Clinical relevance of monitoring cytokine production following living donor renal transplantation

Basak, Udit; Mitra, Dipendra Kumar; Panigrahi, Ashutosh; Guleria, Sandeep; Agarwal, Sanjay Kumar; Mehta, Saurabh; Dash, Suvashis; Mehra, N. K.

doi:10.1016/s0041-1345(02)03897-6

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…On the other hand, quantitative evaluation of soluble interleukin receptor (sIL‐2R) has also been found to be useful in the detection of rejection in recipients of kidney and heart allografts (5,6). Recent studies have substantiated that immunologic monitoring of antidonor antibodies and cytokines in peripheral blood, serum and urine could be a predictive marker to improve diagnosis and treatment of allograft rejection (7–9). The objective of this study is to analyze the clinical relevance of immune parameters following live‐related renal transplantation by correlating allograft survival with donor‐specific anti‐HLA antibodies, intracellular expression of cytokines and sIL‐2R involved in immune response, which are the major predictors of graft outcome following renal transplantation.…”

mentioning

confidence: 99%

Functional assessment of immune markers of graft rejection: a comprehensive study in live‐related donor renal transplantation

Panigrahi¹,

Deka²,

Bhowmik³

et al. 2005

Clinical Transplantation

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A better understanding of the immunobiological processes and predictors of graft rejection holds promise for the development of potential therapeutic strategies and also individualization of immunosuppression. The objective of this study is to analyze the clinical relevance of immune parameters such as antidonor antihuman leukocyte antigen (anti-HLA) antibodies, monitoring of cytokines and their receptors on the graft outcome following live-related donor renal transplantation. Flow cytometry-based methods were used to detect antidonor antibodies (flow cytometry crossmatch, FCXM) and intracellular cytokines. Enzyme-linked immunosorbent assay (ELISA) methods were employed to detect anti-HLA class I and class II antibodies and quantitative serum-soluble interleukin-2 receptor (sIL-2R) levels. The data revealed that patients with HLA class I-specific IgG antibody experienced higher acute rejection (AR) episodes at 1 yr in comparison to the antibody negative group (82% vs. 56%, p = 0.01). On the contrary, donor-specific class II antibodies (B+) did not have any influence on the graft survival. However, 15 recipients having both T- and B-cell antidonor antibodies (T+B+) had significantly poor graft survival (60%) as compared to the antibody-negative group (T-B-, 82%, p = 0.05). Additionally, patients having non-donor but HLA-specific antibodies (FCXM-/ELISA+) had poor graft survival as compared to the antibody-negative group (64% vs. 88%, p < 0.05). Further, patients undergoing AR episodes had significantly higher expression of IFN-gamma-producing T cells (19.16 +/- 7.4% median 17.50) as compared to their pre-transplant levels (5.68 +/- 1.63%, Median 5.20) and the non-rejecter group (5.97 +/- 4.39%, median 4.3, p = 0.0004). Similarly sIL-2 was significantly increased in AR episodes during the first month of transplantation (292 +/- 131.5 pmol/L) as compared to those with well-functioning grafts (p = 0.01) and healthy controls (p = 0.001). Evaluation of antidonor antibodies by flow cytometry is found to be relatively more sensitive and a better predictor of graft outcome. Further monitoring of cytokine expression profile of primed peripheral T-helper cells and quantitative analysis of sIL-2R offer additional valuable diagnostic and prognostic tools for follow-up of transplant subjects and a better alternative for functional assessment of immunosuppression.

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confidence: 99%

Functional assessment of immune markers of graft rejection: a comprehensive study in live‐related donor renal transplantation

Panigrahi¹,

Deka²,

Bhowmik³

et al. 2005

Clinical Transplantation

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“…It has been established in human medicine and through the use of experimental models that cytokines play an important role in immune defense, immunopathologic processes, and allograft rejection. Although information exists for animals used in transplantation experiments 37,38,49,50 as well as for in vivo 15,17,23,28,29,51 and in vitro 25,52,53 studies in humans regarding the effects of immunosuppressive treatment on cytokines critical to the rejection process, limited information currently exists for these effects in cats. In 1 report, 54 the effects of cyclosporine on the expression of inflammatory cytokines for PBMCs in 5 cats was evaluated by the use of Con A as a stimulus.…”

Section: Discussionmentioning

confidence: 99%

“…[15][16][17][18][19][20][21][22] Several cytokines (eg, IFN-γ, IL-2, IL-4, IL-10, and GM-CSF) have been implicated in experimental animals and in humans as playing a role in acute and chronic allograft rejection. [23][24][25][26][27][28] The Th1 cytokines (including IFN-γ and IL-2) contribute to rejection by activating the cellular immune response (including cytotoxic T cells, monocytes, and natural killer cells). It is believed that IL-2 is a driving force for acute cellular rejection; however, it is only 1 component of the rejection process.…”

mentioning

confidence: 99%

Effect of cyclosporine, dexamethasone, and human CTLA4-Ig on production of cytokines in lymphocytes of clinically normal cats and cats undergoing renal transplantation

Aronson

Stumhofer²,

Drobatz³

et al. 2011

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“…Cytokines are important players during the functioning of immune cells, in both intracellular signal transduction and intercellular communications [ 6 – 8 ]. Luminex xMAP technology makes it possible to simultaneously detect up to a hundred of cytokines in a single 12 μl sample [ 9 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

Experimental immunology Cytokine signatures of human whole blood for monitoring immunosuppression

He¹,

Luo

Lao³

et al. 2014

cejoi

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How to evaluate status of the immune system is extremely critical for clinical immunosuppressive treatment. In this study, we tested the secretion of cytokines in undiluted whole blood samples stimulated with Phorbol 12-myristate 13-acetate (PMA) and ionomycin (IONO), and compared the effects of dexamethasone (DEX), cyclosporine A (CsA) or mycophenolic acid (MPA), either alone or in combination, on cytokine profiles. The results showed that both DEX and CsA dose-dependently inhibited the production of eleven cytokines: interleukin (IL)-2, IL-4, IL-5, IL-6, IL-13, IL-17, interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). Unexpectedly, MPA showed no obvious influences except for the mild inhibition on GM-CSF production. In combination treatment, cytokine profiles reflect not only the synergistic effects among drugs, but also the specific effect of the individual drug. Thus, the effects of different immunosuppressants could be reflected through their specific cytokine signatures, which can be applied to maximize immunosuppressive effects, while to minimize risk of infections and help physicians to reasonably apply immunosuppressants.

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Clinical relevance of monitoring cytokine production following living donor renal transplantation

Cited by 5 publications

References 15 publications

Functional assessment of immune markers of graft rejection: a comprehensive study in live‐related donor renal transplantation

Functional assessment of immune markers of graft rejection: a comprehensive study in live‐related donor renal transplantation

Effect of cyclosporine, dexamethasone, and human CTLA4-Ig on production of cytokines in lymphocytes of clinically normal cats and cats undergoing renal transplantation

Experimental immunology Cytokine signatures of human whole blood for monitoring immunosuppression

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