Clinical relevance of IgE to recombinant Gly m 4 in the diagnosis of adult soybean allergy

Fukutomi, Yuma; Sjölander, Sigrid; Nakazawa, Takahiro; Borres, Magnus P.; Ishii, Toyota; Nakayama, Satoshi; Taniguchi, Masami; Saito, Akemi; Yasueda, Hiroshi; Nakamura, Hiroyuki; Akiyama, Kazuo

doi:10.1016/j.jaci.2012.01.031

Cited by 53 publications

(49 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, because of crossreactivity between crude allergen extracts from different fungi [9], apparent sensitization to crude Af extract does not always indicate genuine Af-sensitization. Recently, allergen components purified from their native sources or produced as recombinant proteins have been introduced into the battery of tests available for the diagnosis of allergic diseases [10][11][12][13], generally known as component-resolved diagnostics (CRD) [14][15][16], more recently, molecular-based allergy (MA) diagnostics [17]. Such techniques and diagnostics may solve this problem; however, clinical studies in MA diagnostics for fungal allergy have thus far been limited [18].…”

Section: Introductionmentioning

confidence: 99%

Molecular‐based allergy diagnosis of allergic bronchopulmonary aspergillosis in Aspergillus fumigatus‐sensitized Japanese patients

Tanimoto

Fukutomi

Yasueda

et al. 2015

Clin Experimental Allergy

View full text Add to dashboard Cite

SummaryBackgroundDistinguishing between patients with allergic bronchopulmonary aspergillosis (ABPA) and Aspergillus fumigatus (Af)‐sensitized asthmatic patients without ABPA is sometimes difficult owing to the IgE‐cross‐reactivity between Af and other fungal allergens.ObjectiveTo establish the usefulness of molecular‐based allergy diagnostics using allergen components from Af in distinguishing ABPA from Af‐sensitized asthma without ABPA.MethodsSera from Japanese patients with ABPA (n = 53) and Af‐sensitized asthma without ABPA (n = 253) were studied. The levels of IgE and IgG antibodies to allergen components from Af and IgE antibodies to different fugal allergen extracts were measured by ImmunoCAP. Comorbid atopic dermatitis (AD) was taken into consideration in the sensitization profile analysis.ResultsPatients with ABPA possessed significantly higher levels of IgE antibodies to Asp f 1, and Asp f 2 than asthmatic patients without ABPA. The areas under the receiver operating characteristic curves for the levels of IgE to Asp f 1 and Asp f 2 as diagnostic markers of ABPA were 0.75 and 0.78, respectively. The presence of IgE positivity to Asp f 1 and/or Asp f 2 resulted in increased sensitivity while losing little specificity. Comorbid AD was associated with higher levels of IgE to Asp f 6 (manganese superoxide dismutase from Af, a ubiquitous pan‐allergen in fungi) and low but positive levels of IgE to other Af‐components, which hampered the serological discrimination of ABPA.Conclusions and Clinical RelevanceThe levels of IgE to Asp f 1 and/or Asp f 2 can effectively differentiate ABPA from Af‐sensitized asthma, suggesting that the amounts of IgE specific for these molecules are markers for genuine Af‐sensitization in ABPA. However, comorbid AD must be taken into consideration in the interpretation of high IgE to Asp f 6. Establishing of IgE‐sensitization profiles using panel of Af‐allergen components provides valuable information for distinguishing genuine vs. cross‐reactive sensitization in Af‐sensitized patients.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular‐based allergy diagnosis of allergic bronchopulmonary aspergillosis in Aspergillus fumigatus‐sensitized Japanese patients

Tanimoto

Fukutomi

Yasueda

et al. 2015

Clin Experimental Allergy

View full text Add to dashboard Cite

show abstract

“…IgE-binding to Gly m 1 has been reported in > 90 % of patients (Djurtoft et al, 1991), to Gly m 4 in 86 % (Baur et al, 1996), and to Gly m 3 in 69 % (Rihs et al, 1999). Later studies reported IgE to Gly m 4 in 70 % to 100 % of soy-allergic patients (Mittag et al, 2004b;Ballmer-Weber et al, 2007;Fukutomi et al, 2012). IgE to Gly m 5 and 6 was detected in 5 to 67 % and 5 to 58 % of patients, respectively Ito et al, 2011;Fukutomi et al, 2012).…”

