Clinical peculiarities of<i>mycoplasma</i>infection in children with community pneumonia

Neamtu, Liuba; Sciuca, Svetlana; Selevestru, Rodica

doi:10.1183/13993003.congress-2016.pa1273

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“…Global outbreaks of M. pneumoniae occur every 3–7 years. The clinical symptoms of M. pneumoniae infection are diverse [3]. Individuals with a mild infection can be free of symptoms.…”

Section: Introductionmentioning

confidence: 99%

The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells

Xue

Zhao

et al. 2019

Bioscience Reports

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Mycoplasma pneumoniae is one of the most common pathogenic causes of community-acquired pneumonia. Hydrogen sulfide, alanine, and pyruvate producing enzyme (HapE) is a recently discovered M. pneumoniae virulence factor that can produce H2S to promote erythrocyte lysis. However, other cytotoxic effects of HapE have not been explored. The present study examined the effects of this enzyme on normal human bronchial epithelial (NHBE) cells, in an attempt to identify additional mechanisms of M. pneumoniae pathogenesis. Recombinant HapE was purified for use in downstream assays. MTT and colony formation assays were conducted to determine the effects of HapE on cell viability and growth, while flow cytometry was used to examine changes in cell proliferation and cell cycle function. ELISA was performed to examine changes in the cytokine profile of HapE-treated cells. HapE treatment arrested NHBE cells in S phase and inhibited cell proliferation in a concentration-dependent manner. The anti-inflammatory factors interleukin (IL)-4 and IL-6 were significantly enhanced following HapE treatment. Increased secretion of pro-inflammatory factors was not observed. The effects of HapE on the respiratory epithelium may have an impact on the efficiency of host immune surveillance and pathogen elimination, and contribute to the pathogenesis of M. pneumoniae.

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“…Global outbreaks of M. pneumoniae occur every 3–7 years. The clinical symptoms of M. pneumoniae infection are diverse [3]. Individuals with a mild infection can be free of symptoms.…”

Section: Introductionmentioning

confidence: 99%

The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells

Xue

Zhao

et al. 2019

Bioscience Reports

View full text Add to dashboard Cite

show abstract

Clinical peculiarities ofmycoplasmainfection in children with community pneumonia

Cited by 1 publication

References 0 publications

The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells

The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells

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