Clinical Potential of a Silk Sericin-Releasing Bioactive Wound Dressing for the Treatment of Split-Thickness Skin Graft Donor Sites

Siritientong, Tippawan; Angspatt, Apichai; Ratanavaraporn, Juthamas; Aramwit, Pornanong

doi:10.1007/s11095-013-1136-y

Cited by 96 publications

(62 citation statements)

References 30 publications

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“…Sericin has been previously reported to promote attachment and proliferation of various mammalian cells by the activation of collagen production. [20][21][22][23][24][25] Furthermore, we previously demonstrated that sericin influenced on collagen production of cells in a concentration-dependent manner. 26 The higher concentration of sericin activated cells to produce more collagen.…”

Section: Resultsmentioning

confidence: 95%

“…20 We have intensively worked with various kinds of sericin-containing scaffolds aimed to be used as wound dressing materials. [21][22][23][24][25] The accelerated healing of wounds as a result of sericin released from the scaffolds was reported. Surprisingly, we preliminary found that sericin blended with CMC, without the use of any crosslinking agent, could be fabricated into a stable hydrogel even they are both water soluble polymers.…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of carboxymethyl cellulose/sericin hydrogels and the influence of molecular weight of carboxymethyl cellulose

Siritientong

Aramwit

2015

Macromol. Res.

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Carboxymethyl cellulose (CMC),which is a hydrophilic polysaccharide obtained from the carboxymethylation of natural cellulose, has been formulated as hydrogel for various biomedical applications. However, the functional crosslinking agents were required to form the stable CMC hydrogels. In this study, sericin which is a glue-like protein that envelops the fibroin fiber in the silkworm cocoons was introduced to blend with CMC to form a mechanically stable hydrogel without further crosslinking. Furthermore, the effect of molecular weight of CMC on characteristics of the CMC/sericin hydrogels obtained was investigated. We found that the incorporation of sericin in CMC hydrogels enhanced the stability and the hydrogel's resistance to hydrolysis and enzymatic degradation while the porous structure and swelling abilities of the CMC hydrogels were preserved. The in vitro biodegradation of the CMC/sericin hydrogels and the release of sericin from the hydrogels were evaluated. The biodegradability of the CMC/sericin hydrogels depended on the molecular weight of CMC. The high molecular weight CMC/sericin hydrogels showed the slowest rate of degradation. The slow-degrading high molecular weight CMC/sericin hydrogels resulted in the lowest amount of sericin release while the fast-degrading low and medium molecular weight CMC/sericin hydrogels released high amount of sericin. The high amount of sericin released from the fast-degrading low and medium molecular weight CMC/sericin hydrogels could consequently promote the collagen production of L929 cells. These CMC/sericin hydrogels could be further investigating to be applied as a wound dressing material.

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Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Characteristics of carboxymethyl cellulose/sericin hydrogels and the influence of molecular weight of carboxymethyl cellulose

Siritientong

Aramwit

2015

Macromol. Res.

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“…Sericin is more hydrophilic than fibroin and can more effectively induce collagen synthesis in dermal tissue. It is a good candidate for use in tissue engineering scaffolding as a dressing material or skin substitute [49]. Sericin film increases the proliferation of human skin keratinocytes and fibroblasts, and it accelerates wound healing [49].…”

Section: Sericinmentioning

confidence: 99%

“…It is a good candidate for use in tissue engineering scaffolding as a dressing material or skin substitute [49]. Sericin film increases the proliferation of human skin keratinocytes and fibroblasts, and it accelerates wound healing [49]. Sericin also has antimicrobial, antioxidant, and photo-protective ability from ultraviolet radiation, so it has been effectively used as a dressing material for skin protection [50,51].…”