Section: Identified Allergensmentioning

confidence: 98%

Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Products¹

2014

EFS2

117

View full text Add to dashboard Cite

Following a request from the Food Safety Authority of Ireland, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA Panel) was asked to deliver a scientific opinion on the evaluation of allergenic foods and food ingredients for labelling purposes. In view of the request, the NDA Panel decided to update its previous opinions relative to food ingredients or substances with known allergenic potential listed in Annex IIIa of 2003/89/EC, as amended. These include cereals containing gluten, milk and dairy products, eggs, nuts, peanuts, soy, fish, crustaceans, molluscs, celery, lupin, sesame, mustard and sulphites. The opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. It includes information on the prevalence of food allergy in unselected populations, proteins identified as food allergens, cross-reactivities, the effects of food processing on the allergenicity of foods and ingredients, methods for the detection of allergens and allergenic foods, doses observed to trigger adverse reactions in sensitive individuals and risk assessment methodologies that have been used to derive individual and population thresholds for selected allergenic foods. The present opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. For each food ingredient or substance listed in Annex IIIa, it includes information on the prevalence of food allergy in unselected populations, on proteins identified as food allergens, on cross-reactivities, on the effects of food processing on the allergenicity of foods and ingredients, on methods for the detection of allergens and allergenic foods, and on doses observed to trigger adverse reactions in sensitive individuals.Immune-mediated adverse reactions to foods manifest with clinical signs and symptoms of variable severity and duration, which may affect different organs and systems. Anaphylactic reactions to food are IgE mediated and may occur at any age. Non-IgE-mediated food allergy includes a wide range of diseases, including protein-induced enterocolitis and eosinophilic oesophagitis.A careful family and clinical history are the basis for diagnosis of food allergy. Food diaries, skin prick tests (SPTs), allergen-specific IgE measurements, food elimination diets and food challenges are part of the standard protocol for the diagnosis of food allergy. A positive SPT indicates sensitisation to the tested food, but it is not diagnostic of food allergy. Allergen-specific serum IgE antibodies similarly denote sensitisation to a particular food, but they are not diagnostic without a clinical history or food challenge. The use of atopy patch tests for the diagnosis of food allergy is controversial. Other available tests have no current role in the diagnosis of food allergy. Diag...

show abstract

“…5 In 2012, Fukutomi et al reported that, in their Japanese cohort, a high level of IgE to recombinant Gly m 4 was associated with adult soybean allergy. 6 More recently, Ebisawa et al reported that IgE to the 2S albumin Gly m 8 was significantly greater in children reactive to soy than those who were asymptomatic, while IgE levels to Gly m 5 and Gly m 6 were not. 7 Klemans et al also reported that Gly m 8 had the best accuracy in diagnosing soy allergy, although the AUC for this component was comparable to SPT and sIgE to soy extract.…”

Section: To the Editormentioning

confidence: 98%

Clinical reactivity to soy is best identified by component testing to Gly m 8

Kattan

Sampson

2015

The Journal of Allergy and Clinical Immunology: In Practice

View full text Add to dashboard Cite

Clinical relevance of IgE to recombinant Gly m 4 in the diagnosis of adult soybean allergy

Cited by 53 publications

References 8 publications

Molecular‐based allergy diagnosis of allergic bronchopulmonary aspergillosis in Aspergillus fumigatus‐sensitized Japanese patients

Molecular‐based allergy diagnosis of allergic bronchopulmonary aspergillosis in Aspergillus fumigatus‐sensitized Japanese patients

Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Clinical reactivity to soy is best identified by component testing to Gly m 8

Contact Info

Product

Resources

About