Clinical Role of Soluble Adhesion Molecules During Immunotherapy for Perennial Allergic Rhinitis

Ōhashi, Y.; Nakai, Yousuke; Tanaka, Ayaki; Kakinoki, Yasushi; Ohno, Yoshiharu; Masamoto, Tateo; Sakamoto, Hirokazu; Kato, Akifumi; Washio, Yushi; Yamada, Kôji; Hayashi, Motoyo

doi:10.1001/archotol.124.1.41

Cited by 4 publications

(5 citation statements)

References 23 publications

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“…Secrist et al [15] demonstrated that production of IFN-g by allergen-specific CD4 þ T cells was not affected in grass pollen and mite allergic subjects on immunotherapy whereas IL-4 production was reduced by immunotherapy and that the levels of IL-4 produced are inversely related to the length of time on immunotherapy. We demonstrated previously that serum levels of IL-4 in patients with perennial allergic rhinitis were decreased by immunotherapy [23,39]. In our previous serological study for patients with seasonal allergic rhinitis, pollen immunotherapy reduced a seasonal activation of Th2 response and additionally switched the seasonal preferential activation of Th2 cells to reciprocal activation of Th1 cells with treatment over several years [14].…”

Section: Discussionmentioning

confidence: 84%

Immunotherapy Suppresses both Th1 and Th2 Responses by Allergen Stimulation, but Suppression of the Th2 Response is a More Important Mechanism Related to the Clinical Efficacy of Immunotherapy for Perennial Allergic Rhinitis

et al. 1998

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Increased attention has recently been directed at the possibility that the clinical efficacy of immunotherapy might be elaborated by alteration of T-cell reactivity. However, there is no general agreement among different investigators regarding the effect of immunotherapy on Th-cell reactivity. Peripheral blood mononuclear cells (PBMCs) from 15 nonatopic subjects and 76 patients with perennial allergic rhinitis (18 untreated patients and 58 patients on immunotherapy) were cultured in the absence and in the presence of a major Dermatophagoides farinae allergen, Der f 1, and the levels of IgE, interleukin-5 (IL-5), interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) in the culture supernatants were determined. The difference between the absence and presence of Der f 1 was calculated to consider the Der f 1-dependent synthesis of IgE, IL-5, IFN-gamma and TNF-alpha. The levels of Der f 1-dependent synthesis of IgE, IL-5 and TNF-alpha were significantly higher in the untreated group than in the nonatopic group, whereas Der f 1-dependent synthesis of IFN-gamma was significantly lower in the untreated group than in the nonatopic group. Immunotherapy decreased the enhanced Der f 1-dependent synthesis of IgE, IL-5 and TNF-alpha, and further decreased the suppressed Der f 1-dependent synthesis of IFN-gamma as the therapy proceeded. The levels of Der f 1-dependent synthesis of IgE and IL-5 did not differ between nonatopic individuals and patients whose duration of immunotherapy was 10 or more years. The levels of Der f 1-dependent synthesis of IgE and IL-5, but not of IFN-gamma and TNF-alpha, were correlated significantly with the levels of symptom scores. In addition, the levels of Der f 1-dependent synthesis of IgE and IL-5, but not of IFN-gamma and TNF-alpha, differed significantly between good and poor responders. In conclusion, immunotherapy for perennial allergic rhinitis may possibly work via induction of tolerance or anergy of both Th1- and Th2 cells. However, our study is likely to support a view that the mechanisms responsible for the clinically beneficial effects of immunotherapy principally involve the tolerance of Th2- rather than Th1 cells. In addition, suppression of IgE synthesis is also likely to be linked to the clinical efficacy of immunotherapy for perennial allergic rhinitis.

