Clinical performance of four multiplex real-time PCR kits detecting urogenital and sexually transmitted pathogens

Abstract

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Performance verification and clinical evaluation of the NAP-Fluo Cycler system for detecting five genital tract pathogens based on microfluidic technology

“…Additionally, the median bacterial load of MG was 42 times lower than that of CT, which may have contributed to the lower sensitivity of current MG detection methods, increasing the likelihood of false-negative results [ 19 ]. Several other MPCR assay kits reportedly also miss the detection of MG-positive samples [ 20 ]. Upon comparing LODs, we discovered that the system's LOD for MG was higher than that indicated in the reference kit's instructions (500 CCU/mL vs. 0.1 CCU/mL), which is likely the primary reason for the missed detection of weakly positive samples.…”

Simultaneous real-time PCR detection of nine prevalent sexually transmitted infections using a predesigned double-quenched TaqMan probe panel

“…vaginalis Real Time PCR Detection Kit (CerTest Biotec S.L., Zaragoza, Spain) [ 22 ]; and (ii) kits for detection of 7–9 STIs, such as Allplex STI Essential Assay Q (MH, UU) (Seegene, Seoul, Republic of Korea) including CT/MG/MH/NG/TV, Ureaplasma parvum (UP), U . urealyticum (UU) [ 23 ] or FTD STD9 kit (Fast Track Diagnostics, Junglinster, Luxembourg) including CT/NG/MG/TV/UU/UP/GV/HSV-1/HSV-2 [ 24 ]. Although a number of commercial multiplex real-time PCR kits have been developed so far, they are expensive (about 12–15 USD/test for nine STIs, as import cost into Vietnam) and do not cover the major nine STIs found in Vietnam, which makes them unsuitable for screening purposes in hospitals and centers for disease control (CDCs) in developing countries.…”

“…Molecular tests, such as real-time polymerase chain reaction (PCR) and hybridizationbased mini-array, are more advantageous because they offer shorter turnaround time than the conventional methods [8][9][10][11][12][13][14][15]. Representative commercially available kits for multiplex realtime PCR detection of common STIs include (i) kits for the detection of 2-3 STIs such as Abbott m2000 RealTime CT/NG (Abbott Molecular Inc., Des Plaines, IL, USA) [16], Cobas Amplicor CT/NG test (Roche Molecular Diagnostics, Branchburg, NJ, USA) [17,18], Aptima Combo 2 Assay for CT/NG (Hologic/Gen-Probe, San Diego, CA, USA) [19], BD ProbeTec CT/GC Q x Amplified DNA assay (Becton Dickinson, Sparks, MD, USA) [20], Xpert CT/NG Assay (Cepheid, Sunnyvale, CA, USA) [21], and Certest VIASURE C. albicans, G. vaginalis, and T. vaginalis Real Time PCR Detection Kit (CerTest Biotec S.L., Zaragoza, Spain) [22]; and (ii) kits for detection of 7-9 STIs, such as Allplex STI Essential Assay Q (MH, UU) (Seegene, Seoul, Republic of Korea) including CT/MG/MH/NG/TV, Ureaplasma parvum (UP), U. urealyticum (UU) [23] or FTD STD9 kit (Fast Track Diagnostics, Junglinster, Luxembourg) including CT/NG/MG/TV/UU/UP/GV/HSV-1/HSV-2 [24]. Although a number of commercial multiplex real-time PCR kits have been developed so far, they are expensive (about 12-15 USD/test for nine STIs, as import cost into Vietnam) and do not cover the major nine STIs found in Vietnam, which makes them unsuitable for screening purposes in hospitals and centers for disease control (CDCs) in developing countries.…”

Prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium among Patients with Urogenital Symptoms in Istanbul

“…Increased rates of co-infections have been reported together with the use of PCR-based tests because otherwise the detection would be expensive and time-consuming. NAATs are helpful not only for the diagnosis but for clarification of the epidemiology and burden of sexually transmitted diseases (STDs) and also for advancing the knowledge of infections which are caused mainly by pathogens such as mycoplasma species that are difficult to investigate using conventional methods [ 15 , 16 , 17 ]. The present study aimed to investigate the presence of CT, NG, and MG in the urogenital specimens collected from 18–68-year-old patients with urogenital symptoms using a multiplex real-time PCR test.…”