Kidney stones are raising due to rapid industrialization. Stone formation is usually a result of urinary super saturation with the group of biominerals. We have evaluated clinical profile of patient with urolithiasis and analyse chemical composition of stone. We had studied 70 urolithiasis patients at department of general surgery, Bharati Vidyapeeth University Medical College, in Pune city.A male to female's ratio was 1.33:1. Most of patients had pain in left lumbar region (n=33, 47.14%). Radiating back pain to back and groin was observed in 49 patients. Haematuria was the commonest problem in patients (n=38, 54.29%). The most of patients were drinking tap water (n=53, 75.71%). Maximum number of patient were reported for radio opaque shadow in left kidney (n= 21, 30%) and then right kidney (n= 15, 21.43%). We reported high level of serum creatinine, calcium, and phosphorus level than the normal range of parameters. Acinetobacter, Enterococccus species, EscherichiaColi, Gram negative bacilli, Klebsiellapneumonia, Pseudomonas species and Trichosporon species were found in few patients. Calcium oxalate monohydrate content was found 63.99±16.05%. Calcium dihydrate (44.55±6.105%), ammonium urate (28.24±10.74%) and calcium phosphate (47.81±16.63%) were also present in the stone. Dicalciumphosphtate was present in 9 stones and carbon apatite was present in 10 stones. Our study shows the current status of chemical composition of stones in urolithiasis patients. It is important to know the chemical composition of stone as it is useful in advising people for taking preventive measures for reducing the risk of prevalence and recurrence of stone.