Clinical scale ex vivo expansion of cord blood–derived outgrowth endothelial progenitor cells is associated with high incidence of karyotype aberrations

Corselli, Mirko; Parodi, Alessia; Mogni, Massimo; Sessarego, Nadia; Kunkl, Annalisa; Dagna-Bricarelli, F; Ibatici, Adalberto; Pozzi, Sarah; Bacigalupo, Andrea; Frassoni, Francesco; Piaggio, Giovanna

doi:10.1016/j.exphem.2007.10.008

Cited by 46 publications

(44 citation statements)

References 30 publications

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“…Although cord blood-BOEC have the advantages of higher proliferation and vascularization capacity [42], recent evidence suggests that this can be compensated for by preconditioning of peripheral bloodderived BOEC with angiogenic growth factors [43]. In addition, the more proliferative nature of cord blood-BOEC may cause cytogenetic abnormalities, which were not observed in peripheral blood-BOEC [44]. The need for exogenously added BOEC is underscored by the observation that the contribution of endogenous EC precursors is insignificant during wound vascularization [45].…”

Section: Discussionmentioning

confidence: 99%

Integration of Blood Outgrowth Endothelial Cells in Dermal Fibroblast Sheets Promotes Full Thickness Wound Healing

et al. 2010

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Vascularization is the cornerstone of wound healing. We introduced human blood outgrowth endothelial cells (hBOEC) in a self-assembled human dermal fibroblast sheet (hDFS), intended as a tissue-engineered dermal substitute with inherent vascular potential. hBOEC were functionally and molecularly different from early endothelial progenitor cells and human umbilical vein endothelial cells (HUVEC). hBOEC alone, unlike HUVEC, efficiently revascularized and re-oxygenated the wound bed, both by active incorporation into new vessels and by trophic stimulation of host angiogenesis in a dose-dependent manner. Furthermore, hBOEC alone, but not HUVEC, accelerated epithelial coverage and matrix organization of the wound bed. In addition, integration of hBOEC in hDFS not only further improved vascularization, epithelial coverage and matrix organization but also prevented excessive wound contraction. In vitro analyses with hBOEC, fibroblasts and keratinocytes revealed that these effects were both due to growth factor crosstalk and to short cutting hypoxia. Among multiple growth factors secreted by hBOEC, placental growth factor mediated at least in part the beneficial effects on keratinocyte migration and proliferation. Overall, this combined tissue engineering approach paves the way for clinical development of a fully autologous vascularized dermal substitute for patients with large skin defects that do not heal properly.

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Section: Discussionmentioning

confidence: 99%

Integration of Blood Outgrowth Endothelial Cells in Dermal Fibroblast Sheets Promotes Full Thickness Wound Healing

et al. 2010

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“…ECFCs were considered as EPC-derived cells, 7,19 which originated from adherent cell population after 14-21 days of culture. After 21 days, the median frequency of ECFCs was 0.12 colonies/10 6 mononuclear cells (range, 0.03-0.57).…”

Section: Isolation and Characterization Of Ecfcsmentioning

confidence: 99%

“…These cells will be referred to as ECFCs, as previously described. 7,19 The colonies were mechanically picked from the original plate and seeded on another gelatin-coated well with EGM2-10% FBS for expansion.…”

Section: Isolation and Characterization Of Epcsmentioning

confidence: 99%

Endothelial progenitor cell–dependent angiogenesis requires localization of the full-length form of uPAR in caveolae

Margheri

Chillà

Laurenzana

et al. 2011

Blood

109

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“…BOEC can either be derived from cord or AB, with the former showing higher proliferation potential. 55 1. Cord blood: Many studies highlight the excellent outgrowth of BOEC from CB (CB-BOEC) and suggest them, due to their high expansion and vasculogenic potential, to be ideal candidates for (S)TE purposes.…”

mentioning

confidence: 99%

“…Therefore, the application of CB-BOEC may not be free of risk for clinical application. 55 Moreover, in the absence of cell banking, autologous CB-BOEC are not available and as an allogeneic cell source are therefore at risk for immune rejection 23 (Table 1). 2.…”

mentioning

confidence: 99%

Cell-Based Vascularization Strategies for Skin Tissue Engineering

Hendrickx

Vranckx

Luttun

2011

Tissue Engineering Part B: Reviews

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Clinical scale ex vivo expansion of cord blood–derived outgrowth endothelial progenitor cells is associated with high incidence of karyotype aberrations

Cited by 46 publications

References 30 publications

Integration of Blood Outgrowth Endothelial Cells in Dermal Fibroblast Sheets Promotes Full Thickness Wound Healing

Integration of Blood Outgrowth Endothelial Cells in Dermal Fibroblast Sheets Promotes Full Thickness Wound Healing

Endothelial progenitor cell–dependent angiogenesis requires localization of the full-length form of uPAR in caveolae

Cell-Based Vascularization Strategies for Skin Tissue Engineering

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