Clinical Protocols for the Evaluation of Rod Function

Abstract

This work presents a quick clinical protocol for dark-adapted chromatic (DAC) perimetry as well as a novel clinical tool, scotopic chromatic pupil campimetry (CPC). The goal of the study was to explore the applicability of these methods in a clinical setting, their test-retest repeatability, and the congruence of the results. Local rod sensitivity was assessed at 36 locations within 30° eccentricity of the visual field in 15 healthy subjects (mean age 43 ± 16 years; 7 females and 8 males) with DAC perimetry (red and cyan stimuli) and CPC 2 times in repeated measurements. The duration of individual measurements was 370 ± 5 s for CPC and 366 ± 62 s for DAC perimetry. The intraclass correlation (ICC) coefficient was 0.53 for DAC perimetry cyan stimuli, 0.67 for red stimuli, and 0.93 for CPC. However, the spatial resolution of CPC was substantially smaller than in DAC perimetry. We did not find a correlation of DAC perimetry and CPC measurements on the global or the local level. In comparison to DAC perimetry, CPC shows a superior intervisit repeatability in detecting functional changes in the rod population in an objective way with lower spatial resolution. Our results also indicate that these 2 methods measure the rod function in different ways and could thus constitute complementary scotopic functional diagnostics.

Clinical Phenotype of PDE6B-Associated Retinitis Pigmentosa

“…The responses in dark-adapted ff-ERG were detected in one patient in our cohort, obtained with 3 and 10-cds/m 2 light flashes. Yet, we judged these responses to be driven by cones [ 38 ]. The VF area obtained with target III4e in this patient was considerably large in both eyes; yet, there were two patients with even larger III4e VF areas with absent dark-adapted ff-ERG responses.…”

Spatial and temporal resolution of the photoreceptors rescue dynamics after treatment with voretigene neparvovec

“…Besides classical functional tests such as FST and visual acuity, we applied dark-adapted chromatic perimeter (DAC) with a new protocol and chromatic pupil campimetry (CPC). 18 These novel protocols can serve as clinical tools specifically tailored for evaluation of rod and cone responses after treatment of outer retinal diseases.…”

“…The scotopic CPC and the shortened protocol for DAC show a high reliability of both methods with a complementary ability to evaluate different aspects of the rod rescue. [18][19][20][21][22] on February 1, 2021 by guest. Protected by copyright.…”

Räumliche und zeitliche Auflösung der Wiederherstellungsdynamik der Photorezeptoren nach Behandlung mit Voretigen Neparvovec

“…Darüber hinaus wollten wir herausfinden, ob es Faktoren gibt, mit deren Hilfe sich der Behandlungseffekt vorhersagen lässt. Neben den klassischen Funktionsuntersuchungen wie FST und Visusbestimmung kamen dabei die dunkeladaptierte chromatische Perimetrie (DAC) mit einem neuen Protokoll und die chromatische Pupillenkampimetrie (CPC) zur Anwendung [18]. Diese neuartigen Protokolle können als klinische Instrumente dienen, die speziell auf die Beurteilung der Reaktion von Stäbchen und Zapfen nach der Behandlung von Erkrankungen der äußeren Netzhaut zugeschnitten sind.…”

“…Beide Methoden, die skotopische CPC und das gekürzte DAC-Protokoll, weisen eine hohe Zuverlässigkeit auf und ergänzen einander in der Fähigkeit zur Beurteilung verschiedener Aspekte der Stäbchenwiederherstellung [18-22].…”