Clinical Performance of an Automated Stool DNA Assay for Detection of Colorectal Neoplasia

Lidgard, Graham P.; Domanico, Michael J.; Bruinsma, Janelle J.; Light, James; Gagrat, Zubin D.; Oldham-Haltom, Rebecca; Fourrier, Keith D.; Allawi, Hatim T.; Yab, Tracy C.; Taylor, William R.; Simonson, Julie A.; Devens, Mary E.; Heigh, Russell I.; Ahlquist, David A.; Berger, Barry M.

doi:10.1016/j.cgh.2013.04.023

Cited by 119 publications

(96 citation statements)

References 15 publications

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“…Calistri et al (6) , and Lidgard et al (15) , also did not find any difference among the different stages suggesting that it may be a screen method for precocious cancer.…”

Section: Discussionmentioning

confidence: 90%

“…The association of DNA alterations shown better results, with a higher cost. A multitarget study of DNA in the stools that measure B-actin, mutant K-ras, the aberrant methylation of the genes BMP3 and NDR4 detected cancer in 98% of the CRC patients, with a specificity of 90% (15) . A recent study found a higher methylated NDRG4 in the stools of patients with CRC, with a. sensitivity and specificity of 76.2 and 89.15% (27) .…”

Section: Discussionmentioning

confidence: 99%

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Human Dna Quantification in the Stools of Patients With Colorectal Cancer

Teixeira

Lima

Souza

et al. 2015

Arq. Gastroenterol.

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-Background -Colorectal cancer is one of the main cause of cancer in the world. Colonoscopy is the best screen method, however the compliance is less than 50%. Quantification of human DNA (hDNA) in the feces may be a possible screen non-invasive method that is a consequence of the high proliferation and exfoliation of cancer cells. Objective -To quantify the human DNA in the stools of patients with colorectal cancer or polyps. Methods -Fifty patients with CRC, 26 polyps and 53 with normal colonoscopy were included. Total and human DNA were analyzed from the frozen stools. Results -An increased concentration of hDNA in the stools was observed in colorectal cancer patients compared to controls and polyps. Tumors localized in the left side of the colon had higher concentrations of hDNA. There were no difference between polyps and controls. A cut off of 0.87 ng/mL of human DNA was determined for colorectal cancer patients by the ROC curve, with a sensitivity of 66% and a specificity of 86.8%. For polyps the cut off was 0.41, the sensitivity was 41% and the specificity 77.4%. Conclusion -A higher concentration of hDNA had been found in colorectal cancer patients The quantification of hDNA from the stools can be a trial method for the diagnosis of colorectal cancer.

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“…Calistri et al (6) , and Lidgard et al (15) , also did not find any difference among the different stages suggesting that it may be a screen method for precocious cancer.…”

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Human Dna Quantification in the Stools of Patients With Colorectal Cancer

Teixeira

Lima

Souza

et al. 2015

Arq. Gastroenterol.

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“…De plus, il existe environ 60 % de fauxpositifs liés à des saignements hémorroïdaires ou à la détec-tion de peroxydases alimentaires dans les selles. Dans ce contexte a été développé le MT-sDNA qui présente tous les éléments d'un test de dépistage efficace : sensibilité élevée pour les CCR et les lésions précancéreuses à haut risque, non affecté par le site tumoral (côlon droit ou gauche), indépen-dant de l'opérateur, non invasif, pas de préparation colique nécessaire [93]. Le test cible les mutations de KRAS, les anomalies de mutations de BMP3 et NDRG4, recherche la présence de β-actine (un marqueur de l'ADN humain total) ainsi que la présence de sang occulte par méthode immunochimique.…”

Section: Prévention -Marc-antoine Benderraunclassified

Congrès de l’association américaine de recherche contre le cancer — AACR 2015

Soares¹,

Michels

Hadoux

et al. 2015

Oncologie

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“…MT-sDNA has potential to meaningfully address current gaps in our approach to CRC screening through benefits of its high accuracy on screening effectiveness, user-friendly features on patient compliance, and easy mail-out distribution on test access. Furthermore, its highly reproducible automated testing avoids operator variation that compromises quality with endoscopic and subjective in vitro screening methods [10].…”

Section: Introductionmentioning

confidence: 99%

Multi-Target Stool DNA Test: A New High Bar for Noninvasive Screening

Ahlquist

2014

Dig Dis Sci

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Stool DNA testing has evolved into what is now a highly accurate and well-validated test for the screen detection of colorectal neoplasia. An optimized and automated multi-target stool DNA test (MT-sDNA) has achieved the same high point-sensitivities as reported for colonoscopy and significantly higher sensitivity than by fecal immunochemical blood testing for detection of early-stage cancer and advanced precancer. Thus, MT-sDNA sets a new high criterion standard for the noninvasive screen detection of colorectal neoplasia. With clinical application, MT-sDNA has potential to meaningfully address current gaps in our approach to CRC screening through benefits of its high accuracy on screening effectiveness, user-friendly features on patient compliance, and easy mail-out distribution on test access. The US Food and Drug Administration and Center for Medicaid and Medicare Services have recently completed their parallel reviews of MT-sDNA, which has just become available for clinical use. This review summarizes the recent clinical validation data on MT-sDNA, addresses central clinical questions at this front end of implementation, and touches on innovative future applications of stool DNA testing.

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Clinical Performance of an Automated Stool DNA Assay for Detection of Colorectal Neoplasia

Cited by 119 publications

References 15 publications

Human Dna Quantification in the Stools of Patients With Colorectal Cancer

Human Dna Quantification in the Stools of Patients With Colorectal Cancer

Congrès de l’association américaine de recherche contre le cancer — AACR 2015

Multi-Target Stool DNA Test: A New High Bar for Noninvasive Screening

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