimental T. evansi infection in dogs (AQUINO et al, 1999), bovines (POCHINI, 2000), coatis (HERRERA et al, 2001), and donkeys (CADIOLI, 2001) were used. Sera obtained from the experimentally infected animals before inoculation and on different days during infection were used in Western blotting and as reference negative and positive control sera in serology.…”

Section: Animalsmentioning

confidence: 99%

“…al, 1999). Antigenic substrate for IFAT and soluble antigen used in ELISA and Western blotting were prepared according to Aquino et al (1999). Protein content of the soluble antigen was determined by the bicinconinic acid method (BCA Reagent Kit -Pierce Chemical Company).…”

Section: Antigen Preparationmentioning

confidence: 99%

“…Diagnosis of trypanosomes by direct parasitological techniques is not reliable, specially for naturally occurring field cases, where parasitemias are often low and sporadic (RAE;LUCKINS, 1984;BENGALY;KANWE;DUVALLET, 1995;NANTULYA, 1994). Serological tests for circulating antibody, ELISA and IFAT, have been intensively employed, showing good sensitivity (PAYNE et al, 1991;MUKANI et al, 1992;MONZON;HOYOS;JARA, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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The present research investigated the presence of T. evansi antibodies in animals from the subregion of Nhecolandia, in the Pantanal Sul-mato-grossense, by means of an enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT), and the pattern of polypeptide recognition by sera from experimentally and naturally infected hosts using Western blotting. Serum samples were obtained from bovines (n = 102), horses (n = 98), and dogs (n = 55), and from 32 free-ranging coatis (Nasua nasua). None of the bovines were found positive, while sera from 16 dogs (29%) and 23 horses (23.4%) were positive by ELISA. Sera from 8 coatis (25%) were found positive using IFAT. Western blotting revealed major polypeptides of T. evansi with molecular weight ranging from 74 to 38 kDa. The polypeptides of 66, 48-46, and 38 kDa were identified by sera from experimentally infected bovines, donkeys, dogs, and coatis. The 48-46 and 38 kDa bands were mainly recognized in chronic phase of infection. The antigen with apparent molecular weight of 66 kDa, revealed by antibodies from all experimental animals, was also recognized in sera of horses and dogs from the Pantanal. The 48-46 kDa polypeptide was identified by antibodies from all naturally infected animals and must be further evaluated for use in specific diagnosis of T. evansi infection.Keywords: Trypanosoma evansi, ELISA-test, IFAT, Pantanal mato-grossense, antigenic characterization. ResumoO trabalho de pesquisa investigou a presença de anticorpos anti-T. evansi em animais da sub-região da Nhecolândia, no Pantanal sul-mato-grossense, pelo ensaio imunoenzimático (ELISA) e a reação de imunofluorescência indireta (RIFI). O reconhecimento de polipeptídeos de T. evansi foi realizado pela técnica de "Western blotting", utilizando soros de animais experimentalmente e naturalmente infectados. As amostras de soro foram obtidas de bovinos (n = 102), cavalos (n = 98) e cães (n = 55) e de 32 quatis de vida livre (Nasua nasua) do pantanal mato-grossense. Todos os soros dos bovinos foram negativos, enquanto soros de 16 cães (29%) e 23 cavalos (23,4%) foram positivos pelo ELISA. Soros de oito quatis (25%) foram positivos pela RIFI. Pelo "Western-blotting" foi possível revelar polipeptídeos de T. evansi, com peso molecular variando de 74 a 38 kDa. Os polipeptídeos de 66, 48-46 e 38 kDa foram identificados por soros de bovinos, cavalos, cães e quatis experimentalmente infectados. As bandas de 48-46 e 38 kDa foram reconhecidas principalmente na fase crônica da infecção. O antígeno com peso molecular aparente de 66 kDa, revelado por anticorpos de todos os animais experimentais, também foi reconhecido por soros de cavalos e cães do Pantanal. O polipeptídeo de 48-46 kDa foi identificado por anticorpos de todos os animais naturalmente infectados, devendo ser avaliado para o diagnóstico específico da infecção pelo T. evansi.