Section: Sericinmentioning

confidence: 99%

Silk Protein-Based Membrane for Guided Bone Regeneration

Kwon

Seok

2018

Applied Sciences

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Silk derived from the silkworm is known for its excellent biological and mechanical properties. It has been used in various fields as a biomaterial, especially in bone tissue engineering scaffolding. Recently, silk protein-based biomaterial has been used as a barrier membrane scaffolding for guided bone regeneration (GBR). GBR promotes bone regeneration in bone defect areas using special barrier membranes. GBR membranes should have biocompatibility, biodegradability, cell occlusion, the mechanical properties of space-making, and easy clinical handling. Silk-based biomaterial has excellent biologic and mechanical properties that make it a good candidate to be used as GBR membranes. Recently, various forms of silk protein-based membranes have been introduced, demonstrating excellent bone regeneration ability, including osteogenic cell proliferation and osteogenic gene expression, and promoting new bone regeneration in vivo. In this article, we introduced the characteristics of silk protein as bone tissue engineering scaffolding and the recent application of such silk material as a GBR membrane. We also suggested future studies exploring additional uses of silk-based materials as GBR membranes.

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“…Sericin is also found to promote the activities of keratinocytes and primary cultured human fibroblasts as well as to enhance epithelialization and collagen formation (10)(11)(12)(13)(14). We have previously reported the accelerated wound healing rate in both animal and clinical trials by the application of various kinds of wound dressing releasing sericin, e.g., sericin/PVA scaffold and sericin-incorporated silk fibroin/gelatin scaffold (15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Controlled Release of Chitosan and Sericin from the Microspheres-Embedded Wound Dressing for the Prolonged Anti-microbial and Wound Healing Efficacy

Aramwit

Yamdech

Ampawong

2016

AAPS J

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One approach in wound dressing development is to incorporate active molecules or drugs in the dressing. In order to reduce the frequency of dressing changes as well as to prolong wound healing efficacy, wound dressings that can sustain the release of the active molecules should be developed. In our previous work, we developed chitosan/sericin (CH/SS) microspheres that released sericin in a controlled rate. However, the difficulty of applying the microspheres that easily diffuse and quickly degrade onto the wound was its limitations. In this study, we aimed to develop wound dressing materials which are easier to apply and to provide extended release of sericin. Different amounts of CH/SS microspheres were embedded into various compositions of polyvinyl alcohol/gelatin (PVA/G) scaffolds and fabricated using freeze-drying and glutaraldehyde crosslinking techniques. The obtained CH/SS microspheres-embedded scaffolds with appropriate design and formulation were introduced as a wound dressing material. Sericin was released from the microspheres and the scaffolds in a sustained manner. Furthermore, an optimized formation of the microspheres-embedded scaffolds (2PVA2G+2CHSS) was shown to possess an effective antimicrobial activity against both gram-positive and gram-negative bacteria. These microspheres-embedded scaffolds were not toxic to L929 mouse fibroblast cells, and they did not irritate the tissue when applied to the wound. Finally, probably by the sustained release of sericin, these microspheres-embedded scaffolds could promote wound healing as well as or slightly better than a clinically used wound dressing (Allevyn®) in a mouse model. The antimicrobial CH/SS microspheres-embedded PVA/G scaffolds with sustained release of sericin would appear to be a promising candidate for wound dressing application.

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Clinical Potential of a Silk Sericin-Releasing Bioactive Wound Dressing for the Treatment of Split-Thickness Skin Graft Donor Sites

Abstract: We introduce this novel silk sericin-releasing wound dressing as an alternative treatment for split-thickness skin graft donor sites.

Cited by 96 publications

References 30 publications

Characteristics of carboxymethyl cellulose/sericin hydrogels and the influence of molecular weight of carboxymethyl cellulose

Characteristics of carboxymethyl cellulose/sericin hydrogels and the influence of molecular weight of carboxymethyl cellulose

Silk Protein-Based Membrane for Guided Bone Regeneration

Controlled Release of Chitosan and Sericin from the Microspheres-Embedded Wound Dressing for the Prolonged Anti-microbial and Wound Healing Efficacy

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