2002
DOI: 10.1038/sj.bmt.1703406
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Clinical scale isolation of T cell-depleted CD56+ donor lymphocytes in children

Abstract: Summary:We present a clinical scale method for immunomagnetic separation of CD56 ϩ ; isolation; immunomagnetic separation; DLI; mismatched transplantation Transplantation of highly purified CD34 ϩ stem cells from mismatched related and (partially) matched unrelated donors is now a well established method in children with high risk or relapsed leukemias who otherwise lack an HLA-identical family donor. This procedure minimizes graft-versus-host disease (GVHD) without an increased risk of graft failure. However,… Show more

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Cited by 52 publications
(44 citation statements)
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“…This clinical scale method for isolation of T-cell-depleted CD56 þ donor NK-lymphocytes has been described previously, but purified NK donor lymphocyte have only been used rarely as adoptive immunotherapy after HSCT. 11,12 Whereas NK-cell purity was 97% and T-cell depletion was by 3.5 logs, we observed a considerable loss of NK cells during cell engineering. Overnight storing of collected cells may have contributed to this.…”
Section: Discussionmentioning
confidence: 66%
“…This clinical scale method for isolation of T-cell-depleted CD56 þ donor NK-lymphocytes has been described previously, but purified NK donor lymphocyte have only been used rarely as adoptive immunotherapy after HSCT. 11,12 Whereas NK-cell purity was 97% and T-cell depletion was by 3.5 logs, we observed a considerable loss of NK cells during cell engineering. Overnight storing of collected cells may have contributed to this.…”
Section: Discussionmentioning
confidence: 66%
“…Alloreactive proliferation in mixed lymphocyte cultures and response to T-cell-specific mitogen stimulation was profoundly decreased confirming extensive T-cell depletion. 44 The data on NK-DLI, obtained from haploidentical donors having previously undergone stem cell donation, may differ from data obtained in healthy volunteer donors. This may explain why the extent of T-cell depletion and NK-cell recovery was somewhat lower in our pilot protocol compared to other published data.…”
Section: Nk-cell Harvesting and Purificationmentioning
confidence: 99%
“…Another approach, which currently is being explored in the clinic, is to enhance NK cell-mediated ADCC by treating patients with IL-2 along with RTX (27,38,72). Finally, several methodologies are being developed to treat cancer patients by infusion of compatible NK cells (73)(74)(75)(76), and it would seem reasonable that patients could be given these NK cells to increase ADCC of RTX-opsonized malignant B cells.…”
Section: Parallel Findings In the Clinicmentioning
confidence: 99%