Clinical results of the application of a hemoglobin spray to promote healing of chronic wounds

Arenberger, P.; Engels, Peter; Arenbergerová, Monika; Gkalpakiotis, Spyridon; Martínez, Francisco Javier García Luna; Anaya, Americo Villarreal; Fernandez, Laura Jimenez

doi:10.3205/dgkh000162

Cited by 7 publications

(2 citation statements)

References 9 publications

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“…Bovine hemoglobin was demonstrated as a pH-sensitive nano-vehicle for potential cancer detection and therapy [8] and as an effective glucose biosensor in vitro [9]. Via facilitating oxygen diffusion, porcine hemoglobin isolated from slaughterhouse blood also appeared as an effective wound healing spray agent [10,11]. According to data from the literature, products based on hemoglobin, aimed to be used as a component of cell culture media, have been developed using only invertebrates' hemoglobin so far.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin

et al. 2021

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Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs >in vivo.

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Section: Introductionmentioning

confidence: 99%

Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin

et al. 2021

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“…Apart from the use as blood substitute, porcine hemoglobin was used as a wound healing agent in the form of spray if applied at regular intervals of 2–4 weeks. Namely, this product has been shown to reduce pain, wound size, and the necrotic tissue layer that impairs wound healing [ 67 , 68 ]. Moreover, there are reports on bovine hemoglobin used as a drug platform itself, i.e., as a pH-sensitive nano-vehicles for potential cancer detection and therapy [ 69 ] and as an effective glucose biosensor in vitro [ 70 ].…”

Section: Exogenous Administration Of Extracellular Hemoglobinmentioning

confidence: 99%

Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects

et al. 2022

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Hemoglobin is essential for maintaining cellular bioenergetic homeostasis through its ability to bind and transport oxygen to the tissues. Besides its ability to transport oxygen, hemoglobin within erythrocytes plays an important role in cellular signaling and modulation of the inflammatory response either directly by binding gas molecules (NO, CO, and CO2) or indirectly by acting as their source. Once hemoglobin reaches the extracellular environment, it acquires several secondary functions affecting surrounding cells and tissues. By modulating the cell functions, this macromolecule becomes involved in the etiology and pathophysiology of various diseases. The up-to-date results disclose the impact of extracellular hemoglobin on (i) redox status, (ii) inflammatory state of cells, (iii) proliferation and chemotaxis, (iv) mitochondrial dynamic, (v) chemoresistance and (vi) differentiation. This review pays special attention to applied biomedical research and the use of non-vertebrate and vertebrate extracellular hemoglobin as a promising candidate for hemoglobin-based oxygen carriers, as well as cell culture medium additive. Although recent experimental settings have some limitations, they provide additional insight into the modulatory activity of extracellular hemoglobin in various cellular microenvironments, such as stem or tumor cells niches.

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Aging, Hypercoagulability, and Leg Necrosis in Critical Limb Ischemia

Park

Bae

Kim

et al. 2015

Annals of Vascular Surgery

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Clinical results of the application of a hemoglobin spray to promote healing of chronic wounds

Cited by 7 publications

References 9 publications

Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin

Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin

Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects

Aging, Hypercoagulability, and Leg Necrosis in Critical Limb Ischemia

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