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Section: Discussionmentioning

confidence: 84%

Immunotherapy Suppresses both Th1 and Th2 Responses by Allergen Stimulation, but Suppression of the Th2 Response is a More Important Mechanism Related to the Clinical Efficacy of Immunotherapy for Perennial Allergic Rhinitis

et al. 1998

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“…Ohashi et al. (23) reported that the percentage decrease in sICAM‐1 concentration was significantly correlated with the duration of immunotherapy and with the percentage of the decrease in symptom scores in allergic rhinitis. Our data further demonstrated lower expression of CD54 and CD69 after immunotherapy in asthmatic children with a good response.…”

Section: Discussionmentioning

confidence: 99%

Reduced expression of CD69 and adhesion molecules of T lymphocytes in asthmatic children receiving immunotherapy

Huang

Tsao

et al. 2002

Pediatric Allergy Immunology

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T lymphocytes play a fundamental role in the initiation and regulation of chronic inflammatory responses in patients with asthma. CD69 is an early marker of T-cell activation. The levels of intercellular adhesion molecule-1 (ICAM-1, CD54) and L-selectin have been reported to increase in patients with allergic diseases and asthma. The present study was therefore undertaken to investigate the expression of CD69, CD54, and L-selectin by T lymphocytes of children with asthma, before and after immunotherapy. Eighteen children newly diagnosed with asthma, 11 good and nine poor responders to immunotherapy, and 16 normal subjects, were enrolled in this study. The percentages of CD69+, CD54+, and CD62L+ cells in T lymphocytes were measured by using flow cytometry. The levels of CD69, CD54, and CD62L in serum and culture supernatants were determined by using enzyme-linked immunosorbent assay (ELISA). The expression of CD69 and CD54 on CD3+ T lymphocytes was significantly higher in children with asthma than in control patients. All the patient groups expressed (spontaneously and following stimulation with phorbol myristate acetate and ionomycin together with mite-extract proteins) greater amounts of CD69 and CD54 than did control subjects. With long-term immunotherapy, the percentages of CD69+ and CD54+ T lymphocytes were significantly lower in patients with a good response to immunotherapy. Our results also showed significantly lower serum L-selectin levels following immunotherapy. In conclusion, successful immunotherapy resulted in decreased expression and production of CD69 and CD54. These results may explain, in part, the clinical efficacy of immunotherapy.

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“…The details of the method used in this study have been described elsewhere. 2,[6][7][8] In brief, all the patients were asked to complete daily symptom diary cards for 14 days before we assessed their nasal symptom scores. The daily symptom cards included the number of sneezing attacks and nose blows, and the degree of nasal obstruction.…”

Section: Clinical Assessment Of Symptom Scoresmentioning

confidence: 99%

“…The details and the justification of each method used in the present study have been described elsewhere. [6][7][8]12,13 The minimum sensitivity of each assay was 40 U/mL of soluble IL-2R, 40 ng/mL of soluble ICAM-1 and soluble VCAM-1, and 0.75 pg/mL of IL-4.…”

Section: Scoring Of Nasal Obstructionmentioning

confidence: 99%

“…27 Soluble forms of ICAM-1 and VCAM-1 have been identified in peripheral blood, and serum levels of these adhesion molecules were also reported to be higher in patients with allergic conditions. 8,16,28 Higher levels of these adhesion molecules in the serum of atopic individuals may reflect the up-regulation of cell-surface ICAM-1 and VCAM-1 expression in allergic inflammation. In the present study, immunotherapy significantly decreased levels of soluble ICAM-1.…”

Section: Commentmentioning

confidence: 99%

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Serologic Study of the Working Mechanisms of Immunotherapy for Children With Perennial Allergic Rhinitis

Ōhashi

Nakai²,

Tanaka³

et al. 1998

Arch Otolaryngol Head Neck Surg

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Clinical Role of Soluble Adhesion Molecules During Immunotherapy for Perennial Allergic Rhinitis

Abstract: Decrease in serum sICAM-1 levels during immunotherapy is probably involved in the working mechanisms of immunotherapy, but modulation of serum sVCAM-1 levels is not likely related to the clinical effect of immunotherapy.

Cited by 4 publications

References 23 publications

Immunotherapy Suppresses both Th1 and Th2 Responses by Allergen Stimulation, but Suppression of the Th2 Response is a More Important Mechanism Related to the Clinical Efficacy of Immunotherapy for Perennial Allergic Rhinitis

Immunotherapy Suppresses both Th1 and Th2 Responses by Allergen Stimulation, but Suppression of the Th2 Response is a More Important Mechanism Related to the Clinical Efficacy of Immunotherapy for Perennial Allergic Rhinitis

Reduced expression of CD69 and adhesion molecules of T lymphocytes in asthmatic children receiving immunotherapy

Serologic Study of the Working Mechanisms of Immunotherapy for Children With Perennial Allergic Rhinitis

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