Clinical Relevance of the Recently Described Species Aeromonas aquariorum

Abstract

Twenty-two human extraintestinal isolates (11 from blood) and three isolates recovered from patients with diarrhea were genetically characterized as Aeromonas aquariorum, a novel species known only from ornamental fish. The isolates proved to bear a considerable number of virulence genes, and all were resistant to amoxicillin (amoxicilline), cephalothin (cefalotin), and cefoxitin. Biochemical differentiation from the most relevant clinical species is provided.

Aeromonas dhakensis pneumonia and sepsis in a neonate Risso’s dolphin Grampus griseus from the Mediterranean Sea

“…The expected genus-characteristic resistance against ampicillin was observed for our A. dhakensis strain. Also the results obtained for the susceptibility analysis of the rest of the antimicrobials tested agreed with those reported by other authors, showing that the cephalo sporins (third and fourth generation), monobactams, carbapenems, aminoglycosides, fluoroquinolones, sulphonamides, polypeptides and amphenicols tes ted were highly effective against this microorganism (Figueras et al 2009, Esteve et al 2012, Wu et al 2012, Chen et al 2013, Morinaga et al 2013.…”

“…This bacterium was also occasionally isolated from stranded elephant seals, California sea lions and harbour seals (Thornton et al 1998). It is possible that the A. hydrophila identified phenotypically in those studies could belong, after a genetic identification, to A. dhakensis (synonym of A. aquariorum), as occurred in previous studies (Figueras et al 2009, Aravena-Román et al 2011, Morinaga et al 2013). In our case, pathologic and bac teriologic examination revealed an acute haemorrhagic-necrotizing pneumonia and sepsis associated with A. dhakensis, which was recovered in pure culture from lung, liver, spleen, kidney and blood.…”

“…Studies in marine mammals that use these phenotypic identification methods to characterize Aero monas isolates will probably uncover the prevalence of A. dhakensis under A. hydrophila. However, A. dhakensis can be easily recognised using molecular identification with the sequences of housekeeping genes as shown in different studies (Figueras et al 2009, Beaz-Hidalgo et al 2010, AravenaRomán et al 2011, Esteve et al 2012, Puthucheary et al 2012, Sedlá ek et al 2012, Wu et al 2012, Chen et al 2013, Morinaga et al 2013, Soto-Rodriguez et al 2013. The housekeeping genes rpoD and gyrB were shown to be very useful .…”

Characterization and antimicrobial susceptibility of motile aeromonads isolated from freshwater ornamental fish showing signs of septicaemia

“…The 2 A. dhakensis isolates shared an atypical pattern that is closely related to A. caviae. Figueras et al (2009) reported that A. dhakensis produce either the A. caviae RFLP pattern or a somewhat similar pattern with extra bands.…”

Distribution and phenotypic and genotypic detection of a metallo-β-lactamase, CphA, among bacteraemic Aeromonas isolates

“…Overall, 51 blood isolates were identified as A. aquariorum (16, 31 %), A. caviae (15, 29 %), A. veronii (12, 24 %), A. hydrophila (5, 10 %) and Aeromonas species (3, 4 %). Some of the isolates had been described previously (Wu et al, 2007;Figueras et al, 2009). The GenBank accession numbers for the rpoB gene sequences of A. aquariorum MDC47 T and 11 published A. aquariorum blood isolates are JF972604-JF972615.…”