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Section: Animalsmentioning

confidence: 99%

Section: Antigen Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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“…A doença causada por Trypanosoma evansi é comumente denominada surra (Hoare 1972), derrengadera (Hoare 1972, Levine 1973, "mal das cadeiras" (Silva et al 1995a, Aquino et al 1999) ou "peste quebra-bunda" (Hoare 1972), dependendo do local do mundo onde ocorre. No passado, T. evansi foi também denominado T. equinum, T. hippicum e T. venezuelense (Hoare 1972).…”

Section: Introdução Introdução Introdução Introdução Introduçãounclassified

“…No Brasil, T. evansi afeta principalmente eqüinos e a prevalência da infecção varia de região para região (Dávila & Silva 2000, Herrera et al 2004. A doença é enzoótica em eqüinos do Pantanal matogrossense, onde assume importância econômica devido à grande população de eqüinos (49.000) da região (Silva et al 1995a, Aquino et al 1999. Ali, a tripanossomíase por T. evansi é também descrita em cães (Nunes et al 1994, Silva et al 1995b, Herrera et al 2004, capivaras (Hydrochaeris hydrochaeris) (Nunes et al 1993, Herrera et al 2004, quatis (Nasua nasua) (Nunes & Oshiro 1990), bovinos, búfalos, pequenos marsupiais (por exemplo, guaiaquicas [Monodelphis spp]) e tatus (Dasypus spp) (Herrera et al 2004).…”

Section: Introdução Introdução Introdução Introdução Introduçãounclassified

Surtos de tripanossomíase por Trypanosoma evansi em eqüinos no Rio Grande do Sul: aspectos epidemiológicos, clínicos, hematológicos e patológicos

et al. 2005

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Additionally, around 80 mares were sent to Farm A to be bred. Of those, 66 became ill and 56 died after being returned to their farms of origin. Twenty one horses clinically affected by the disease were observed. Clinical signs included loss of weight (despite voracious appetite), lethargy, incoordination and instability of hindlimbs, atrophy of the large muscles of the hindlimbs, muscle weakness and paleness of mucosae. Specimens of T. evansi were detected in the blood drawn from four affected horses. Normocytic normochromic anemia with PCVs ranging from 15 to 31%, leucocytosis due to lymphocytosis associated to large atypical lymphocytes was observed in several affected horses. High levels of antibodies against T. evansi were detected in the serum of six horses from Farm A. Eight horses presented encephalic neurological signs such as circling, ataxia, blindness, excitation, falls, listlessness, proprioception deficits and head tilt. One horse assumed a "dogseating position". Necropsy findings included muscle atrophy, enlargement and lymphoid hyperplasia of the spleen and lymphnodes, edema and softening of the white and grey matter of the brain. Histologically, an overwhelming necrotizing panencephalitis was observed in the seven horses with encephalic signs. This panencephalitis was characterized by marked edema, demyelination and necrosis and perivascular infiltrates of 6-10 layers of lymphocytes and plasm cells affecting both the white and gray matter. Several plasm cells in the inflammatory infiltrate contained numerous eosinophilic globules in their cytoplasm (Mott cells). Similar histological lesions were observed in the spinal cord of the horse with the "dog-seating position". The brains of five horses with the encephalic signs were submitted to immunohistochemistry stain by the streptavidinbiotin technique. In all of those five brains moderate to abundant specimens of T. evansi in the perivascular spaces and neuropile were marked by the specific antibody. Epidemiological, clinical, hematological, and pathological aspects of equine trypanosomiasis caused by T. evansi are discussed.

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Trypanosoma evansi in inbred and Swiss-Webster mice: distinct aspects of pathogenesis

et al. 2004

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Trypanosoma evansi (Trypanosomatidae, Kinetoplastida) is a salivarian trypanosomatid that infects eight mammal orders spread over America, Europe and Asia. In Brazil, T. evansi is the etiological agent of "Mal de Cadeiras", a horse disease very often described in the region known as Pantanal do Mato Grosso. Few data concerning the genetic diversity and biology of subpopulations of T. evansi that circulate in Brazil are available. The factors that modulate the interaction of this parasite with its hosts also remain to be elucidated. Here we evaluated the course of experimental infection of six T. evansi isolates derived from domestic and wild animals in Swiss-Webster mice and three Mus musculus lineages. The follow-up included biological, immunological as well as biochemical and hematological parameters. The same isolates as well as three others were characterized by pulsed-field electrophoresis. Our results showed that T. evansi isolates displayed significant differences regarding behavior and morbidity patterns in the distinct mouse lineages. Nevertheless, these differences could not be correlated with pulsed-field electrophoresis profiles. Indeed, concerning this molecular marker, only microheterogeneity was observed. Moreover, we observed that the outcome of the infection is defined by both host genetic background and peculiarities (virulence factors) of the distinct T. evansi isolates. Anemia and hypoglycemia were the only features that could be observed in all mouse lineages, independently of the inoculated T. evansi subpopulation. In addition, our data also show that Mus musculus is a suitable model host for the study of the different pathogenetic features of T. evansi infection.

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Clinical, Parasitological and Immunological Aspects of Experimental Infection with Trypanosoma evansi in Dogs

Cited by 64 publications

References 15 publications

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Surtos de tripanossomíase por Trypanosoma evansi em eqüinos no Rio Grande do Sul: aspectos epidemiológicos, clínicos, hematológicos e patológicos

Trypanosoma evansi in inbred and Swiss-Webster mice: distinct aspects of pathogenesis

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